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Goat anti chicken alexa 594

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Goat anti-chicken Alexa-594 is a secondary antibody conjugated with the Alexa Fluor 594 dye. It is used to detect and visualize chicken-derived primary antibodies in immunofluorescence and other immunoassay applications.

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Lab products found in correlation

Goat anti rabbit alexa 594, by Thermo Fisher Scientific (2 mentions) Axioplan 2 fluorescence microscope, by Zeiss (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Goat anti mouse alexa 488, by Thermo Fisher Scientific (2 mentions) Goat anti chicken alexa 488, by Thermo Fisher Scientific (2 mentions) Rabbit anti brn3, by Santa Cruz Biotechnology (2 mentions) Chicken anti rabbit alexa 594, by Thermo Fisher Scientific (2 mentions) Goat anti mouse alexa 594, by Thermo Fisher Scientific (2 mentions) Chicken anti β gal, by Abcam (2 mentions) Prolong gold, by Thermo Fisher Scientific (2 mentions) Rabbit anti sox2, by Abcam (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Rabbit anti β tubulin 3, by Fortrea (2 mentions) Bx61 microscope, by Olympus (2 mentions) Sp5 2 microscope, by Leica (2 mentions) Goat anti rabbit alexa 488, by Thermo Fisher Scientific (1 mentions) Orca er ccd camera, by Hamamatsu Photonics (1 mentions) Axioplan 2, by Zeiss (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Neurobasal, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Alexa 594 (538 citations) Goat anti-chicken (Alexa Fluor 594, (10 citations) Alexa Fluor 594 chicken anti-goat IgG (7 citations) Goat anti-chicken 594 (3 citations) Goat anti-chicken IgG-conjugated Alexa Fluor 594 (2 citations) Alexa Fluor 594 Goat anti-chicken IgY secondary antibody (2 citations)

5 protocols using goat anti chicken alexa 594

1

Characterization of Complement Signaling in VEGF Tumor Cells

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ID8-VEGF tumor cells were stably transduced with murine C3 shRNA, C3aR shRNA, C5aR shRNA, or C5L2 shRNA carrying lentiviri and plated at a density of 5 × 103 cells in a 2-well chamber slide. Twenty-four hours after plating, tumor cells were fixed with 4% paraformaldehyde for 30 min and permeabilized in 0.01% Triton X-100 in PBS for 10 min. Following incubation with the primary antibodies (C3, C3aR, C5aR, and C5L2, 1:100 diluted in 1% BSA-PBS) for 1 hr at room temperature, cells were incubated with the secondary antibodies (goat anti-rabbit Alexa 594, 1:1,000 diluted; chicken anti-goat Alexa 594, 1:1,000 diluted) (Life Technologies) for 1 hr at room temperature. DAPI (Life Technologies) was used as a nuclear counterstain. Images were obtained using the Zeiss Axioplan 2 Fluorescence microscope.
Cho M.S., Vasquez H.G., Rupaimoole R., Pradeep S., Wu S., Zand B., Han H.D., Rodriguez-Aguayo C., Bottsford-Miller J., Huang J., Miyake T., Choi H.J., Dalton H.J., Ivan C., Baggerly K., Lopez-Berestein G., Sood A.K, & Afshar-Kharghan V. (2014). Autocrine Effects of Tumor-Derived Complement. Cell reports, 6(6), 1085-1095.
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2

Characterization of Complement Signaling in VEGF Tumor Cells

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ID8-VEGF tumor cells were stably transduced with murine C3 shRNA, C3aR shRNA, C5aR shRNA, or C5L2 shRNA carrying lentiviri and plated at a density of 5 × 103 cells in a 2-well chamber slide. Twenty-four hours after plating, tumor cells were fixed with 4% paraformaldehyde for 30 min and permeabilized in 0.01% Triton X-100 in PBS for 10 min. Following incubation with the primary antibodies (C3, C3aR, C5aR, and C5L2, 1:100 diluted in 1% BSA-PBS) for 1 hr at room temperature, cells were incubated with the secondary antibodies (goat anti-rabbit Alexa 594, 1:1,000 diluted; chicken anti-goat Alexa 594, 1:1,000 diluted) (Life Technologies) for 1 hr at room temperature. DAPI (Life Technologies) was used as a nuclear counterstain. Images were obtained using the Zeiss Axioplan 2 Fluorescence microscope.
Cho M.S., Vasquez H.G., Rupaimoole R., Pradeep S., Wu S., Zand B., Han H.D., Rodriguez-Aguayo C., Bottsford-Miller J., Huang J., Miyake T., Choi H.J., Dalton H.J., Ivan C., Baggerly K., Lopez-Berestein G., Sood A.K, & Afshar-Kharghan V. (2014). Autocrine Effects of Tumor-Derived Complement. Cell reports, 6(6), 1085-1095.
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3

Immunofluorescence Staining of Cryosectioned Eyes

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Eyes were harvested and fixed in 4% PFA for 2 hours prior to cryoprotection in 25% sucrose-PBS overnight. Eyes were embedded in OCT, and cryosectioned at 12 μm and mounted on SuperFrost Plus slides prior to immunofluorescence. Standard immunofluorescence procedures were followed.9 (link) Primary antibodies include: chicken anti-β-gal (Abcam Cambridge, MA; ab9361), rabbit anti-β-tubulin III (Covance, Princeton, NJ; PRB-435P), rabbit anti-Brn3 (Santa Cruz Biotechnology, Santa Cruz, CA; sc28595), and rabbit anti-Sox2 (Abcam; ab97959). Secondary antibodies used were (all from Invitrogen): chicken anti-rabbit Alexa-594, goat anti-chicken Alexa-488, goat anti-chicken Alexa-594, goat anti-mouse Alexa-488, goat anti-mouse Alexa-594, goat anti-rabbit Alexa-488, and goat anti-rabbit Alexa-594. Nuclei were stained using 1 μg/ml of Hoechst 33342 (Sigma, St Louis, MO; 14533). Slides were mounted with Prolong Gold (Invitrogen) antifade reagent and imaged using an Olympus BX61 microscope and the InVivo software (Olympus), or alternatively using confocal laser scanning microscopy with a Leica SP5 II microscope (Leica Microsystems) and LAF software (Leica Microsystems).
de Leeuw C.N., Dyka F.M., Boye S.L., Laprise S., Zhou M., Chou A.Y., Borretta L., McInerny S.C., Banks K.G., Portales-Casamar E., Swanson M.I., D’Souza C.A., Boye S.E., Jones S.J., Holt R.A., Goldowitz D., Hauswirth W.W., Wasserman W.W, & Simpson E.M. (2014). Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Molecular Therapy. Methods & Clinical Development, 1, 5-.
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4

Immunohistochemistry of Retinal Tissues

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Eyes were harvested and fixed in 4% PFA for 2 hours prior to cryoprotection in 25% sucrose-PBS overnight. Eyes were embedded in OCT, and cryosectioned at 12 μm and mounted on SuperFrost Plus slides prior to immunofluorescence. Standard immunofluorescence procedures were followed.9 (link) Primary antibodies include: chicken anti-β-gal (Abcam Cambridge, MA, ab9361), rabbit anti-β-tubulin III (Covance, Princeton, NJ, PRB-435P), rabbit anti-Brn3 (Santa Cruz Biotechnology, Santa Cruz, CA, sc28595), and rabbit anti-Sox2 (Abcam, ab97959). Secondary antibodies used were (Invitrogen): chicken anti-rabbit Alexa-594, goat anti-chicken Alexa-488, goat anti-chicken Alexa-594, goat anti-mouse Alexa-488, goat anti-mouse Alexa-594, goat anti-rabbit Alexa-488, goat anti-rabbit Alexa-594. Nuclei were stained using 1 μg/mL of Hoechst 33342 (Sigma, St. Louis, MO, 14533). Slides were mounted with Prolong Gold (Invitrogen) anti-fade reagent and imaged using an Olympus BX61 microscope and the InVivo software, or alternatively using confocal laser scanning microscopy with a Leica SP5 II microscope and LAF software.
de Leeuw C.N., Dyka F.M., Boye S.L., Laprise S., Zhou M., Chou A.Y., Borretta L., McInerny S.C., Banks K.G., Portales-Casamar E., Swanson M.I., D’Souza C.A., Boye S.E., Jones S.J., Holt R.A., Goldowitz D., Hauswirth W.W., Wasserman W.W, & Simpson E.M. (2014). Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Molecular Therapy. Methods & Clinical Development, 1, 5-.
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5

Immunofluorescence Imaging of Cultured Neurons

Cited 3 times
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aiFACS-sorted neurons were plated on ornithine-coated glass coverslips (35-mm diameter) and cultivated in complete medium: neurobasal (Invitrogen) supplemented with B-27 (Invitrogen) and GlutaMAX (Invitrogen) as previously described (Abekhoukh et al. 2017 (link); Maurin et al. 2019 (link)). Neurons were fixed, and immunofluorescence was performed with microtubule associated protein 2 (MAP2) antichicken polyclonal antibody (BioLegend 822501) detected with a secondary goat antichicken Alexa 594 (Invitrogen A32759) 6 d after the selection, as previously described (Drozd et al. 2019 (link)). Fluorescent images were taken using a wide-field upright fluorescence microscope (Axioplan2, Carl Zeiss), with an ORCA ER CCD camera (Hamamatsu), through a rhodamine filter set (BP565/30; LP585; BP620/60) and a PlanApoChormat 63×/1.4 DIC oil immersion objective (pixel size: 100 nm).
Microglia were labeled as previously described (Cazareth et al. 2014 (link)) using the following antibodies: BV510 anti-mouse CD45-clone 30-F11 (BD Biosciences 563891) and AlexaFluor700 anti-mouse CD11b-clone M1/70 (Sony Biotechnology 1106110).
Castagnola S., Cazareth J., Lebrigand K., Jarjat M., Magnone V., Delhaye S., Brau F., Bardoni B, & Maurin T. (2020). Agonist-induced functional analysis and cell sorting associated with single-cell transcriptomics characterizes cell subtypes in normal and pathological brain. Genome Research, 30(11), 1633-1642.
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