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Microscopic glass slides

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Microscopic glass slides are flat, transparent surfaces used for the observation and analysis of microscopic specimens. They provide a stable and standardized platform to mount and secure samples for examination under a microscope.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Phenformin, by Merck Group (1 mentions) Vectashield hardset antifade mounting medium with dapi h 1500, by Vector Laboratories (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Superscript vilo master mix, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions) Metformin, by Merck Group (1 mentions) Hiperfect transfection reagent, by Qiagen (1 mentions) Micro cover glass, by Avantor (1 mentions) Milli q advantage a10 water purification system, by Merck Group (1 mentions) Amplex ultrared reagent, by Thermo Fisher Scientific (1 mentions) N n bis acryloyl cystamine bac, by Thermo Fisher Scientific (1 mentions) Rsa g2 solids analyzer, by TA Instruments (1 mentions) Polyethylene glycol diacrylate pegda mw 400, by Polysciences (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Axiocam 105, by Zeiss (1 mentions) Stemi 508 stereo microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Microscopic slide (2 citations) Glass slides (68 citations)

5 protocols using microscopic glass slides

1

Optimizing Cell Culture Reagents and Tools

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Methanol and ethanol were from VWR International Ltd. (Poole, UK). Metformin (D150959), phenformin (p7045), and other general chemicals were purchased from Sigma-Aldrich (Poole, UK). ECL Western blotting analysis system (RNP2109) was from GE Healthcare (Little Chalfont Bucks). Dulbecco’s Modified Eagle Medium (DMEM) (41965–039, 31885–023), Dulbecco’s phosphate-buffered saline (DPBS) (14190), trypsin (25300–054), penicillin-streptomycin (15140122), lipofectamine 2000, Superscript VILO Master Mix (11755–050), Taqman Universal PCR Master Mix (4324020) were from Thermo-Fisher Scientific (Perth, Scotland). HiPerFect transfection reagent (301704) was from Qiagen (Manchester, UK). Microscopic glass slides (631–0906) were from VWR International (Lutterworth, UK). Vectashield hard-set anti-fade mounting medium with DAPI (H-1500) was from Vector Laboratories Inc. (Peterborough, UK). Details of antibodies (S1 Table), plasmids (S2 Table, oligos (S3 Table) and viruses (S2 Table) are listed in S1–S3 Tables.
Chen C., Gallagher J.R., Tarlton J., van Aalten L., Bray S.E., Ashford M.L., McCrimmon R.J., Pearson E.R., McNeilly A.D, & Sutherland C. (2021). The genetic association of the transcription factor NPAT with glycemic response to metformin involves regulation of fuel selection. PLoS ONE, 16(7), e0253533.
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2

Optical Thin Film Characterization

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The substrates were chosen according to the measurements performed afterwards. Indeed, spectrophotometer measurements require a transparent substrate, therefore microscopic glass slides (VWR international) were used. Spectroscopic ellipsometry required a high optical index difference between the substrate and the thin film layer, thus a silicon wafer was chosen. The substrates were cleaned in an ultrasonic bath in acetone and ethanol, dried by nitrogen flow and an oxygen plasma. The latter step also increased the wettability of the substrate.
The deposition of the thin film layers was performed by spin coating. The spin coating speed was set to 500–1000 rpm, in order to obtain layers of a few hundred micrometers thickness. The deposition technique yields homogeneous thin film layers in the center of the sample where the optical measurements are performed. Nevertheless, every measurement was repeated at least twice on two different areas to avoid thickness-dependent artefacts. The thickness and homogeneity of the thin films were determined by a mechanical stylus profilometer (Brucker Decktak XT).
Carlberg M., Pourcin F., Margeat O., Le Rouzo J., Berginc G., Sauvage R.M., Ackermann J, & Escoubas L. (2017). Optical response of heterogeneous polymer layers containing silver nanostructures. Beilstein Journal of Nanotechnology, 8, 1065-1072.
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3

Hydrogel-based Enzyme Detection System

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Acrylamide (AAm) and N,N′-bis(acryloyl)cystamine (BAC) were purchased from Alfa Aesar. Tetramethylethylenediamine (TEMED) and Amplex UltraRed reagent (AUR) were purchased from ThermoFisher Scientific. Peroxidase from horseradish (HRP), 3-(trimethoxysilyl) propyl methacrylate, dextran from Leuconostoc spp (Mr ~ 100000), and anhydrous (≥99.9%) dimethyl sulfoxide were purchased from Sigma-Aldrich. Ammonium persulfate (APS) was purchased from CalabrioChem Inc. Polyethylene(glycol) diacrylate (PEGDA; Mw = 400) was purchased from PolySciences Inc. Ethanol (EtOH) was purchased from Decon Laboratories Inc. Glacial acetic acid was purchased from Macron Fine Chemicals. Water (dd-H2O) was ionized using Milli-Q Advantage A-10 water purification system (Millipore, U.S.A.). Microscopic glass slides (3” × 1” × 1.2 mm) and micro cover glass (18 mm × 18 mm) were purchased from VWR. Rain-X water repellant manufactured by ITW (Global Bands, TX) was purchased from Home Depot. All UV−vis absorbance spectra were acquired on a Safire2 UV−vis microplate reader (TECAN, Switzerland). The absorbance for the samples were measured at 568 nm in a Nunclon 96-clear Microwell plate. Mechanical characterization tests were performed on a TA Instruments RSA-G2 Solids Analyzer.
Parameswar A.V., Fitch K.R., Bull D.S., Duke V.R, & Goodwin A.P. (2018). Polyacrylamide Hydrogels Produce Hydrogen Peroxide from Osmotic Swelling in Aqueous Media. Biomacromolecules, 19(8), 3421-3426.
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4

Measuring Bumblebee Wing Dimensions

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After cuticle surface extraction, the forewings of each individual were cut off and mounted on microscopic glass slides (76 × 26 mm, VWR International, Radnor, USA). A second glass slide was placed on top of the wings, which were thus flattened, in order to improve the quality of the subsequently taken photographs. We used an Axiocam 105 color microscope camera (Zeiss, Germany) mounted on a Stemi 508 stereo microscope (Zeiss, Germany) to photograph each individual wing. For photography, we employed the transmitting light source of the stereo microscope in order to avoid any reflections on the wings. As a proxy for size, we measured the distance from the proximal end of the first cubital cell to the distal end of the third cubital cell [53 ] by means of the analytical software ZEN 3.2 (blue edition, Zeiss). This size was also used to normalize the absolute scent amount on the surface of each individual bumblebee.
Straub F., Kuppler J., Fellendorf M., Teuscher M., Vogt J, & Ayasse M. (2022). Land-use stress alters cuticular chemical surface profile and morphology in the bumble bee Bombus lapidarius. PLoS ONE, 17(5), e0268474.
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5

Fabrication of PEG-DA Hydrogel Scaffolds

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Poly(ethylene glycol) diacrylate (PEG-DA) (MW 575), 2-hydroxy-2-methylpropriophenone (HMPP), 3-(trichlorosilyl) propyl methacrylate (TPM), hexamethyldisilazane, and fibronectin were purchased from Sigma-Aldrich (St. Louis, MO). AZ P4620 photoresist and AZ 400K developer were obtained from AZ Electronic Materials Corp. (Somerville, NJ). Hexane, dichloromethane, ethanol and acetone were purchased from PHARMCO-AAPER (Brookfield, CT). A Sylgard 184 Silicone Elastomer Kit containing polydimethylsilane (PDMS) elastomer and curing agent was purchased from Dow Corning Corporation (Midland, MI). Calcein-AM was obtained from Molecular Probes-Life Technologies (Grand Island, NY). Microscopic glass slides were purchased from VWR (Batavia, IL). The photomask was obtained from CAD/Art Services, Inc. (Bandon, OR).
Brown C.L., Fleischauer V, & Heo J. (2017). High-throughput Screening of Erratic Cell Volume Regulation Using a Hydrogel-based Single-cell Microwell Array. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(4).
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