A monoclonal anti β actin antibody

βBA, obtained from Sigma-Aldrich (St. Louis, MO, USA), was dissolved and prepared with 5, 10, 20, and 30 mM stock solutions in DMSO. The control group was added 0.1% DMSO. TRIzol reagent was obtained from Life Technologies (Carlsbad, CA, USA). A monoclonal anti-β-actin antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against phospho-Akt, anti-Akt, anti-phospho-p38, anti-total p38, anti-phospho-IκB, anti-Bruton’s tyrosine kinase, and anti-phospho-PLCγ2 were obtained from Cell Signaling Technology Inc. (Beverly, MA, USA). Anti-phospho-Btk antibody was obtained from GeneTex (Irvine, CA, USA). Anti-c-Fos, anti-NFATc1, anti-IκB, and anti-PLCγ2 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Donkey anti-rabbit and anti-mouse immunoglobulin secondary antibodies were purchased from Enzo Life Sciences (Farmingdale, NY, USA).

Antibodies against phosphorylated-cAMP response element binding (CREB) protein (p-CREB; Ser133), and CREB (48H2) were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA); against p-extracellular signal-regulated kinases (ERK) 1/2 (Thr202/Tyr204), ERK1/2 (MK1) and BDNF (N-20) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A monoclonal anti-β-actin antibody, methylthiazolyldiphenyl-tetrazolium bromide (MTT) and melatonin were provided by Sigma-Aldrich (St. Louis, MO, USA).