All chemical reagents used in this work were of analytical grade (AR) and used without further purification. Polyacrylonitrile (PAN, Mw = 90,000) were purchased from J&K Scientific, Ltd. (Beijing, China) 3,4-ethylenedioxythiophene, sodium borohydride, and polystyrene sulfonate (PSS, Mw = 80,000) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium hydroxide, ammonium chloride, nickel sulfate hexahydrate, sodium citrate, sodium hypophosphite, ferric chloride, ethanol, N,N-dimethylformamide, and trichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Trichloromethane
Manufactured by Sinopharm
Sourced in China, Japan
Trichloromethane is a colorless, dense liquid chemical compound. It has the molecular formula CHCl3. Trichloromethane is commonly used as a solvent in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sinopharm (7 mentions)
Isopropyl alcohol, by Sinopharm (6 mentions)
Sodium hydroxide (naoh), by Sinopharm (4 mentions)
Sodium chloride (nacl), by Sinopharm (4 mentions)
Diethyl ether, by Sinopharm (3 mentions)
Ethyl acetate, by Sinopharm (3 mentions)
Trizol, by Takara Bio (3 mentions)
Methanol, by CNW Technologies (3 mentions)
Pyridine, by CNW Technologies (3 mentions)
N hexane, by CNW Technologies (3 mentions)
Acetone, by Sinopharm (3 mentions)
Absolute ethanol, by Sinopharm (3 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (3 mentions)
Methanol, by Sinopharm (3 mentions)
Ammonium chloride, by Sinopharm (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Acetic acid, by Sinopharm (2 mentions)
Acetic anhydride, by Sinopharm (2 mentions)
Glacial acetic acid, by Sinopharm (2 mentions)
Triethylamine, by Sinopharm (2 mentions)
38 protocols using trichloromethane
1
Synthesis of Conductive Polymer Composites
2
Synthesis and Evaluation of PEG-Modified Nanocarrier
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PEG2k-NH2 was purchased from Xiamen SINOPEG Biotechnology Co., Ltd. (Xiamen, China). Methylene chloride, dimethyl sulfoxide (DMSO), N, N-dimethylformamide (DMF), dichloromethane (DCM), trichloromethane (TCM), diethyl ether, and hydrogen peroxide were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Succinic anhydride 1-ethyl-3(3-dimethylpropylamine) (EDCI) was offered by Beijing Bailingwei Technology Co., Ltd. (Beijing, China). Hexadecyl isocyanate and MTT were obtained from Sigma-Aldrich (Shanghai) Trading Co., Ltd. (Shanghai, China). Carboplatin was obtained from Shandong Boyuan Pharmaceutical Co., Ltd. (Jinan, China). St. Nile red and phosphotungstic acid were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). RPMI1640 culture medium and newborn fetal bovine serum were obtained from GIBCO Biotechnology Co., Ltd. (Carlsbad, CA). H460, A549 tumor cell lines were obtained from the Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China). SPF balb/c/nu/nu nude mice were provided by Viton Lihua.
3
Quantitative Analysis of DQ Standard
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DQ Standard (CAS: 18507-89-6, Purity ≥ 96.8%) was purchased from Dr. Ehrenstorfer DmbH. (Augsburg, Germany). Pyridine (CAS: 110-86-1 analytical grade) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Methanol and acetonitrile were HPLC grade and purchased from Merck Inc. (Fairfield, OH, USA). Analytically pure n-hexane, ethyl acetate, acetic anhydride (CAS: 108-24-7), acetic acid (CAS: 64-19-7) and trichloromethane (CAS: 67-66-3) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ultrapure water had a resistivity of 18.25 MΩ * cm (25 C) and used to be grade I water in accordance with the National Standard for Laboratory Water (GB6682-1992).
Organic section nylon needle filters (13 mm × 0.22 μm) were obtained from Shanghai Ampu Experimental Technology Co., Ltd. (Shanghai, China). A chromatographic column (TG-5MS 30.0 m × 0.25 μm × 0.25 mm) was acquired from Thermo Fisher (Waltham, MA, USA). The chromatographic column StrataTM-X (60 mg/3 mL, tubes) was acquired from Phenomenex. (Torrance, CA, USA); the extraction column Cleanert PEP (60 mg/3 mL, 50/pkg) was procured from Agilent Technologies. (Beijing, China). The Waters Oasis HLB extraction column (60 mg, 3 mL) was procured from Waters. (Milford, MA, USA).
Organic section nylon needle filters (13 mm × 0.22 μm) were obtained from Shanghai Ampu Experimental Technology Co., Ltd. (Shanghai, China). A chromatographic column (TG-5MS 30.0 m × 0.25 μm × 0.25 mm) was acquired from Thermo Fisher (Waltham, MA, USA). The chromatographic column StrataTM-X (60 mg/3 mL, tubes) was acquired from Phenomenex. (Torrance, CA, USA); the extraction column Cleanert PEP (60 mg/3 mL, 50/pkg) was procured from Agilent Technologies. (Beijing, China). The Waters Oasis HLB extraction column (60 mg, 3 mL) was procured from Waters. (Milford, MA, USA).
4
Extraction and Quantification of RNA from BV2 Microglia
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BV2 microglia were collected after OGD/R. Total RNA was isolated by treating the cells with 500 mL of RNAiso or TRIzol (Takara, Tokyo, Japan) and 100 mL of trichloromethane (Sinopharm) for 5 minutes and then centrifuging the solution for 15 minutes. The supernatant was diluted in an equal volume of isopropanol, mixed for 10 minutes, and centrifuged for 10 minutes. Afterward, 1 mL of 75% ethanol (Sinopharm) was added to the mixture, which was then centrifuged for 5 minutes. The pellet was then resuspended in RNase-free H2O (Takara), and the mixture was centrifuged at 12,000 × g, 4°C. Next, 1000 ng of purified total RNA was subjected to reverse transcription at 37°C for 15 minutes, then 5 seconds at 85°C with PrimeScript RT Master Mix (Takara). SYBR Premix Ex Taq (Takara) was used for quantitative polymerase chain reaction (qPCR) in a 20 μL reaction, using the reverse-transcribed cDNA as a template. The primer sequences are shown in Additional Table 1
5
Synthesis and Evaluation of Novel Triazole Derivatives
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An AdvanceIII HD-400 NMR spectrometer (Bruker, Fällanden, Switzerland), Tensor27 IR spectrometer (Bruker), Vario ELIII elemental analyzer (Elementar Analysensysteme GmbH, Langenselbold, Germany), and SGW X-4 micro-melting-point apparatus (Shanghai Shen Guang Instrument Co. Ltd., Shanghai, China) were used in all procedures. Para-hydroxybenzaldehyde (analytical purity), cyanuric chloride (analytical purity), 1,4-dioxane (analytical purity), triethylamine, 10% anhydrous Na2CO3 (analytical purity), ethyl acetate (analytical purity), glacial acetic acid (analytical purity), 4-amino-1,2,4-triazole, trichloromethane (analytical purity), ethanol (analytical purity), sodium carboxymethyl cellulose, and silica gel GF254 (analytical purity) were purchased from SinoPharm (Shanghai, China). The human lung adenocarcinoma cell line A549 and human hepatoma cell line Bel7402 were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 culture medium, trypsinase, and fetal calf serum were obtained from Gibco (Carlsbad, CA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Invitrogen (Carlsbad, CA, USA).
6
Synthesis of Biodegradable Polymer Blend
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CDA with a DS of 2.46 was obtained from Nantong Acetate Co. L-LA was purchased from Shandong Longjing New Material Technology Co. Stannous octanoate [Sn(Oct)2] (98.5% purity) was purchased from Aladdin Biochemical Technology Co. (Shanghai, China). Trichloromethane was purchased from Sinopharm Chemical Reagent Co., Ltd., and anhydrous methanol was purchased from Shanghai Lingfeng Chemical Reagent Co. The toluene was provided by Sinopharm Chemical Reagent Co. (Shanghai, China).
7
Metabolomic Analysis of Larimichthys Crocea Roe
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The roe of Larimichthys Crocea was obtained from Fujian Yuehai Aquatic Food Co., Ltd. (Ningde, China). All chemicals and solvents were either of analytical or HPLC grade. The water, methanol, pyridine, n-hexane, methoxylamine hydrochloride (97%), and BSTFA with 1% TMCS were bought from CNW Technologies GmbH (Düsseldorf, Germany). The trichloromethane was sourced from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The L-2-chlorophenylalanine came from Shanghai Hengchuang Bio-technology Co., Ltd. (Shanghai, China).
8
Analysis of Organic Compounds by GC-MS
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All chemicals and solvents were analytical or HPLC grade. Water, methanol, pyridine, n-hexane, methoxylamine hydrochloride (97%), and bis (trimethylsilyl) trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) were purchased from CNW Technologies GmbH (Düsseldorf, Germany). Trichloromethane was obtained from Sinopharm Chemical Reagent Co.; Ltd. (Shanghai, China). L-2-chlorophenylalanine was obtained from Shanghai Hengchuang Biotechnology Co.; Ltd. (Shanghai, China).
9
Synthesis and Characterization of Lipid-based Nanoparticles
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OEA (purity grade > 90.0%), SPC (purity grade >90%), and cholesterin (purity grade >90%) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). DSPE-PEG (MWCO 2000) was obtained from Ponsure Biotechnology Co., Ltd. (Shanghai, China). Trichloromethane was purchased from Sinopharm Chemical Reagent Co., Ltd. All chemical reagents were of analytical grade and used without further purification unless otherwise stated.
10
Analytical Reagents for Metabolomic Studies
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All chemicals and solvents were analytical or HPLC grade. Trichloromethane was from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Water, methanol, pyridine, n-hexane, methoxylamine hydrochloride (97%), N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (BSTFA with 1% TMCS) were purchased from CNW Technologies GmbH (Düsseldorf, Germany). L-2-chlorophenylalanine was from Shanghai Hengchuang Bio-Technology Co., Ltd. (Shanghai, China).
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