The normal human breast cell line Hs 578Bst, and the breast cancer cell lines MCF-7 and MDA-MB-231 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). Hs 578Bst cells were cultured with ATCC Hybri-Care medium (cat no. 46-X) containing 1.5 g/l sodium bicarbonate, 30 ng/ml mouse epidermal growth factor (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and 10% fetal bovine serum (Sigma-Aldrich; Merck KGaA). MCF-7 cells were cultured with ATCC-formulated Eagle's minimum essential medium (cat no. 30–2003) containing 0.01 mg/ml human recombinant insulin (Sigma-Aldrich; Merck KGaA) and 10% fetal bovine serum (Sigma-Aldrich; Merck KGaA). MDA-MB-231 cells were cultured with ATCC-formulated Leibovitz's L-15 medium (cat no. 30-2008) containing 10% fetal bovine serum (Sigma-Aldrich; Merck KGaA). Cells were cultured at 37°C, and harvested during logarithmic growth phase for subsequent experiments.
Hybri care medium
Manufactured by Merck Group
Sourced in Germany
Hybri-Care medium is a sterile, liquid culture medium designed for the maintenance and growth of hybridoma cell lines. It provides the necessary nutrients and growth factors to support the proliferation of these cells in vitro. The composition of the medium is optimized to support the specific requirements of hybridoma cell cultures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Mouse epidermal growth factor, by Merck Group (1 mentions)
Recombinant human insulin, by Merck Group (1 mentions)
Mcf 7, by Merck Group (1 mentions)
Mda mb 231, by Merck Group (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Hs 578bst, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Merck Group (1 mentions)
Leibovitz s l 15 medium, by Merck Group (1 mentions)
Mccoy s 5a medium, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
2 protocols using hybri care medium
1
Breast Cell Lines: Culture and Characterization
2
Culturing Human Cancer Cell Lines
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All cells were purchased from the Taiwan Bioresource Collection and Research Center (Hsinchu, Taiwan). The MDA-MB-231, HCC1937, T-47D and ZR-75-1 were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum (Gibco, Grand Island, NY). The BT474 was cultured in 90% Hybri-Care Medium (Sigma-Aldrich, St. Louis, MO) with 30 ng/ml EGF and 10% fetal bovine serum. The MDA-MB-453 was cultured in 90% Leibovitz's L-15 medium (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum and without CO2. The MCF-7 and Hs 578T were cultured in Dulbecco's Modified Eagle Medium (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum. The MDA-MB-361 was cultured in 80% Leibovitz's L-15 medium with 20% fetal bovine serum and without CO2. The U-2 OS was cultured in McCoy’s 5A medium (Sigma-Aldrich, St. Louis, MO) with 10% fetal bovine serum.
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