Transwell filter units

Manufactured by Corning

Sourced in United States

Transwell filter units are a laboratory equipment designed for cell culture studies. They consist of a permeable membrane insert that fits into a well of a culture plate, allowing for the separation and study of different cell populations or the transport of molecules across the membrane.

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Lab products found in correlation

Epithelial volt ohm meter, by Merck Group (1 mentions)

3 protocols using transwell filter units

Chondrogenic Differentiation of Human Mesenchymal Stem Cells

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Human bone marrow mesenchymal stem cells were isolated by adherence to tissue culture plastic from the mononuclear cell fraction of human bone marrow (Stem Cell Technologies, Cambridge, UK) and expanded in monolayer culture in MSC Growth Medium supplemented with FGF2 [31 (link)]. Cells (at passages 2–5) were dissociated with trypsin and transferred to serum-free chondrogenic medium containing TGFβ3, dexamethasone, ascorbic acid 2-phosphate, sodium pyruvate, proline and ITS +1 as previously described [32 (link)]. Aliquots of cells (5 × 10⁵) were centrifuged in 6.5 mm diameter Corning Trans-well^™ filter units. Cultures were maintained at 37 °C, 5% CO₂ for 3, 7, 14, and 28 days for cartilage formation. Growth media were replenished on a two-day interval. Cartilage constructs were frozen for future biochemical analysis.
For histology cultures were fixed in 4% paraformaldehyde and processed into paraffin wax. Sections were cut at 5 μm and distribution of polyanions revealed by staining with Safranin O/Fast Green.

Murdoch A.D., Hardingham T.E., Eyre D.R, & Fernandes R.J. (2015). The development of a mature collagen network in cartilage from human bone marrow stem cells in Transwell culture. Matrix biology : journal of the International Society for Matrix Biology, 50, 16-26.

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Transepithelial Resistance Measurement of A549 Cells

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A549 monolayer grown in Transwell filter units (3-μm-diameter pores, Corning, New York, NY, USA) were left uninfected or infected with 1 MOI of H5N1 virus and then incubated in the absence or presence of the indicated inhibitors. The monolayers were analyzed for transepithelial resistance (Epithelial Volt-‘Ohm Meter, Millipore, St. Louis, MO) at the indicated time after virus infection. The measured values were calculated by multiplying the measured electrical resistance by the area of the filter. The results represent the mean ± SD of three independent experiments.

Ruan T., Sun Y., Zhang J., Sun J., Liu W., Prinz R.A., Peng D., Liu X, & Xu X. (2022). H5N1 infection impairs the alveolar epithelial barrier through intercellular junction proteins via Itch-mediated proteasomal degradation. Communications Biology, 5, 186.

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MDCK Cells Culture and Overexpression

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Type II Madin–Darby canine kidney (MDCK) cells were used between passages 3 and 10. These cells were originally cloned by Dr. D. Louvard (European Molecular Biology Laboratory, Heidelberg, Germany) and came to us via Dr. Mostov who obtained them from Dr. K. Matlin (University of Chicago, Chicago, IL). We previously generated MDCK type II cells overexpressing myc‐tagged‐hSec10 (Lipschutz et al. 2000 (link)). MDCK type II cells overexpressing Smoothened‐YFP were generously provided to us by Drs. Ott and Lippincott‐Schwartz (Ott et al. 2012 (link)). Cells were cultured in modified Eagle's minimal essential medium containing Earl's balanced salt solution and glutamine supplemented with 5% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. MDCK cells were seeded at confluence on 24‐mm Transwell filter units coated with collagen (Corning Life Sciences, Lowell, MA). Cell monolayers were used for experiments after 7–14 days of culture with daily changes in medium.

Chacon‐Heszele M.F., Choi S.Y., Zuo X., Baek J., Ward C, & Lipschutz J.H. (2014). The exocyst and regulatory GTPases in urinary exosomes. Physiological Reports, 2(8), e12116.

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