LNCaP-P53KO (7 (link)) and LASCPC-01 (10 (link)) were described previously. MR42D (gift from A. Zoubeidi, University of British Columbia, Vancouver, British Columbia, Canada), LNCaP–N-Myc (gift from D. Rickman, Weill Cornell Medicine, New York, New York, USA), and DKO and TKO cells (gifts from L. Ellis, Cedars-Sinai Medical Center, Los Angeles, California, USA) were cultured as described previously (6 (link), 7 (link), 9 (link)–11 (link), 56 (link)). LNCaP (CRL-1740) and NCI-H660 cells (CRL-5813) were purchased from ATCC and cultured according to their recommendation. All cell lines were validated with STR DNA fingerprinting by Genetica Cell Line Testing (a LabCorp brand) and regularly tested for Mycoplasma contamination by the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-318).
Nci h660 cells
Manufactured by American Type Culture Collection
Sourced in United States
NCI-H660 cells are a cell line derived from a human prostate cancer tumor. They are commonly used in cancer research to study the biology and treatment of prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Genetica cell line testing, by Lonza (1 mentions)
Ultra low attachment 96 well plate, by Corning (1 mentions)
Rock inhibitor y 27632, by STEMCELL (1 mentions)
Matrigel, by BD (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Corning (1 mentions)
Celltiter glo 3d viability assay, by Promega (1 mentions)
Dimethyl sulfoxide (dmso), by Selleck Chemicals (1 mentions)
Hepatocyte growth media, by Corning (1 mentions)
Gentamicin amphotericin, by Lonza (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rwpe 1 cells, by American Type Culture Collection (1 mentions)
Pwr 1e, by American Type Culture Collection (1 mentions)
Lncap cells, by Cytion (1 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
β estradiol, by Merck Group (1 mentions)
4 protocols using nci h660 cells
1
Prostate Cancer Cell Line Characterization
2
EPHB4 Inhibitor Treatment of Organoid Cultures
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NCI-H660 cells (ATCC) were seeded at 5000 cells/well density in ultra-low attachment 96-well plates (Corning) and cultured in Hepatocyte growth media (Corning) supplemented with 10 ng/ml epidermal growth factor (Corning), 5% heat inactivated charcoal stripped FBS, 1X Glutamax (Gibco), 5% Matrigel (BD Biosciences), 10 µM ROCK inhibitor (Y-27632, STEMCELL Technologies), and 1X Gentamicin/Amphotericin (Lonza), as described previously55 (link). At day 8, organoids were treated with NVP-BHG 712 (EPHB4i) from Selleck Chemicals or DMSO (Veh.), and viability was assessed at day 15 as per manufacturer’s protocol (Cell Titer Glo-3D viability assay, Promega).
3
Culturing human prostate cell lines
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Human prostate epithelial cell lines [PWR-1E (CRL-11611) and RWPE-1 cells (CRL-11609)] and PCa cell lines [PC-3 (CRL-1435™), DU145 (HTB-81), C4-2 (CRL-3314), 22Rv1 (CRL-2505) and NCI-H660 cells (CRL-5813)] were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and LNCaP cells were purchased from Cell Lines Service (CLS, Eppelheim, Germany). The cells were cultured at 37°C in 5% CO2 in RPMI-1640 medium (GENOM, Hangzhou, China) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco/Thermo Fisher Scientific). The medium was changed every 8-10 h.
4
Cell Culture Protocols for Cancer Research
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LNCaP-NMYC cells were a generous gift from David Rickman at Weill Cornell Medicine (New York, NY, USA), and were cultured in RPMI supplemented with 10% fetal bovine serum (FBS). HO15.19 cells cultured in DMEM supplemented with 10% FBS were a generous gift from John Sidivy at Brown University (Providence, RI, USA). NCI-H660 cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI supplemented 5% FBS, 1% Insulin-Transferrin-Selenium (Thermo Fisher, 41400-045) (Waltham, MA, USA), 10nM b-estradiol (Sigma, E8875) (St. Louis, MO, USA), 10 nM hydrocortisone (Sigma H0888) and 1% matrigel. IMR32 cells were purchased from ATCC and were cultured in Eagle’s Minimum Essential Medium (EMEM) supplemented with 10% FBS.
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