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Rab35

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RAB35 is a member of the Rab family of small GTPases. It plays a role in vesicle trafficking and endocytosis.

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Lab products found in correlation

Pggt1b, by Fortis Life Sciences (2 mentions) Rabggta, by Merck Group (2 mentions) Rabggta, by OriGene (2 mentions) Gfp tagged cdna of rab1a, by Addgene (2 mentions) Rab7a, by Addgene (2 mentions) Rab11, by Addgene (2 mentions) Rab34, by Addgene (2 mentions) Anti ha, by Fortrea (2 mentions) Pshuttle cmv, by Addgene (1 mentions) Adeasier 1 cells, by Addgene (1 mentions) Gfp rab27a, by Addgene (1 mentions) Pvsv g, by Addgene (1 mentions) Pmdlg prre, by Addgene (1 mentions) Pspax2, by Addgene (1 mentions)

3 protocols using rab35

1

Validation of PTAR1 antibody specificity

Cited 1 time
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A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
Kuchay S., Wang H., Marzio A., Jain K., Homer H., Fehrenbacher N., Philips M.R., Zheng N, & Pagano M. (2019). GGTase3 is a Newly Identified Geranylgeranyltransferase Targeting a Ubiquitin Ligase. Nature structural & molecular biology, 26(7), 628-636.
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2

Lentiviral and Adenoviral Vector Construction

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The following constructs were procured for this study: GFP-Rab27A (gift from William Gahl; Addgene plasmid #89237); Rab35 (gift from Peter McPherson; Addgene plasmid #47424); Neo DEST (705–1) (gift from Eric Campeau & Paul Kaufman; Addgene plasmid #17392); pMDLg/pRRE (gift from Didier Trono; Addgene plasmid # 12251); pVSVG (gift from Bob Weinberg; Addgene plasmid #8454); psPAX2 (gift from Didier Trono; Addgene plasmid #12260); pShuttle-CMV (gift from Bert Vogelstein; Addgene plasmid #16403); and AdEasier-1 cells (gift from Bert Vogelstein; Addgene, #16399).
Francis C.R, & Kushner E.J. (2021). Capturing membrane trafficking events during 3D angiogenic development in vitro. Microcirculation (New York, N.y. : 1994), 29(6-7), e12726.
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3

Validation of PTAR1 antibody specificity

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A rabbit polyclonal antibody against the peptide corresponding to amino acids 339 to 359 (CDGLNDSSKQGYSQETKRLKRT) of human PTAR1 primary sequence was custom made by YenZyme antibodies, LLC, CA 94080. The specificity of the antibody was validated by silencing PTAR1 in primary fibroblasts NHFs and HEK293T cell lines (Supplementary Fig. 1A). Antibodies were from Abcam (FNTA, FNTB), Abnova (PGGT1B), Bethyl (RabGGTB), Aviva Biosystems (RabGGTA), Sigma (anti-FLAG M2, anti-FLAG, β-actin), Santa Cruz Biotechnology (SKP1), and Covance (anti-HA). A polyclonal rabbit antibody to FBXL2 was generated using a peptide against FBXL2 as previously described27 (link). Human cDNA ORFs for PTAR1, FNTA, and RabGGTA were purchased from Origene. GFP-tagged cDNA of RAB1a, RAB7a, RAB11, RAB34, and RAB35 were obtained from Addgene. The variously tagged-genes and mutants were generated in pcDNA3 and pEGFPC1 vectors either by sub-cloning or site-directed mutagenesis methodologies, as described.
Kuchay S., Wang H., Marzio A., Jain K., Homer H., Fehrenbacher N., Philips M.R., Zheng N, & Pagano M. (2019). GGTase3 is a Newly Identified Geranylgeranyltransferase Targeting a Ubiquitin Ligase. Nature structural & molecular biology, 26(7), 628-636.
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