Lamp1 clone h4a3

Manufactured by Developmental Studies Hybridoma Bank

LAMP1 clone H4A3 is a monoclonal antibody produced by the Developmental Studies Hybridoma Bank. It is directed against the Lysosome-Associated Membrane Protein 1 (LAMP1), a glycoprotein found in the membranes of lysosomes and late endosomes. The antibody can be used for the detection and localization of LAMP1 in various experimental applications.

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Lab products found in correlation

Src inhibitor pp2, by Merck Group (1 mentions)

2 protocols using lamp1 clone h4a3

Antibody Optimization for β-Catenin and LAMP1

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The following antibodies were used in this study: mouse mAb anti β-catenin (BD TL 610154, Lot #9315374, IF 1:600), mouse mAb anti LAMP1 clone H4A3 (Developmental Studies Hybridoma Bank, IF 1:600). Reagents: recombinant human vitronectin (VN, Peprotech 140-09), fibronectin (FN, isolated from human plasma, SA #F2006), rat tail type I collagen (Advanced BioMatrix #5153), methylcellulose (Sigma-Aldrich #M7027), poly(2-hydroxyethyl methacrylate (polyHEMA, Sigma-Aldrich #P3932); Src inhibitor PP2 (Sigma-Aldrich #529573); MEK1/2 inhibitor CI-1040 (Atriva Therapeutics GmbH, Tübingen, Germany, kindly provided by Dr. A. Schreiber, Institute of Molecular Virology, ZMBE, Münster).

Schwietzer M.F., Thölmann S., Kummer D., Kaschler A., Greune L., Thüring E.M., Schmidt M.A., Gerke V, & Ebnet K. (2022). Loss of contact inhibition of locomotion in the absence of JAM-A promotes entotic cell engulfment. iScience, 25(10), 105144.

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Manipulating Enteroid Polarity with Antibodies

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BME-embedded enteroids were treated with no antibody, anti-β1 integrin-neutralizing antibody clone AIIB2 (Developmental Studies Hybridoma Bank), or a control antibody against intracellular antigen LAMP-1 clone H4A3 (Developmental Studies Hybridoma Bank). Antibodies were diluted to 2.2 μg/mL in growth media and added to enteroids for 1 day. Treated enteroids were analyzed by confocal microscopy to determine the frequency of basal-out, apical-out, and mixed polarity enteroids.

Co J.Y., Margalef-Català M., Li X., Mah A.T., Kuo C.J., Monack D.M, & Amieva M.R. (2019). Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions. Cell Reports, 26(9), 2509-2520.e4.

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