Shandon excelsior es

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

The Shandon Excelsior ES is a tissue processor for the automated dehydration, clearing, and paraffin infiltration of tissue samples. It is designed to efficiently process multiple tissue samples simultaneously.

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Lab products found in correlation

Varistain gemini es automated slide stainer, by Thermo Fisher Scientific (2 mentions) Spot rt3 camera, by Leica camera (2 mentions) Hm 325, by Thermo Fisher Scientific (2 mentions) Microphot fx, by Nikon (2 mentions) Hematoxylin and eosin, by Merck Group (1 mentions) Hm355s, by Thermo Fisher Scientific (1 mentions) Eclipse ti, by Nikon (1 mentions) Varistain gemini es, by Thermo Fisher Scientific (1 mentions) Leica rm2235, by Leica Biosystems (1 mentions) Leica eg 1160, by Leica Biosystems (1 mentions) Leica aperio cs2, by Leica Biosystems (1 mentions) Dp21 digital camera, by Olympus (1 mentions) Bx51 polarized light microscope, by Olympus (1 mentions) Rm2255 microtome, by Leica Biosystems (1 mentions) Easyscan pro 6, by Motic (1 mentions) Spss ver 20, by IBM (1 mentions) Bx 50, by Olympus (1 mentions)

11 protocols using shandon excelsior es

Histomorphometric Analysis of Kidney Tissue

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Immediately after the sacrifice, to avoid any possible post-mortem artefacts, the liver and kidneys were fixed in 10% buffered formalin and stored in 80% ethyl alcohol. They were dehydrated in a graded series of alcohol baths and embedded in paraffin by tissue processor (Shandon Excelsior ES, Thermo Scientific, Waltham, MA, USA). The histological sections of 5 μm thick were prepared using the Microm. HM 325 (Thermo Scientific, Waltham, MA, USA) and stained with hematoxylin/eosin for the examination under a light microscopy (Nikon Microphot FX, Melville, NY, USA) at various magnifications to evaluate the histopathological alterations.
The quantitative histomorphometrical analyses were performed on kidneys. The transverse section was examined by means of an image analysis system (Nis-Elements BR) applied to an optical microscope (Nikon Microphot FX, Melville, NY, USA). The diameter and area were measured for 100 glomeruli/sample using a 10× lens, and the glomerular volume was calculated [24 ].

Tassinari R., Tammaro A., Martinelli A., Valeri M, & Maranghi F. (2023). Sex-Specific Effects of Short-Term Oral Administration of Food-Grade Titanium Dioxide Nanoparticles in the Liver and Kidneys of Adult Rats. Toxics, 11(9), 776.

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Histological Sectioning and Staining of Zebrafish Larvae

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Fin clipped larvae were fixed in 4% PFA at 4 °C overnight and kept in 70% ethanol. At least five embryos or larvae per genotype group were embedded in 1% agarose in 1X TAE buffer. A mould, specifically designed to align zebrafish larvae, was used to produce agarose blocks with identical distributed wells of the same depth. Agarose blocks were gradually dehydrated in an enclosed automated tissue processor (Shandon Excelsior ES, Thermo Scientific) and subsequently embedded in paraffin. The heads of paraffin-embedded larvae were sectioned on a HM 325 manual rotary microtome (Thermo Fisher Scientific) at a thickness of 5 μm. The specimens were stained with hematoxylin and eosin (H&E stain) using Varistain™ Gemini ES Automated Slide Stainer (Thermo Fisher Scientific) according to laboratory protocols. The resulting sections were imaged at ×20 magnification in a SPOT 5.1 software (SPOT Imaging) by a SPOT-RT3 camera mounted on a Leica microscope. Brightness of the images was adjusted for the white background.

Siekierska A., Stamberger H., Deconinck T., Oprescu S.N., Partoens M., Zhang Y., Sourbron J., Adriaenssens E., Mullen P., Wiencek P., Hardies K., Lee J.S., Giong H.K., Distelmaier F., Elpeleg O., Helbig K.L., Hersh J., Isikay S., Jordan E., Karaca E., Kecskes A., Lupski J.R., Kovacs-Nagy R., May P., Narayanan V., Pendziwiat M., Ramsey K., Rangasamy S., Shinde D.N., Spiegel R., Timmerman V., von Spiczak S., Helbig I., Weckhuysen S., Francklyn C., Antonellis A., de Witte P, & De Jonghe P. (2019). Biallelic VARS variants cause developmental encephalopathy with microcephaly that is recapitulated in vars knockout zebrafish. Nature Communications, 10, 708.

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Histological Analysis of Dissected Tumors

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Dissected tumor plugs were kept overnight in freshly prepared formalin solution and dehydrated in tissue processor (Shandon Excelsior ES, Thermo Scientific, Kalamazoo, MI, USA) using reagents provided by DiaPath (DiaPath, Martinengo, Italy). Dehydrated plugs were embedded in paraffin, cut into 4-μm-thick sections on microtome (HM 355S, Thermo Scientific, Kalamazoo, MI, USA), and stained with hematoxylin and eosin (Sigma-Aldrich, St. Louis, MO, USA) in Varistain Gemini automated slide stainer (Thermo Scientific, Kalamazoo, MI, USA), according to a standard protocol. Prepared sections were analyzed under a microscope (Eclipse Ti, Nikon, Tokyo, Japan). Pictures were taken using NIS-Elements BR software. Content of necrotic tissue was assessed using AxioVision 40 V 4.8.2 software (Carl Zeiss Microscopy, Jena, Germany).

Szade K., Zukowska M., Szade A., Collet G., Kloska D., Kieda C., Jozkowicz A, & Dulak J. (2015). Spheroid-plug model as a tool to study tumor development, angiogenesis, and heterogeneity in vivo. Tumour Biology, 37(2), 2481-2496.

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Histological Analysis of Larval Tissues

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VHC- and KA-injected larvae were fixed in 4% paraformaldehyde (PFA) at 4°C overnight and kept in 70% ethanol. At least four larvae per genotype group were embedded in 1% agarose in 1x TAE buffer. A specifically designed mold was used to produce agarose blocks with identically distributed wells of the same depth. Agarose blocks were gradually dehydrated in an enclosed automated tissue processor (Shandon Excelsior ES, Thermo Scientific) and subsequently embedded in paraffin. Heads of paraffin-embedded larvae were sectioned on a HM 325 manual rotary microtome (Thermo Fisher Scientific) at 5 μm thickness. Specimens were stained with haematoxylin and eosin (H&E) using Varistain™ Gemini ES Automated Slide Stainer (Thermo Fisher Scientific). Resulting sections were imaged at 20x and 40x magnification in SPOT 5.1 software (SPOT Imaging) by a SPOT-RT3 camera mounted on a Leica microscope. Brightness of the images was adjusted for the white background.

Heylen L., Pham D.H., De Meulemeester A.S., Samarut É., Skiba A., Copmans D., Kazwiny Y., Vanden Berghe P., de Witte P.A, & Siekierska A. (2021). Pericardial Injection of Kainic Acid Induces a Chronic Epileptic State in Larval Zebrafish. Frontiers in Molecular Neuroscience, 14, 753936.

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Tissue Histology Protocol for Ethanol-Fixed Samples

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Ethanol fixed samples were processed in a Shandon Excelsior ES tissue processor (Thermo Scientific, Waltham, MA) with alcohol dehydration and xylene infiltration. Standard hematoxylin-eosin staining was performed to assess for necrosis and inflammatory infiltrate.

Ding N., Chen G., Hoffman R., Loughran P.A., Sodhi C.P., Hackam D.J., Billiar T.R, & Neal M.D. (2014). TLR4 Regulates Platelet Function and Contributes to Coagulation Abnormality and Organ Injury in Hemorrhagic Shock and Resuscitation. Circulation. Cardiovascular genetics, 7(5), 615-624.

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Histological Analysis of Skin Biopsies

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Skin biopsies were dehydrated and cleared using an automated Tissue Processor (Shandon Excelsior ES, Thermofisher Scientific, Waltham, MA, USA). The biopsies were then embedded in paraffin using a tissue embedding machine (Leica EG1160, Leica Biosystem, Wetzlar, Germany). A 4 μm thick section was taken from each biopsy using a manual microtome (Leica RM2235, Leica Biosystem, Wetzlar, Germany) and stained with hematoxylin and eosin. Staining was performed on an auto-stainer (Leica ST5020, Leica Biosystems, Wetzlar, Germany) according to the manufacturer's instructions. Each section was scanned (Leica Aperio CS2, Leica Biosystems, Wetzlar, Germany) and examined on a computer screen.

Naseem M.N., Allavena R., Raza A., Constantinoiu C., McGowan M., Turni C., Kamran M., Tabor A.E, & James P. (2023). Pathology and pathogenesis of cutaneous lesions in beef cattle associated with buffalo fly infestation. Frontiers in Veterinary Science, 9, 971813.

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Decalcification and Sectioning of Tissue Constructs

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The staples were gently removed from the tissue and the samples were decalcified using 5M EDTA at room temperature for 3 weeks prior to dehydration and paraffin embedding using a tissue processor (Shandon™ Excelsior ES, Thermo Scientific, Waltham, USA). 5μm thick longitudinal slices of the constructs were sectioned and were stained with H&E.

Lui H., Vaquette C., Denbeigh J.M., Bindra R., Kakar S, & van Wijnen A.J. (2020). Multiphasic scaffold for scapholunate interosseous ligament reconstruction: a study in the rabbit knee. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(8), 1811-1824.

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Histological Analysis of Lymphatic Vessels

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The SiLN and PALN were removed on day 0^T (without drug injection) or 9^T, immersed in 10% formalin solution for 4 days at room temperature, and dehydrated in 100% ethanol for 2 days at room temperature. The ethanol‐exchanged organs were paraffin‐embedded in a closed automated inclusion system (Shandon Excelsior ES™; Thermo Fisher Scientific), and 2.5‐µm sections were prepared. In addition to hematoxylin‐eosin (HE) staining, lymphatic endothelial cells were immunohistochemically stained with rabbit anti‐LYVE‐1 antibody (1:250; 103‐PA50AG; ReliaTech). Images were acquired with a BX51 polarized light microscope equipped with a DP21 digital camera (Olympus).

Fukumura R., Sukhbaatar A., Mishra R., Sakamoto M., Mori S, & Kodama T. (2021). Study of the physicochemical properties of drugs suitable for administration using a lymphatic drug delivery system. Cancer Science, 112(5), 1735-1745.

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Histological Analysis of AFL and BCC

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For histology of AFL channels, we treated healthy skin with CO₂-laser and isolated skin biopsies shortly after. For histology of microscopic BCCs, we isolated samples from non-treated tumor-induced skin. All skin biopsies were put into paraformaldehyde-soaked nylon filters (Leica Biosystems, product number 3801085, Maarn, the Netherlands) to reduce tissue stretching, placed in tissue cassettes, and submerged in stabilized and buffered 4% formaldehyde (VWR Chemicals, catalog number 9713.1000, Leuven, Belgium). Samples were then embedded in paraffin in a Shandon Excelsior ES (Thermo Fisher Scientific, Cheshire, UK) before being cut into 3-µm-thick sections with a RM2255 microtome (RRID:SCR_020229, Leica Biosystems, Nussloch, Germany). Tissue sections were stained with hematoxylin (Merck, mixed by Apoteket RegionH, Copenhagen, Denmark) and eosin (Acros, mixed by Apoteket, Copenhagen, Denmark), and microscopy and digitalization were performed on a Motic EasyScan Pro 6 (Motic, Barcelona, Spain) using a 20× objective.

Pedersen K.K., Granborg J.R., Lerche C.M., Litman T., Olesen U.H, & Hædersdal M. (2024). Ablative fractional laser treatment reduces hedgehog pathway gene expression in murine basal cell carcinomas. Lasers in Medical Science, 39(1), 55.

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Laryngeal Fistula Evaluation in Rats

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Rats were sacrificed after 14 days, and the larynx was extracted without damaging the pharynx, upper oesophagus or skin integrity. Specimens were fixed in formaldehyde for 24 hours and sectioned. Fistula formation between the pharynx and oesophagus and the skin was evaluated macroscopically and scored. Cutaneous, subcutaneous and oesophageal extension of the fistula was evaluated macroscopically and a midline section was taken, blocked and processed for 13 hours in an automatic tissue processor (Shandon Ex-celsior ES, Thermo Scientific, Runcorn, England). Following processing, 2-µm sections were taken from paraffinised tissue and stained with haematoxylin and eosin (H&E). Stained sections were observed with a light microscope (Olympus Bx-50, Olympus Optical, Tokyo, Japon) by a pathologist blinded to the evaluation. Macroscopic PCF closure, fibroblast concentration, collagen formation, and surface epithelisation were evaluated and scored.
Fibroblast concentration was evaluated as follows: fibroblast cell count <5, minimal; 5-15, moderate; 15-50, high; >50, very high. Squamous epithelial cells were identified and epithelised and ulcerated areas were evident. For descriptive statistics, rates and frequencies were used. For variance analysis, IBM SPSS ver. 20.0 (IBM Co., Armonk, NY, USA) was used with a chi-square test, Fisher exact test, as appropriate.

Kucuk N., Sari M., Midi A., Yumusakhuylu A.C., Findik O, & Binnetoglu A. (2015). Effectiveness of Recombinant Human Growth Hormone for Pharyngocutaneous Fistula Closure. Clinical and Experimental Otorhinolaryngology, 8(4), 390-395.

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