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17 protocols using sodium acetate anhydrous

1

Biosynthesis of Iron Oxide Nanoparticles

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Ethylene glycol (EG), anhydrous sodium acetate (NaAc), ammonia solution (NH3·H2O, 28 wt.%), iron nitrate (Fe(NO3)3·9H2O), cetyltrimethylammonium bromide (CTAB), tetraethylorthosilicate (TEOS) were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai); Potassium dichromate (K2Cr2O7) and Dlphenylcarbazide were purchased from Aldrich; 4,4-Azobis(4-cyanovaleric acid) (ABCPA, 97%) and N-Acryloxysuccinimide was purchased from Sigma; Tryptone and yeast extract were supplied with Suzhou Biogene Biotechnology Co., Ltd. B. subtilis ATCC-6633 was obtained from Fujian Institute of Microbiology, China. All reagents were used as received without further purification.
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2

Fabrication of Protein-Functionalized Nanoparticles

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Ovalbumin (OVA), transferrin (Trf), horseradish peroxidase (HRP), lysozyme (Lyz) and bovine serum albumin (BSA) were obtained from the Beijing Solarbio Science and Technology Company. Ammonium bicarbonate (ABC), urea (urea), dithiothreitol (DTT), iodoacetamide (IAA), and trifluoroacetic acid (TFA) were purchased from J&K Scientific Ltd. 2-Methyl-imidazolate, 3-aminophenylboronic acid, and 3-isocyanatopropyltriethoxysilane were purchased from Aladdin. Iron(iii) chloride hexahydrate (FeCl3·6H2O, 99%), anhydrous sodium acetate (NaAc), ethylene glycol, zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), tetrahydrofuran, poly(sodium-p-styrenesulfonate) (PSS, 20 wt%), acetonitrile and ethanol were all purchased from Sinopharm Chemical Reagent Co., Ltd. Deionized water used for the experiments was ultrapure. All the reagents were analytically pure.
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3

Electrochemical Sensing of Heavy Metals

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MWCNTs (diameter 30 nm to 50 nm, purity ≥98%wt) were purchased from Chengdu Institute of Organic Sciences, Chinese Academy of Sciences, CS (high viscosity, ≥400 MPa · s) was obtained from Sigma Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China), and Pb 2+ ionophore IV (1053.59 g/mol) was purchased from Sigma Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China). Other reagents, including ice acetic acid (CH3COOH), anhydrous ethanol (CH3CH2OH), anhydrous sodium acetate (CH3COONa), cadmium nitrate (Cd(NO3)2), copper nitrate (Cu(NO3)2), potassium chloride (KCl), etc., were all provided by Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China). All the chemicals used were of analytical grade, and all ultrapure water used was deionized with a resistance of 18.25 MΩ · cm (megohm · cm). Electrochemical measurements were conducted by a CHI830D electrochemical workstation (Shanghai Chenhua Instrument, Shanghai, China) with a three-electrode chip (the working electrode was modified by MWCNT/CS/Pb 2+ ionophore IV) in a microcavity.
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4

Photocatalytic Degradation of Organic Pollutants

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4-carboxybenzene MOF (UiO-66) and 1,3,5-benzenetricarboxylic acid (H3BTC, ≥99%) were provided by Beijing J&K Technology Co., Ltd. Ferrocene (C10H10Fe), Fe(NO3)3·9H2O, methyl orange (MO, C14H14N3NaO3S), aqueous hydrogen peroxide solution (H2O2, 30 wt%), acetic acid (HAc), aniline, phenol, benzoic acid (BA), acetone and anhydrous sodium acetate (NaAc) were provided by Sinopharm Chemical Reagent Co, Ltd. All chemicals were of analytical grade and used without further treatment. The deionized (DI) water was used throughout the experiments.
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5

Biochemical Assay Reagents Protocol

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Sulphuric acid, methanol, anhydrous sodium acetate, sodium nitrate, and potassium dihydrogen phosphate (Analytical Reagent) were all purchased from Sinopharm Chemical Reagents Co., Ltd, Shanghai, China. NADH (100%) is obtained from Sigma-Aldrich, Germany. Formaldehyde solution, phenol, potassium chloride, sodium chloride, sodium disulfite, sodium nitrite, ammonium sulfate, anhydrous sodium dihydrogen phosphate, D-anhydrous glucose, disodium hydrogen phosphate, potassium chloride, iodonitrotetrazolium chloride (INT, 98%), and N-allyl thiourea (Analytical Reagent) were produced by Shanghai Macklin Biochemical Co., Ltd, China.
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6

Synthesis and Characterization of Aptamer-Functionalized Gold Nanoparticles

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Reagents and chemicals Chloroauric acid (HAuCl4) and streptavidin were obtained from Sigma-Aldrich (USA). Trisodium citrate, 1,6-hexanediamine, anhydrous sodium acetate, FeCl3•6H2O, glycol, and 25% glutaraldehyde (OHC(CH2)3CHO) were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The S. typhimurium aptamer were prepared in our laboratory. 26 The sequence of S. typhimurium aptamer is 5′-SH-ATAGGAGTCACGACGAC-CAGAAAGTAATGCCCGGTAGTTATTCAAAGATGAGTAG-GAAAAGATATGTGCGTCTACCTCTTGACTAAT-3′ (apt1), and 5′-Bio-ATAGGAGTCACGACGACCAGAAAGTAATGCC-CGGTAGTTATTCAAAGATGAGTAGGAAAAGATATGTGC-GTCTACCTCTTGACTAAT-3′ (apt2), synthesized by the Shanghai Sangon Biological Science & Technology Company (Shanghai, China).
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7

Sensitive Aflatoxin B1 Detection

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AFB1 standard solution, (8 mg•mL -1 solution in methanol and working dilution by deionized water),AFB1-BSA antigen (extent of labeling 8-12 mol Aflatoxin B1 per mol BSA), monoclonal anti-AFB1 antibody, (6 mg•mL -1 solution and working dilution by phosphate buffer solution) was obtained from Beijing Mozhidong Bio-tech (city. Country). Hydrated rare earth nitrate (RECl3•xH2O, RE Y, Yb, Er, ≥ 99.99%), oleic acid (≥ 90%) and octadecanoic acid (≥ 90%) were purchased from Sigma-Aldrich (Shanghai, China). In addition, FeCl3•6H2O, sodium fluoride, sodium hydroxide, methyl alcohol, toluene, ethyl alcohol, sodium citrate, 1,6-hexanediamine, anhydrous sodium acetate, glycol, bovine serum albumin (BSA, 96-99%),25% glutaraldehyde, tetraethyl orthosilicate (TEOS ≥ 98%), and 3-aminopropyltrimethoxysilane (APTES) was all purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the chemicals used were of analytical grade. The water used was deionized.
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8

Synthesis and Characterization of Salbutamol

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Ethyleneglycol, anhydrous ethanol, sodium acetate anhydrous, iron(iii) chloride hexahydrate, tetraethyl orthosilicate (TEOS), concentrated ammonium aqueous solution (25 wt%), sodium citrate, silver nitrate, sodium nitrite, hydrochloric acid, sodium carbonate, acetonitrile, potassium chloride and sodium chloride were purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Salbutamol (SAL), sulfanilic acid, 2,2′-benzidinedisulfonic acid, clenbuterol hydrochloride, l-lysine monohydrate, aminoacetic acid, glycerol and phosphate-buffered saline (PBS, pH 7.4) contained NaCl (100 mM) and 10 mM Na2HPO4/KH2PO4, were bought from Aladdin Reagent Co. Ltd. (Shanghai, China). Deionized water (Millipore) with a resistivity of 18 MΩ cm was used in all experiments.
2.39 mg SAL was dissolved in 10 mL methanol (1.0 × 10−3 M) and kept in the dark at 4 °C as stock solution. Then its standard solution was obtained by diluting the stock solution to the desired concentration with distilled water.
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9

Synthesis of Multifunctional Nanomaterials

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Ethanol, ethylene glycol (EG), ammonia solution, crystal violet, and PATP were purchased from Macklin Biochemical Co. Tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES) were acquired from Sigma-Aldrich Chemicals Co. Sodium acetate anhydrous and trisodium citrate dehydrate were obtained from Sinopharm Chemical Co. Ferric chloride hexahydrate (FeCl3·6H2O) and thiram were purchased from Tianjin ZhiYuan Reagent Co. Ascorbic acid, cetyltrimethylammonium bromide (CTAB), and gold chloride trihydrate (HAuCl4·3H2O) were purchased from Shanghai Aladdin Biochemical Co. DI water was obtained from a Milli-Q system.
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10

Comprehensive Wood Characterization

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Fir wood (Cunninghamia lanceolata (Lamb.) Hook, 450 ± 50 kg/m3) was selected as the representative softwood species. Poplar wood (Populus. simonii Carr, 454 ± 56 kg/m3) and balsa wood (Ochroma pyramidale, 113 ± 30 kg/m3) were chosen as the representative hardwood species. All wood species were cut into dimensions of 10 × 10 × 10 mm3 (tangential × radial × axial). Sodium chlorite (80%, NaClO2), sodium acetate anhydrous (99%, C2H3NaO2), sodium hypochlorite (Cl ≥ 5.2%, NaClO), acetic acid (99%, C2H4O2), and tert-butanol (99%, C4H10O) were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Additionally, 2,2,6,6-tetramethylpiperidinyl-1-oxide (98%, C9H18NO) was supplied by Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). It is important to note that all the chemicals used in the experiment were of analytical grade.
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