Clone pck 26

Tumor tissues were harvested from mice injected with MOE/E6E7^Vector and MOE/E6E7^CXCL14 cells and fixed overnight in 4% paraformaldehyde. All tissues were cryoprotected using 20% sucrose in PBS and subsequently frozen in optimal cutting temperature (OCT) compound. Tissue was cryosectioned at 12-μm increments. Each tissue section was blocked using lamb serum and permeabilized using triton X. Immunofluorescence was performed on the tissue sections using the following antibodies: mouse anti-pan cytokeratin (1:100, Novus, clone PCK-26, Cat# NB120–6401) and rat anti-CD8α (1:200, Novus, clone 53–6.7, Cat# NBP1–49045) antibodies. After incubation with primary antibodies, sections were incubated with appropriate Alexa Fluor–conjugated secondary antibodies: anti-mouse IgG Alexa Fluor 488 (Invitrogen, Cat# A-11029), anti-rat IgG Alexa Fluor 594 (Invitrogen, Cat# A-21209), and DAPI (Invitrogen). Immunofluorescence images were captured using a Zeiss 780 LSM confocal microscope.