Phistone h3

Manufactured by Abcam

Histone H3 is a core histone protein that forms part of the nucleosome in eukaryotic chromatin. It plays a crucial role in the structural organization of DNA within the cell nucleus.

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Lab products found in correlation

3 protocols using phistone h3

Multimarker Immunohistochemistry for Mouse Brain

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Mouse: Sox2 (Santa Cruz, sc-365823, 1:500), Hopx (Santa Cruz, sc-398703, 1:250), Cofilin (Santa Cruz, sc-376476, 1:100), Cdc42 (Santa Cruz, sc-8401, 1:200) Cux1 (Abcam, AB54583, 1:500), pVim (Fisher, 5045934, 1:500), pHistone H3 (abcam, ab14955, 1:500), Cryab (ab13496, 1:500), Rabbit: Hopx (Proteintech, 11419–1-AP, 1:500), pS6 (Cell Signaling, 2211S, 1:500), p4EBP1 (Cell Signaling Technology, 2855S, 1:500), DEPDC5 (Sigma, HPA054969, 1:500), Arp2 (Proteintech, 10922–1-AP, 1:100), Cleaved Caspase-3 (9661S, 1:200). Rat: Ctip2 (Abcam, ab18465, 1:500), BrdU (Abcam, ab6326, 1:500). Sheep: Eomes (R and D, AF6166, 1:200), Goat: Sox2 (Santa Cruz, sc-17320, 1:250), Guinea pig: NeuN (Millipore, ABN90, 1:500), Chicken: Gfp (Aves, GFP-1020, 1:500).

Andrews M.G., Subramanian L, & Kriegstein A.R. (2020). mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex. eLife, 9, e58737.

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Immunostaining of Cultured Cells and Pancreatic Tissue

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Methods for these procedures in cultured cells and pancreatic tissue are as previously described (McKimpson et al., 2013 (link)). Antibodies included ARC (Cayman Chemical), insulin (Abcam), insulin (Dako), cleaved caspase-3 (Cell Signaling Technology), pHistone H3 (Abcam), Myc-Tab (Cell Signaling), and AAT (provided by E. Miranda, Sapienza University of Rome and J. Perez, University of Malaga). Secondary antibodies included Alexa Fluor 488, 568, and 647 (Invitrogen) for immunostaining. Cover slips were applied using VECTASHIELD Mounting Medium with DAPI (Vector Laboratories) to counterstain nuclei. Images were collected with an Axio Observer.Z1 microscope (Zeiss). Confocal images were collected with a Zeiss LSM 510/710 confocal microscope (Zeiss).

McKimpson W.M., Chen Y., Irving J.A., Zheng M., Weinberger J., Lek Wen Tan W., Tiang Z., Jagger A.M., Chua SC J.r., Pessin J.E., Foo R.S., Lomas D.A, & Kitsis R.N. (2021). Conversion of the death inhibitor ARC to a killer activates pancreatic β-cell death in diabetes. Developmental cell, 56(6), 747-760.e6.

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Western Blot Analysis of Protein Expression

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Samples were separated on a 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto nitrocellulose membranes and then blocked in 10% dry milk‐TBST (20 mmol/L Tris‐HCl [PH 7.6], 127 mmol/L NaCl, 0.1% Tween‐20) for two hours at 37°C. Following three washes in Tris‐HCl pH 7.5 with 0.1% Tween 20, the blots were incubated with primary antibody overnight at 4°C. Following three washes, membranes were then incubated with secondary antibody overnight at 4°C. Primary antibodies include the following: anti‐glypican‐3 (Abcam), anti‐PCNA (Abcam), anti‐METTL3 (Abcam), anti‐histone H3 (Abcam), anti‐H3K9me3 (Abcam), anti‐JMJD2A (Santa Cruz, Biotech), anti‐Pim1 (Santa Cruz, Biotech), pHistone H3 (Abcam), anti‐SUZ12 (Santa Cruz, Biotech), anti‐SUV39H1 (Santa Cruz, Biotech), anti‐Nanog (Abcam), anti‐MEKK4 (Abcam), anti‐pTyr (Abcam). Signals were visualized by enhanced chemiluminescence plus kit (GE Healthcare) according to our pervious protocol.34

Yang Y., Song S., Meng Q., Wang L., Li X., Xie S., Chen Y., Jiang X., Wang C., Lu Y., Xin X., Pu H., Gui X., Li T., Xu J., Li J., Jia S, & Lu D. (2020). miR24‐2 accelerates progression of liver cancer cells by activating Pim1 through tri‐methylation of Histone H3 on the ninth lysine. Journal of Cellular and Molecular Medicine, 24(5), 2772-2790.

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