Mcf 7

Human gastric cancer cell lines (AGS, SNU638 and SNU719) and breast cancer cell lines (BT549, MCF7 and MDA-MB231), mouse melanoma cell line B16F10 were purchased from the Korean Cell Line Bank (KCLB). Human cancer cell lines were cultured in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin (Capricorn scientific) at 37 °C in a humidified atmosphere of 5% CO₂, and B16F10 was cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium under the same conditions. TQ was suspended in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA).

The human breast cancer cell lines MCF-7 and MDA-MB-231 were obtained from the Korea Cell Line Bank (KCLB, Seoul, Korea). Both cell lines were grown in Dulbecco’s Modified Essential Medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (HyClone), and were maintained at 37 °C in a humidified incubator containing 5% CO₂. We used breast cancer cell lines up to 15 passages after purchasing cell lines from KCLB and the cell lines were authenticated by the KCLB and showed >90% similarity in a short tandem repeats (STR) DNA fingerprint profile when compared with the American Type Culture Collection (ATCC) and Korean Cell Line Bank (KCLB) databases. Cells were plated at a density of 1 × 10⁶ cells in 10-cm culture dishes. To establish primary mammospheres, single-cell suspensions of MCF-7 and MDA-MB-231 cells were seeded at a density of (3.5∼4) × 10⁴ and (0.5∼1) × 10⁴ cells/well, respectively, in ultralow attachment six-well plates containing 2 mL of complete MammoCult^TM medium (StemCell Technologies; Vancouver, BC, Canada), which was supplemented with 4 μg/mL of heparin, 0.48 μg/mL of hydrocortisone, 100 U/mL of penicillin, and 100 μg/mL of streptomycin. The cells were incubated for seven days in a 5% CO₂ incubator at 37 °C.

The human breast cancer cell line MCF-7 was obtained from the Korean Cell Line Bank (Seoul, South Korea) and maintained in RPMI 1640 medium (Gibco BRL, Grand Island, NY, USA), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The normal human breast cell line MCF10A was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in Dulbecco’s modified Eagle’s medium (Welgene, South Korea) supplemented with 10% FBS and 1% penicillin/streptomycin. Both cell lines were maintained at 37 °C in 5% CO₂.