Mouse igm elisa kit

A bronchoalveolar lavage (BAL) was performed three times with 0.3 ml phosphate-buffered saline and then pooled. After counting the cell number, BAL cells were centrifuged on a slide and stained with the HemaDiff Quick Staining kit (Bioanalytic GmbH, Umkirch, Germany) for cytological investigations. Cell-free BAL fluid was obtained by centrifugation and analyzed for protein with the Pierce™ bicinchoninic acid (BCA) Protein Assay kit (Thermo Fisher Scientific, Rockford, IL), for IgM with the mouse IgM ELISA kit (Bethyl Laboratories, Montgomery, TX) and for soluble receptor for advanced glycation end-products (sRAGE) with the mouse RAGE DuoSet kit (R&D Systems, Minneapolis, MN). Heparinized blood was taken after transection of the vena cava and subjected to automated blood cell count using the scil Vet ABC™ Hematology Analyzer (scil animal care company GmbH, Viernheim, Germany).

Serum levels of antibodies to double stranded (ds) DNA (total IgA, IgG, and IgM) (Mouse anti-dsDNA antibodies total Ig ELISA Kit, Alpha Diagnostic, San Antonio, USA), IgM (Mouse IgM ELISA kit, Bethyl Laboratories, Montgomery, USA), IgG (Mouse IgG ELISA quantitation set, Bethyl Laboratories), interleukin-6 (IL-6) (Mouse IL-6 ELISA Kit, Life Technologies), urea (colorimetric Urea Assay Kit, Abcam, Cambridge, United Kingdom), C-terminal type I collagen (CTX-I, ELISA RatLaps kit, Immunodiagostic Systems, Boldon, UK), procollagen type I N propeptide (PINP, Immunodiagostic Systems), and parathyroid hormone (PTH, Elabscience, Houston, USA) were analyzed according to the manufacturers’ instructions.