Wnt1 cre2

Manufactured by Jackson ImmunoResearch

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The Wnt1-Cre2 is a genetic recombination tool used in biomedical research. It enables targeted modification of gene expression in specific cell populations during embryonic development.

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9 protocols using wnt1 cre2

Generating Genetically Modified Mouse Models

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Dhcr7^−/− mice^{85 (link)} were a gift from Dr. Forbes D. Porter (The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA). Insig1^F/F;Insig2^−/− (The Jackson Laboratory, #005939)^{12 (link)} and Wnt1-Cre2 (The Jackson Laboratory, #022501)^{22 (link)} mice were obtained from The Jackson Laboratory and crossed to generate Insig1/2 cKO mice. Gli1-LacZ mice (The Jackson Laboratory, #008211)^{39 (link)} were obtained from The Jackson Laboratory and crossed with Dhcr7^+/− and Wnt1-Cre2;Insig1^F/+;Insig2^−/− mice in order to generate Dhcr7^−/−;Gli1-LacZ, Dhcr7^+/+;Gli1-LacZ, Insig1/2 cKO;Gli1-LacZ, and Insig1^F/F;Insig2^−/−;Gli1-LacZ mice. Topgal (The Jackson Laboratory, #004623)^{86 (link)} and Axin2^LacZ/+ (The Jackson Laboratory, #009120)^{87 (link)} mice were obtained from The Jackson Laboratory and crossed with Dhcr7^−/− mouse line to generate Dhcr7^−/−;Topgal and Dhcr7^+/+;Topgal, Dhcr7^−/−;Axin2^LacZ/+, and Dhcr7^+/+;Axin2^LacZ/+ mice. Genotyping was performed using PCR primers, as previously described.^{12 (link),22 (link),85 (link)} Pregnant females were treated with simvastatin (S6196; Sigma-Aldrich) at a dose of 10 mg/kg⁻¹ body weight (BW) from E12.5 to E18.5, or from day 7 to day 42, administered by intraperitoneal injection.

Suzuki A., Ogata K., Yoshioka H., Shim J., Wassif C.A., Porter F.D, & Iwata J. (2020). Disruption of Dhcr7 and Insig1/2 in cholesterol metabolism causes defects in bone formation and homeostasis through primary cilium formation. Bone Research, 8, 1.

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Genetic Mouse Strains for Research

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All procedures and experiments were performed according to the guidelines of the Canadian Council on Animal Care and approved by the animal Care Committee of the Montreal Children's Hospital. WT CD1, mT/mG Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/J, (Soriano, 1999 (link)), Wnt1-Cre2 (Lewis et al., 2013 (link)) and β-actin-Cre (Lewandoski et al., 1997 (link)) mice on the C57BL/6J genetic background were purchased from The Jackson Laboratory. The R26R strain [Gt (ROSA)26Sor^tm1Sor (Muzumdar et al., 2007 (link))] on the mix C57BL/6J;129/S4 genetic background was a kind gift from Dr Nagano (Department of Obstetrics and Gynecology, McGill University, Montreal, Canada). All strains were maintained on the CD1 genetic background. The Trp53^tm1brn mouse line with loxP sites flanking exons 2-10 of the Trp53 gene was purchased from The Jackson Laboratory (Trp53^loxP/+) (stock #008462) (Marino et al., 2000 (link)).

Alam S.S., Kumar S., Beauchamp M.C., Bareke E., Boucher A., Nzirorera N., Dong Y., Padilla R., Zhang S.J., Majewski J, & Jerome-Majewska L.A. (2022). Snrpb is required in murine neural crest cells for proper splicing and craniofacial morphogenesis. Disease Models & Mechanisms, 15(6), dmm049544.

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Spatiotemporal Cardiac Lineage Tracing

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All animals were maintained in an American Association for Accreditation of Laboratory Animal Care (AAALAC)–approved animal facility at the University of Miami, Miller School of Medicine, and procedures were performed using Institutional Animal Care and Use Committee–approved protocols according to National Institutes of Health (NIH) standards. The Isl1-nLacZ mice have been described before (14 (link)). The IRG (stock no. 008705), tdTomato (stock no. 007914), Confetti (stock no. 017492), Wnt1-Cre2 (stock no. 022501), Isl1-MerCreMer (stock no. 029566), Isl1^fl/fl (stock no. 028501), Dicer^fl/fl (stock no. 006366), Isl1-Cre (stock no. 024242), and Wnt1/GAL4/Cre-11 (stock no. 003829) mice were obtained from the Jackson laboratory. The Mef2c-AHF-Cre mice were cryorecovered at the University of Miami, Sylvester Comprehensive Cancer Center’s Transgenic animal facility, from material obtained from the Mutant Mouse Regional Resource Centers (MMRRC, strain ID: 30262). The Wnt1-Cre2 mice were bred through the female germ line. The Mef2c-AHF-Cre mice were bred through the male germ line. Genotyping was performed by an independent provider via an automated real-time PCR system (Transnetyx). All analyses were performed in age-matched males and female littermates from multiple litters.

Hatzistergos K.E., Durante M.A., Valasaki K., Wanschel A.C., Harbour J.W, & Hare J.M. (2020). A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract. Science Advances, 6(49), eaba9950.

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Conditional Ift46 Knockout Mice for Fate Mapping

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Conditional Ift46^F/F mice¹⁹ (link) and Wnt1-Cre2 mice were used in the experiments. Wnt1-Cre2;Ift46^F/F (NC-Ift46^F/F) mice were generated by crossing Ift46^F/F mice with Wnt1-Cre2;Ift46^F/+ mice. Wnt1-Cre2, KRT14-Cre, Gli1^lacZ, CBF:H2B-Venus, and ROSA^mTmG mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). For cell fate mapping of NCCs, Wnt1-Cre2;Ift46^F/+ mice were crossed with Ift46^F/F carrying the cell membrane-targeted ROSA^mTmG reporter. Gli1^lacZand CBF:H2B-Venus reporters were used to assess Shh and Notch signal activities, respectively. For corneal epithelial fate mapping, KRT14-Cre;Ift46^F/+;ROSA26 and KRT14-Cre;Ift46^F/F;ROSA26 mice were generated by crossing KRT14-Cre;Ift46^F/+ mice with Ift46^F/F;ROSA26 mice. Genomic DNA from yolk sacs or tails by proteinase K treatment underwent PCR for DNA genotyping. Embryonic age was determined, with noon on the day of the vaginal plug designated as embryonic day 0.5 (E0.5). All experimental procedures complied with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and the experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee of Ewha Womans University.

Seo S., Sonn S.K., Kweon H.Y., Jin J., Kume T., Ko J.Y., Park J.H, & Oh G.T. (2024). Primary Cilium in Neural Crest Cells Crucial for Anterior Segment Development and Corneal Avascularity. Investigative Ophthalmology & Visual Science, 65(3), 30.

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Transgenic Mouse Lines for Brain Imaging

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For PEGASOS-cleared mouse: Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691) were purchased from GemPharmatech (Nanjing, China). R26-mScarlett^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20 °C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.
For CUBIC-L/R cleared mouse: A fixed brain of 9-week-old female Thy1-YFP-H Tg mice (B6.Cg-Tg(Thy1-YFP)HJrs/J, The Jackson Laboratory, Identifier: 003782)^{63 (link)} was provided from National Institute for Physiological Sciences (Okazaki, Japan) under the material transfer agreement with Juntendo University. All animal experiments were approved by Juntendo University (1569-2022279 and 1372-2022211), and National Institute for Physiological Sciences (22A044).

Prince M.N., Garcia B., Henn C., Yi Y., Susaki E.A., Watakabe Y., Nemoto T., Lidke K.A., Zhao H., Remiro I.S., Liu S, & Chakraborty T. (2024). Signal improved ultra-fast light-sheet microscope for large tissue imaging. Communications Engineering, 3, 59.

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Transgenic Mouse Models for Neural Lineage Tracing

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Following mice were purchased from the Jackson Lab with genotypes including Thy1-EGFP-M (JAX# 007788), Ai14 (JAX# 007908), tTA^flox (JAX# 008600), tetO-H2BGFP (JAX# 005104) and Wnt1-Cre2 (JAX# 022501). Tg(Cdh5-CreERT2) mice (Strain NO.T014691）were purchased from GemPharmatech (Nanjing, China). R26-mScarlett ^flox reporter was generated by Hu Zhao lab in the Chinese Institute for Brain Research. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Chinese Institute for Brain Research. For tamoxifen treatment, tamoxifen (Sigma-Aldrich, T5648) was dissolved in corn oil (Sigma-Aldrich, C8267) at 20 mg/ml. The solution was kept at −20°C and delivered via intraperitoneal injection or oral gavage for postnatal treatments.

Prince M.N., Garcia B., Henn C., Yi Y., Susaki E.A., Watakabe Y., Nemoto T., Lidke K.A., Zhao H., Remiro I.S., Liu S, & Chakraborty T. (2023). Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging. Research Square.

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Genetic Mouse Models for Epigenetic Regulation

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Wnt1-Cre2 (Jackson Laboratory, 022137)(Lewis et al., 2013 (link)), Mesp1-Cre (Saga et al., 1999 (link)), R26R-LacZ (Soriano, 1999 (link)), and Hdac2^Flox (Anokye-Danso et al., 2011 (link)) mice have been previously described. Frozen embryos of Hdac1^{tm1a(EUCOMM)Wtsi} (strain EM:04097) were obtained from the European Mouse Mutant Archive (EMMA). The University of Massachusetts Medical School Transgenic Animal Facility regenerated cryopreserved embryos. The knockout-first allele, Hdac1^{tm1a(EUCOMM)Wtsi}, mice were bred with wild type mice and are annotated Hdac1^LacZ/+. Hdac1^{tm1a(EUCOMM)Wtsi} (Hdac1^LacZ) mice were bed with ACTB-FLPe (Jackson Laboratory, 003800) to generate Hdac1^Flox mice. Hdac1^Flox mice were bred with Hdac2^Flox, R26R-LacZ, and Wnt1-Cre2 mice to generate mice for analysis. All animal protocols were approved by the University of Massachusetts Medical School Institutional Animal Care and Use Committee (IACUC).

Milstone Z.J., Lawson G, & Trivedi C.M. (2017). Histone deacetylase 1 and 2 are essential for murine neural crest proliferation, pharyngeal arch development and craniofacial morphogenesis. Developmental dynamics : an official publication of the American Association of Anatomists, 246(12), 1015-1026.

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Genetic mouse models for neuroscience

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Age and sex-matched Gfra2^−/−REF12, Nes-gfp^{61 (link)} (generously provided by G.E. Enikolopov), FVB/N-Adrb3tm1Lowl/J (Stock number 006402), B6;129 × 1-Nrtn^tm1Jmi/J (Stock number 012238), B6.129S7-Chrna7^tm1Bay/J (Stock number 003232), ChATBAC-eGFP (Stock number 007902), α7nAChRflox (Stock number 026965), B6.129(Cg)-Lepr^{tm2(cre)Rck/J} (Stock number 008320), Nes-cre^ERT2(REF ^{62 (link)}⁾ (generously provided by G. Fishell), B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J (Stock number 008610) (Jackson Laboratories), and congenic CD45.1 and CD45.2 C57BL/6 J mice (Charles River) were used in this study. For genetic lineage tracing, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (Ai14D) reporter mice (Stock number 007908) were crossed with B6.129S-Chat^tm1(cre)Lowl/MwarJ mice (Chat-IRES-Cre) (Stock number 031661) and Wnt1-Cre2 (Stock number 022501) (Jackson Laboratories). Mice were housed in specific pathogen-free facilities. All experiments using mice followed protocols approved by the Animal Welfare Ethical Committees at the University of Cambridge (PPL 70/8406 and PPL P0242B783). All experiments were compliant with EU recommendations.

Fielding C., García-García A., Korn C., Gadomski S., Fang Z., Reguera J.L., Pérez-Simón J.A., Göttgens B, & Méndez-Ferrer S. (2022). Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis. Nature Communications, 13, 543.

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Spatiotemporal Transcriptomic Mapping of Mouse Tissues

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All mouse work was performed in accordance with the Institutional Animal Care and Use Committees (IACUC) and relevant guidelines of Boston Children’s Hospital and Masaryk University.
Embryonic day (E) 16.5 embryos were obtained from time-pregnant CD-1 dams for embryonic scRNA-seq analyses. For snRNA-seq analyses, E16.5 embryos, adult (4 months) and aged (20 months) C57BL/6J males and females were used. All animals were housed under 12hr/12hr day night cycle with access to standard chow and water ad libitum.
CD1(ICR)– Charles River Laboratories Strain 022.
Cx3cr1-GFP (catalog #: 005582, The Jackson Laboratory)
C57BL/6 – Charles River Laboratories
C57BL/6J – (catalog #: 000664, The Jackson Laboratory)
Wnt1-Cre2 – (catalog #: 002137, The Jackson Laboratory)
R26R-Confetti – (catalog #: 013731, The Jackson Laboratory)

Dani N., Herbst R.H., McCabe C., Green G., Kaiser K., Head J., Cui J., Shipley F.B., Jang A., Dionne D., Nguyen L., Rodman C., Riesenfeld S.J., Prochazka J., Prochazkova M., Sedlacek R., Zhang F., Bryja V., Rozenblatt-Rosen O., Habib N., Regev A, & Lehtinen M.K. (2021). A cellular and spatial map of the choroid plexus across brain ventricles and ages. Cell, 184(11), 3056-3074.e21.

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