Oci ly3

293T and Burkitt's lymphoma cell lines (Raji, Daudi and Namalwa) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China); Ramos (Burkitt's lymphoma cell line) and Pfeiffer (DLBCL cell line) cell lines were purchased from the ATCC; and OCI-ly3 (DLBCL cell line) and OCI-ly6 (DLBCL cell line) cell lines were gifted by Dr. X. Jiang (China) and Professor T. Zhao (China), respectively. The 293T cell line was maintained in Dulbecco's modified Eagle's medium with 10% FBS (Gibco). Most lymphoma cell lines were maintained in Roswell Park Memorial Institute-1640 (RPMI-1640) with 10% FBS (Gibco), and the OCI-ly3 and the OCI-ly6 cell lines were maintained in ISCOVE's modified DMEM (IMDM) with 10% FBS (Gibco).

Human normal B-cell line HMy2.CIR was preserved in our laboratory and DLBCL TMD8, OCI-Ly3, U2932, OCI-Ly1, OCI-Ly7, SU-DHL-4, and SU-DHL-6 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HMy2.CIR, OCI-Ly3, and OCI-Ly7 cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) (Gibco, USA) containing 10% fetal bovine serum (FBS), 100 μg mL⁻¹ streptomycin, and 100 U mL⁻¹ penicillin (HyClone). The rest of the cells were maintained in RPMI 1640 medium with 10% FBS. All cells were cultivated in a humidified incubator at 37℃ with 5% CO₂. To knockdown HK2 in the cells, siRNA sequences against HK2 (si-HK2 #1: 5′-GGAGATGGAGAAAGGGCTT-3′; si-HK2 #2: 5′-GCGCATCAAGGAGAACAAA-3′) or negative control (si-NC: 5′-AGACTTCCTCACCTCCTTCT-3′) were synthesized by Genepharm Co. Ltd. (Shanghai, China). All transfections were performed using Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer’s procedures. The transfection efficiency was assessed with quantitative reverse transcription polymerase chain reaction (qRT-PCR).