Deeply anesthetized animals underwent transcardial systemic perfusion with 0.1 Msodium cacodylate buffer (pH 7.2), followed by 10–20 ml of 3% glutaraldehyde + 3 mM CaCl2 in 0.1 M sodium cacodylate buffer (pH7.2). Dissected utricles and saccules were then immersion-fixed for 24 hours in the same fixative, washed, and post-fixed in 1% osmium tetroxide for 1 hour. Samples were then dehydrated, critical point dried in CO2, mounted, sputter coated with gold palladium and viewed on a Jeol (JSM-848) scanning electron microscope at 15 kV.
Jsm 848
Manufactured by JEOL
The JSM-848 is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution images of small-scale samples. The JSM-848 utilizes an electron beam to scan the surface of a specimen and produces an image based on the interactions between the electrons and the sample.
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Lab products found in correlation
2 protocols using jsm 848
1
Scanning Electron Microscopy of Inner Ear
2
Tantalite Ore Leaching and Characterization
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Employing an Anton Paar PerkinElmer Multiwave 3000 microwave reaction system fitted with an 8SXF100 rotor and eight PTFE reaction vessels, a concentrated tantalite sample was leached using a solution of 6 M HF and 1 M H2SO4. TGA was used to analyze the weight fraction, weight loss, and thermal stability of tantalite ore samples as their temperature increases. Model TA 50, Shimadzu America, and platinum cell in nitrogen with a flow rate of 30 mL/min were the instruments used. SEM with EDS (JEOL JSM-848 model) was used to examine the compositional and topographic changes that occurred both before and after the samples were leached. The dissolution procedure was conducted in a stainless batch reactor (SUS316) within a high-temperature furnace set at different temperatures of 100 -400 °C. No additional purification was required when using research-grade HF, H2SO4, ammonia solution, and methyl isobutyl ketone (MIBK) from Sigma-Aldrich.
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