UV-Vis spectra were recorded as previously described [21 (link)]. NMR spectra were measured on an Avance III 500 MHz spectrometer (Bruker, Bremen, Germany) and the solvents residual signals were used as internal references (DMSO-d6, at δH 2.50 and δC 39.5 ppm). The ESI-HRMS data were recorded using a Bruker microTOF-Q II mass spectrometer (Bremen, Germany). Open column chromatographies were performed with silica gel 60 (40–63 µm, HiMedia, Mumbai, India) or Sephadex LH-20 (25–100 µm) (Pharmacia Fine Chemicals, Uppsala, Sweden).
Silica gel 60
Manufactured by HiMedia
Sourced in India
Silica gel 60 is a porous, amorphous form of silicon dioxide (SiO2) commonly used in laboratory applications. It is an inert and chemically stable material with a high surface area, making it suitable for various separation and adsorption techniques. Silica gel 60 is widely used in chromatography, desiccation, and other laboratory procedures where the controlled adsorption and desorption of moisture, gases, or other substances are required.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel 60 rp 18 f254s, by Merck Group (5 mentions)
Silica gel 60 f254, by Merck Group (4 mentions)
Avance 3 500 mhz spectrometer, by Bruker (2 mentions)
Avance 3 spectrometer, by Bruker (2 mentions)
Acarbose, by Merck Group (2 mentions)
Microtof q 2 mass spectrometer, by Bruker (1 mentions)
60 rp 18 f254s, by Merck Group (1 mentions)
J 815 circular dichroism spectrometer, by Jasco (1 mentions)
Avance 3, by Bruker (1 mentions)
Avance 500 spectrometer, by Bruker (1 mentions)
P nitrophenyl α d glucopyranoside, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Advance 3, by Bruker (1 mentions)
Microtof q 2, by Bruker (1 mentions)
8 protocols using silica gel 60
1
Spectroscopic Characterization of Compounds
2
Characterization and Enzyme Inhibition Analysis
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NMR spectra were recorded on a Bruker Avance III spectrometer (500 MHz for 1H NMR and 125 MHz for 13C NMR) with TMS as internal standard. HRESIMS was recorded using a MicrOTOF-Q mass spectrometer on an LC-Agilent 1100 LC-MSD Trap spectrometer. Thin-layer chromatography (TLC) was carried out using precoated silica gel 60 F254 or 60 RP-18 F254S (Merck). Spots were visualized by applying a 10% H2SO4 solution, followed by heating. Gravity column chromatography was performed on silica gel 60 (0.040–0.063 mm, Himedia). Saccharomyces cerevisiae α-glucosidase (E.C. 3.2.1.20) and acarbose were obtained from Sigma-Aldrich Co.
3
Characterization of Organic Compounds
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ECD spectra were obtained on a JASCO J-815 circular dichroism spectrometer (JASCO, Easton, MD, USA). 1D and 2D NMR spectra were acquired on a Bruker AVANCE III 500 MHz spectrometer in acetone-d6. Chemical shifts in ppm are referenced to the residual solvent signal (acetone-d6: δH = 2.05, δC = 29.8 ppm). The HRESIMS spectra were recorded using a MicrOTOF-Q mass spectrometer on an LC-Agilent 1100 LC-MSD Trap spectrometer. Silica gel 60 (0.040–0.063 mm, Himedia) were used for column chromatography. Analytical TLC was carried out on aluminum plates precoated with Silica gel 60 F254 or Silica gel 60 RP-18 F254S (Merck), and eluted zones were visualized by spraying with 10% H2SO4 solution, followed by heating.
4
NMR and Mass Spectrometry Protocol
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The NMR spectra were measured on a Bruker Avance III (500 MHz for 1H-NMR and 125 MHz for 13C-NMR, Bruker, Bremen, Germany) spectrometer with TMS as internal standard. Chemical shifts are expressed in ppm with reference to the residual protonated solvent signals (acetone-d6 with δH 2.05, δC 206.26, and 29.84) or the internal TMS (0.00). The HR–ESI–MS were recorded on a HR–ESI–MS Bruker microOTOF Q-II (Bremen, Germany). TLC was carried out on precoated silica gel 60 F254 or silica gel 60 RP-18 F254S (Merck, Darmstadt, Germany), and spots were visualized by spraying with 10% H2SO4 solution followed by heating. Gravity column chromatography was performed with silica gel 60 (0.040–0.063 mm, Himedia, Mumbai, India).
5
NMR Spectroscopy and Chromatographic Techniques
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NMR spectra were recorded on a Bruker Avance III spectrometer (500 MHz for 1H NMR and 125 MHz for 13C NMR) using residual solvent signals as internal references: acetone‑d6 at δH 2.05, δC 29.84 and chloroform-d at δH 7.26, δC 77.18. Thin layer chromatography (TLC) was carried out on precoated silica gel 60 F254 or silica gel 60 RP–18 F254S (Merck), and spots were visualized by spraying with 10% H2SO4 solution followed by heating. Gravity column chromatography was performed on silica gel 60 (0.040–0.063 mm, Himedia).
6
Characterization of Organic Compounds
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The HR-ESI-MS was recorded on a HR-ESI-MS MicrOTOF-Q mass spectrometer. The 1 H-NMR 500 (MHz) and 13 C-NMR (125 MHz) spectra were recorded on a Bruker Avance 500 spectrometer. Thinlayer chromatography (TLC) was carried out on precoated silica gel 60 F254 or silica gel 60 RP-18 F254S (Merck), and the isolated compounds were visualized by spraying with 10% H 2 SO 4 solution followed by heating. Gravity column chromatography was performed on silica gel 60 (0.040-0.063 mm, Himedia).
7
Characterization of Glycosidase Inhibitors
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The HR-ESI-MS was recorded on an HR-ESI-MS MicrOTOF-Q mass spectrometer. The 1 H-NMR (500 MHz) and 13 (link) C-NMR (125 MHz) spectra were recorded on a Bruker Avance 500 spectrometer. Chemical shifts are expressed in ppm using a residual solvent signal as internal reference (CDCl 3 δ H 7.26, δ C 77.2). α-Glucosidase (EC 3.2.1.20) from Saccharomyces cerevisiae (750 UN) and p-nitrophenyl-α-Dglucopyranoside were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Acarbose and dimethyl sulfoxide were obtained from Merck. Other chemicals were of the highest grade available.
Thin-layer chromatography (TLC) was carried out on precoated silica gel 60 F254 or silica gel 60 RP-18 F254S (Merck) and the isolated compounds were visualized by spraying with 5% vanillin solution followed by heating. Gravity column chromatography was performed on silica gel 60 (0.040-0.063 mm, Himedia).
Thin-layer chromatography (TLC) was carried out on precoated silica gel 60 F254 or silica gel 60 RP-18 F254S (Merck) and the isolated compounds were visualized by spraying with 5% vanillin solution followed by heating. Gravity column chromatography was performed on silica gel 60 (0.040-0.063 mm, Himedia).
8
Isolation and Characterization of (+)-Usnic Acid
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(+)-Usnic acid isolated from lichen. Acetyl chloride, benzoyl chloride (Sigma-Aldrich). Silica gel 60 (HiMedia, India). Bruker Advance III (400 MHz for 1 H NMR and 100 MHz for 13 C NMR) spectrometer with TMS as internal standard recorded NMR spectra. The HR-ESI-MS were recorded on a HR-ESI-MS Bruker microTOF Q-II. Column chromatography was performed with Silica gel 60.
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