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Kras sc 30

Manufactured by Santa Cruz Biotechnology
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KRAS (sc-30) is a lab equipment product from Santa Cruz Biotechnology. It is used for the detection and analysis of the KRAS protein, which is a key regulator of cellular signaling pathways. The product provides a tool for researchers to study the role of KRAS in various biological processes and disease states.

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Lab products found in correlation

Ripa buffer, by Merck Group (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) Snail, by Cell Signaling Technology (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) Vimentin, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) β actin a5441, by Merck Group (1 mentions) Protein assay, by Bio-Rad (1 mentions) C myc sc 40, by Santa Cruz Biotechnology (1 mentions) Phospho jnk1 2, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) N cadherin, by BD (1 mentions) E cadherin, by BD (1 mentions) Actin sc 1616, by Santa Cruz Biotechnology (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) H3k4me2, by Cell Signaling Technology (1 mentions)

6 protocols using kras sc 30

1

Cell Protein Extraction and Antibody Detection

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Proteins were extracted by collecting cells in RIPA buffer (Sigma-Aldrich) containing Complete Protease Inhibitor Cocktail (Roche), and protein concentration was determined using the Bio-Rad Protein Assay (Bio-Rad). Proteins were detected using the following antibodies: KRAS (sc-30) from Santa Cruz.; VEGF-A (ab46154, sc-7269) from Abcam and Santa Cruz; and β-actin (A5441) from Sigma.
Yoon C., Lu J., Jun Y., Suh Y.S., Kim B.J., Till J.E., Kim J.H., Keshavjee S.H., Ryeom S, & Yoon S.S. (2023). KRAS activation in gastric cancer stem-like cells promotes tumor angiogenesis and metastasis. BMC Cancer, 23, 690.
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2

Comprehensive Protein Detection in Biological Samples

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Samples were collected in RIPA buffer (Sigma) containing Complete Protease Inhibitor Cocktail (Roche Diagnostics), and protein concentration was determined using the Bio-Rad Protein Assay. Western blots employed the following antibodies: CDK5RAP3 (sc-271776), ERK1 (sc-271270), Kras (sc-30) and c-Myc (sc-40) purchased from Santa Cruz Biotechnology; Sox2 (#2748, #3579), Oct4 (#2750), Nanog (#4893), Slug (#9585), Snail (#3879), phospho-ERK1/2 (#9101), phospho-JNK1/2 (#9251), phospho-p38 (#9211), Ras (#3965), CD44 (#3578, #3570), E-cadherin (#14472), vimentin (#3932) and cleaved caspase-3 (#9661) from Cell Signaling; N-cadherin (BD610920) and E-cadherin (BD610181) from BD Biosciences; Zeb1 (NBP-1-05987) from Novus Biologicals; and β-actin from Sigma.
Lin J.X., Yoon C., Li P., Ryeom S.W., Cho S.J., Zheng C.H., Xie J.W., Wang J.B., Lu J., Chen Q.Y., Yoon S.S, & Huang C.M. (2020). CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer. British Journal of Cancer, 123(7), 1131-1144.
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3

Protein Extraction and Western Blot Analysis

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Cultured cells were washed with phosphate buffered saline (PBS; Gibco, USA) and lysed with lysis buffer (50 mM Tris-HCl, pH 7.5 1% NP-40, 0.1% sodium deoxycholate, 150 mM NaCl, 50 mM NaF, 1 mM sodium pyrophosphate, 1 mM EDTA and protease/phosphatase inhibitors). Protein concentrations of the cell lysates were quantified with a Bicinchoninic Acid Protein Assay Reagent (Pierce, Rockford, IL), according to the manufacturer’s instructions. Samples containing equal quantities of total proteins were resolved in SDS-polyacrylamide denaturing gel, transferred to nitrocellulose membranes. The membranes were incubated in blocking buffer containing 1% skim milk and 1% bovine serum albumin (BSA, Sigma-Aldrich, USA) for an hour at room temperature and probed overnight at 4 °C with primary antibodies. Antibodies against KRAS (#sc-30) and Actin (#sc-1616) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Lee W., Lee J.H., Jun S., Lee J.H, & Bang D. (2018). Selective targeting of KRAS oncogenic alleles by CRISPR/Cas9 inhibits proliferation of cancer cells. Scientific Reports, 8, 11879.
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4

Prostate Cancer Tissue Microarray Analysis

Cited 1 time
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A prostate cancer tissue microarray with 80 cases and Gleason grade information was purchased (PR803b, US Biomax). Archived prostate cancer FFPE specimens of adjacent normal, primary tumor and metastasis (total n = 49) were requested from MD Anderson Cancer Center Prostate Cancer SPORE program (Specialized Programs of Research Excellence) under approved IRB protocol at MD Anderson Cancer Center. For all clinical samples, written informed consent was obtained from the patients. The studies were conducted in accordance with recognized ethical guidelines (Declaration of Helsinki, CIOMS, Belmont Report, U.S. Common Rule). H&E stain, immunohistochemical (IHC) and western blot were performed as previously described (21 (link)). Primary antibodies used include PYGO2 (HPA023689, Sigma, for IHC; GTX119726, GeneTex, for western blot), KRAS (sc-30, Santa Cruz), FGFR1, β-Catenin, c-Myc, Met, H3K4me2, H3K4me3, H3 (9740, 8480, 5605, 8198, 9725, 9751 and 4499, Cell Signaling Technology), β-actin (A2228, Sigma).
Lu X., Pan X., Wu C.J., Zhao D., Feng S., Zang Y., Lee R., Khadka S., Amin S.B., Jin E.J., Shang X., Deng P., Luo Y., Morgenlander W.R., Weinrich J., Lu X., Jiang S., Chang Q., Navone N.M., Troncoso P., DePinho R.A, & Wang Y.A. (2018). An in vivo screen identifies PYGO2 as a driver for metastatic prostate cancer. Cancer research, 78(14), 3823-3833.
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5

Immunoblotting Protein Expression Analysis

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The procedures for protein sample preparation from cell cultures, protein quantification, immunoblotting, and data analyses were performed as previously described [23 (link)]. The following antibodies were used for immunoblotting analyses: β-actin (#ab6276), DRP1 (#ab56788), and OPA3 (#ab230205) were from Abcam (Cambridge, UK); phospho-DRP1 Ser616 (#3455), Vimentin (#5741), Slug (#9585), N-Cadherin (#13116), E-Cadherin (#3195) and snail (#3879) were from Cell Signaling Technology (Danvers, MA, USA); K-ras (#sc-30) was from Santa-Cruz biotechnology (Santa Cruz, CA, USA); and Tubulin (#BM1452) was from Boster Bio (Pleasonton, CA, USA). Protein bands were detected by chemiluminescence, using an ECL detection kit (Thermo Scientific). When appropriate, bands obtained via Western blot analysis were quantified, using the ImageJ software (http://rsb.info.nih.gov/ij/). Protein expression was normalized by β-actin or Tubulin of the respective samples.
Meng N., Glorieux C., Zhang Y., Liang L., Zeng P., Lu W, & Huang P. (2019). Oncogenic K-ras Induces Mitochondrial OPA3 Expression to Promote Energy Metabolism in Pancreatic Cancer Cells. Cancers, 12(1), 65.
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6

Immunoblot Analysis of Key Signaling Proteins

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The following antibodies were used for immunoblots at a dilution of 1:1000, except where indicated. HRAS (sc-520), NRAS (sc-31; 1:500), KRAS (sc-30; 1:500), FRS2 (sc-8318), CREB (sc-58) and GFP (sc-9996) were from Santa Cruz Biotechnology; pMEK S217/221 (#9121), tMEK (#9122), pERK (#9101), tERK (#4695), FGFR1 (#9740), pFRS2 (#3864), pAKT (#9271), EGFR (#4267), pEGFR (#3777) and pCREB (#9198) were from Cell Signaling Technology; β-Actin (A2228; 1:10000) and Vinculin (V4139) were from Sigma; and NF1 (A300-140A-M) was from Bethyl Laboratories.
Untch B.R., Anjos V.D., Garcia-Rendueles M.E., Knauf J.A., Krishnamoorthy G.P., Saqcena M., Bhanot U.K., Socci N.D., Ho A.L., Ghossein R, & Fagin J.A. (2018). Tipifarnib inhibits HRAS-driven dedifferentiated thyroid cancers. Cancer research, 78(16), 4642-4657.
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