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Carrier free sodium 35s sulfate

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Carrier-free sodium [35S]sulfate is a radioactive compound used in various research applications. It contains the radioactive isotope sulfur-35, which is produced by neutron irradiation of sulphur-34. This product is supplied in an aqueous solution and can be used as a tracer or labeling agent in biochemical and biological studies.

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Lab products found in correlation

Adenosine 5 triphosphate, by Merck Group (5 mentions) Dithiothreitol (dtt), by Merck Group (4 mentions) Primestar max dna polymerase, by Takara Bio (4 mentions) Oligonucleotide primers, by Eurofins (4 mentions) Ecolume scintillation cocktail, by MP Biomedicals (4 mentions) N 2 hydroxylpiperazine n 2 ethanesulfonic acid hepes, by Merck Group (3 mentions) Trizma base, by Merck Group (3 mentions) Ultrafree mc 5000 nmwl filter units, by Merck Group (3 mentions) Qiaprep spin miniprep kit, by Qiagen (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) 3 phosphoadenosine 5 phosphosulfate, by Merck Group (2 mentions) Dpni, by New England Biolabs (2 mentions) Tapentadol, by Cayman Chemical (2 mentions) Cellulose tlc plates, by Merck Group (2 mentions) Pregnenolone, by Merck Group (2 mentions) N 2 hydroxylpiperazine n 2 ethanesulfonic acid, by Merck Group (2 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid, by Merck Group (1 mentions) 3 n morpholino propanesulfonic acid, by Merck Group (1 mentions) 2 morpholinoethanesulfonic acid, by Merck Group (1 mentions) Silica gel thin layer chromatography plates, by Merck Group (1 mentions)

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[35S] sodium sulfate (2 citations)

7 protocols using carrier free sodium 35s sulfate

1

Purification and Preparation of Bile Acid Derivatives

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5α-Cyprinol and 5β-cyprinol were generated by deconjugating their sulfate salts purified from carp gallbladder as previously described [24 (link)]. 3α,7α,12α-trihydroxy-5α-cholestanoic acid (5α-triOH CA) were prepared as previously described [25 (link)]. Dehydroepiandrosterone (DHEA), adenosine 5′-triphosphate (ATP), sodium dodecyl sulfate (SDS), dithiothreitol (DTT), dimethyl sulfoxide (DMSO), sodium acetate, 2-morpholinoethanesulfonic acid (Mes), 3-(N-morpholino)propanesulfonic acid (Mops), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes), 3-([1,1-dimethyl-2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid (Ampso), 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (Capso), 3-(cyclohexylamino)-1-propanesulfonic acid (Caps) were from Sigma Chemical Company. Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals. Silica gel thin-layer chromatography (TLC) plates were from Merck Millipore. All other chemicals were of the highest grade commercially available.
Kurogi K., Yoshihama M., Horton A., Schiefer I.T., Krasowski M.D., Hagey L.R., Williams F.E., Sakakibara Y., Kenmochi N., Suiko M, & Liu M.C. (2017). Identification and characterization of 5α-cyprinol-sulfating cytosolic sulfotransferases (Sults) in the Zebrafish (Danio rerio). The Journal of steroid biochemistry and molecular biology, 174, 120-127.
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2

Synthesis and Analysis of PAP[35S]

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E2 and 4OH-tamoxifen were products of Cayman Chemical Company (Ann Arbor, MI, USA). Diethylstilbestrol, adenosine 5’-triphosphate (ATP), dimethyl sulfoxide (DMSO), dithiothreitol (DTT), 3’-phosphoadenosine-5’-phosphosulfate (PAPS), isopropyl-1-thio-β-D-galactopyranoside (IPTG), and N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES) were from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Ecolume scintillation cocktail was obtained from MP Biomedicals (Solon, OH, USA). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Recombinant human bifunctional ATP sulfulyase/adenosine 5’-phosphosulfate kinase was prepared as previously described [34 (link)], and used to synthesize PAP[35S] from ATP and [35S]sulfate based on an established procedure [34 (link)]. Cellulose thin-layer chromatography (TLC) plates were products of Macherey-Nagel (Düren, Germany). Dpn I was purchased from New England BioLabs (Ipswich, MA, USA). PrimeStar Max DNA polymerase was a product of Takara Bio Inc. (Mountain View, CA, USA). All blue prestained protein markers were obtained from BioLand Scientific LLC. (Paramount, CA, USA). Oligonucleotide primers were synthesized by Eurofins Genomics (Louisville, KY, USA). All other reagents and chemicals used were of the highest grades commercially available.
El Daibani A.A., Alherz F.A., Abunnaja M.S., Bairam A.F., Rasool M.I., Kurogi K, & Liu M.C. (2021). Impact of Human SULT1E1 Polymorphisms on the Sulfation of 17β-Estradiol, 4-Hydroxytamoxifen and Diethylstilbestrol by SULT1E1 Allozymes. European journal of drug metabolism and pharmacokinetics, 46(1), 105-118.
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3

In Vitro Sulfation Assay for Xenobiotics

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6-OH-Mel, NAS and 4-OH-Ram were products of Toronto Research Chemicals (Toronto, Ontario, Canada). Adenosine 5’-triphosphate (ATP), 3’-phosphoadenosine 5’-phosphosulfate (PAPS), 2-morpholinoethanesulfonic acid (MES), N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES), 3-N-tris-hydroxymethyl methylamino-propanesulfonic acid (TAPS), 2-cyclohexylamino ethanesulfonic acid (CHES), 3-cyclohexylamino-1-propanesulfonic acid (CAPS), Trizma base, dithiothreitol (DTT), minimum essential medium (MEM), fetal bovine serum (FBS), penicillin G, streptomycin sulfate, and silica gel thin-layer chromatography (TLC) plates were from Sigma Chemical Company (St. Louis, MO, USA). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); and Ecolume scintillation cocktail was from MP Biomedicals (Irvine, CA, USA). Ultra free-MC 5000 NMWL filter units were products of Millipore Corporation (Bedford, MA, USA). HepG2 human hepatoma cells (ATCC HB-8065) and Caco-2 human colon adenocarcinoma cells (ATCC HTB-37) were obtained from American Type Culture Collection (Manassas, VA, USA). Pooled human lung, liver, small intestine, and kidney cytosols were purchased from XenoTech, LLC (Lenexa, KS, USA). All other chemicals used were of the highest grade commercially available.
Luo L., Zhou C., Kurogi K., Sakakibara Y., Suiko M, & Liu M.C. (2015). Sulfation of 6-hydroxymelatonin, N-acetylserotonin and 4-hydroxyramelteon by the human cytosolic sulfotransferases (SULTs). Xenobiotica; the fate of foreign compounds in biological systems, 46(7), 612-619.
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4

Enzymatic Sulfation: Protocols and Reagents

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Acetaminophen (≥ 99% in purity), 4-nitrophenol (4NP) (≥ 99% in purity), dimethyl sulfoxide (DMSO), isopropyl β-D-1-thiogalactopyranoside (IPTG), adenosine 5′-triphosphate (ATP), N-2-hydroxylpiperazine-N′-2-ethanesulfonic acid (HEPES), and dithiothreitol (DTT) were products of Sigma-Aldrich (St. Louis, MO, USA). Tapentadol (≥ 98% in purity) was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). O-DMN (≥ 98% in purity) was a product of Toronto Research Chemicals (Toronto, ON, Canada). Carrier-free sodium [35S] sulfate was from American Radiolabeled Chemicals (St. Louis, MO, USA). Recombinant human bifunctional PAPS synthase was prepared as described previously [27 (link)]. Polygram® Cellulose 300 thin-layer chromatography (TLC) plates were from Macherey-Nagel GmbH and Co. KG (Düren, Germany). PrimeSTAR® Max DNA polymerase was a product of Takara Bio (Mountain View, CA, USA). Protein molecular weight markers were from Bioland Scientific LLC (Paramount, CA, USA). Ecolume scintillation cocktail was purchased from MP Biomedicals, LLC (Irvine, CA, USA). X-Ray films were products of FUJIFILM Corporation (Tokyo, Japan). Oligonucleotide primers were custom synthesized by Eurofins Genomics (Louisville, KY, USA). QIAprep® Spin Miniprep Kit was a product of QIAGEN (Düren, Germany). All other chemicals were of the highest standards commercially available.
Rasool M.I., Bairam A.F., Gohal S.A., El Daibani A.A., Alherz F.A., Abunnaja M.S., Alatwi E.S., Kurogi K, & Liu M.C. (2018). Effects of the Human SULT1A1 Polymorphisms on the Sulfation of Acetaminophen, O-Desmethylnaproxen, and Tapentadol. Pharmacological reports : PR, 71(2), 257-265.
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5

Radiolabeled PAPS Synthesis Protocol

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Pregnenolone, DHEA, adenosine 5′-triphosphate (ATP), dimethyl sulfoxide (DMSO), N-2-hydroxylpiperazine-N′-2-ethanesulfonic acid (HEPES), dithiothreitol (DTT), and Trizma base, were products of Sigma-Aldrich (St. Louis, MO, USA). PrimeSTAR® Max DNA polymerase was a product of Takara Bio (Mountain View, CA, USA). Oligonucleotide primers were synthesized by Eurofins Genomics (Louisville, KY, USA). Cellulose TLC plates and Ultrafree-MC 5000 NMWL filter units were from EMD Millipore (Billerica, MA, USA). Ecolume liquid scintillation cocktail was a product of MP Biomedicals, LLC. (Irvine, CA, USA). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals (St. Louis, MO, USA). PAP[35S] was synthesized using recombinant human bifunctional PAPS synthase based on a previously established procedure (Yanagisawa et al., 1998 (link)). All other chemicals were of the highest grade commercially available.
Alherz F.A., El Daibani A.A., Abunnaja M.S., Bairam A.F., Rasool M.I., Sakakibara Y., Suiko M., Kurogi K, & Liu M.C. (2019). Effect of SULT2B1 Genetic Polymorphisms on the Sulfation of Dehydroepiandrosterone and Pregnenolone by SULT2B1b Allozymes. Molecular and cellular endocrinology, 496, 110535.
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6

Biochemical Characterization of Sulfotransferase Assay

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APAP, adenosine 5’-triphosphate (ATP), dimethyl sulfoxide (DMSO), dithiothreitol (DTT), and N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES) were products of Sigma Chemical Company. Morphine, tapentadol, and O-DMT were from Cayman Chemical. Cellulose thin-layer chromatography (TLC) plates were from Merck (EMD Millipore Corporation). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals. 3’-Phosphoadenosine-5’-phospho[35S]sulfate (PAP[35S]) was synthesized using ATP and carrier-free [35S]sulfate according to a previously established protocol [27 (link)]. X-Ray films were from Research Products International Corporation. Prime STAR® GXL DNA Polymerase was a product of Clontech Laboratories, Inc. Protein molecular weight markers were from Bioland Scientific LLC. PCR kit was a product of G-Biosciences. QIAprep® Spin Miniprep Kit was from QIAGEN. Ecolume scintillation cocktail was from MP Biomedical LLC. Glutathione SepharoseTM was a product of GE Healthcare Bio-Sciences. All other chemicals were of the highest grades commercially available.
Bairam A.F., Rasool M.I., Alherz F.A., Abunnaja M.S., El Daibani A.A., Kurogi K, & Liu M.C. (2018). Effects of Human SULT1A3/SULT1A4 Genetic Polymorphisms on the Sulfation of Acetaminophen and Opioid Drugs by the Cytosolic Sulfotransferase SULT1A3. Archives of biochemistry and biophysics, 648, 44-52.
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7

Sulfotransferase Activity Assay

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Pregnenolone, adenosine 5′-triphosphate (ATP), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Trizma base, and dithiothreitol (DTT) were from Sigma-Aldrich (St. Louis, MO, USA). PrimeSTAR® Max DNA polymerase was a product of Takara Bio (Mountain View, CA, USA). Oligonucleotide primers were synthesized by Eurofins Genomics (Louisville, KY, USA). Dpn I and the DNA ladder were products of New England Biolabs (Ipswich, MA, USA). QIAprep® Spin Miniprep Kit was from QIAGEN (Germantown, MD, USA). Glutathione Sepharose was a product of GE Healthcare Bio-Sciences (Pittsburgh, PA, USA). Cellulose TLC plates and Ultrafree-MC 5000 NMWL filter units were from EMD Millipore (Billerica, MA, USA). X-ray films were sourced from Products International Corporation (Mt Prospect, IL, USA). Ecolume scintillation cocktail was purchased from MP Biomedicals, LLC. (Solon, OH, USA). Carrier-free sodium [35S] sulfate was from American Radiolabeled Chemicals (St. Louis, MO, USA). [35S]PAPS was synthesized from adenosine triphosphate (ATP) and carrier-free [35S]sulfate using recombinant human bifunctional ATP sulfurylase/ adenosine 5’-phosphosulfate (APS) kinase. The [35S]PAPS synthesized was adjusted to the required concentration and specific activity of 15 Ci/mmol at 1.4 mM by the addition of unlabeled PAPS17 (link). All other chemicals were of the highest grade commercially available.
Alatwi E, & Bairam A.F. (2024). The role of genetic polymorphisms in the sulfation of pregnenolone by human cytosolic sulfotransferase SULT2B1a. Scientific Reports, 14, 8050.
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