Log In Sign up
The largest database of trusted experimental protocols

Anti cd40l mr1

Manufactured by BioXCell
Visit Supplier

Anti-CD40L (MR1) is a monoclonal antibody that binds to the CD40 ligand (CD40L) protein. CD40L is a costimulatory molecule involved in various immune responses. The primary function of Anti-CD40L (MR1) is to bind and neutralize CD40L.

Automatically generated - may contain errors

Lab products found in correlation

Control hamster igg, by BioXCell (2 mentions) Tamoxifen, by Merck Group (1 mentions) Armenian hamster igg, by BioXCell (1 mentions) Abatacept, by Bristol-Myers Squibb (1 mentions) Mp5 20f3, by BioXCell (1 mentions) Bzn035, by Novartis (1 mentions)

Spelling variants of this product

Anti-CD40L (11 citations) CD40L (4 citations) Anti-CD40L mAb (clone MR1, (4 citations) Anti-CD40L mAb (MR1) (2 citations) Anti-CD40L antibody (clone MR1, (2 citations)

7 protocols using anti cd40l mr1

1

Germinal Center Reaction Blockade

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce primary responses 2.5 × 104 HEL-binding B cells from tamoxifen-treated SWHEL/Sykfl/fl or SWHEL/Sykfl/flRMCM mice were transferred into B6.SJL recipient mice together with 5 × 108 HEL-conjugated sheep RBCs (SRBC). To generate a memory pool, splenocytes containing an equivalent of 5 × 105 HEL-binding B cells from SWHEL/Sykfl/+RMCM or SWHEL/Sykfl/flRMCM mice were transferred into (B6 x B6.SJL)F1 recipient mice together with 1 × 109 HEL3×-conjugated SRBC. HEL (Sigma-Aldrich) or HEL3× (a gift from R. Brink) (17 (link)) was conjugated to SRBC (Patricell) as described previously (18 ). The germinal center reaction was blocked by three injections of 300 μg anti-CD40L (MR1) or control Hamster IgG (BioXcell) every second day.
Ackermann J.A., Nys J., Schweighoffer E., McCleary S., Smithers N, & Tybulewicz V.L. (2015). Syk Tyrosine Kinase Is Critical for B Cell Antibody Responses and Memory B Cell Survival. The Journal of Immunology Author Choice, 194(10), 4650-4656.
+ Open protocol
+ Expand
2

Anti-CD40L and BAFF Depletion in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
To block ongoing GC responses, immunized mice were injected i.p. on 3 alternate d with 300 µg anti-CD40L (MR-1; BioXCell). To induce Cre recombinase activity, 2 mg of tamoxifen (20 mg/ml in corn oil; Sigma Aldrich) was administered i.p. daily for 5 d. BAFF depletion in mice was achieved by two i.p. injections of 100 µg anti-BAFF (10F4; GSK) or, as a control, with Armenian hamster IgG (BioXCell).
Müller-Winkler J., Mitter R., Rappe J.C., Vanes L., Schweighoffer E., Mohammadi H., Wack A, & Tybulewicz V.L. (2020). Critical requirement for BCR, BAFF, and BAFFR in memory B cell survival. The Journal of Experimental Medicine, 218(2), e20191393.
+ Open protocol
+ Expand
3

Bone Marrow and Heterotopic Heart Transplant

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow and heterotopic heart transplantation were performed as described previously.12 (link)-14 (link) Briefly, bone marrow cell suspension collected from femurs and vertebraes were passed through a 70 μm mesh. After blood cell lysis, 15 × 106 bone marrow cells were co-injected with 3 × 106 ortho Tregs into recipient mice prepared with 3.3-Gy total body irradiation (TBI). Anti-CD40L (MR1; BioX-cell) was diluted with PBS and injected i.p. at 0.3 mg/mouse right after BMC transfer. The newborn heart was excised and placed into the recipient's ear pinna through a tunnel made from a small incision in the occipital skin. Heart graft viability was assessed with visual observation. The rejection of the graft was confirmed by histopathology at the end of the experiment.
Jensen J.S., Bruun B, & Gahrn-Hansen B. (1999). Unexpected Cross-Reaction with Fusobacterium necrophorum in a PCR for Detection of Mycoplasmas. Journal of Clinical Microbiology, 37(3), 828-829.
+ Open protocol
+ Expand
4

Inducing Primary and Memory B Cell Responses

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce primary responses 2.5 × 104 HEL-binding B cells from tamoxifen-treated SWHEL/Sykfl/fl or SWHEL/Sykfl/flRMCM mice were transferred into B6.SJL recipient mice together with 5×108 HEL-conjugated sheep red blood cells (SRBC). To generate a memory pool, splenocytes, containing an equivalent of 5 × 105 HEL-binding B cells from SWHEL/Sykfl/+RMCM or SWHEL/Sykfl/flRMCM mice were transferred into (B6 × B6.SJL)F1 recipient mice together with 1×109 HEL3X-conjugated SRBC. HEL (Sigma-Aldrich) or HEL3X (gift from R. Brink) (17 (link)) was conjugated to SRBC (Patricell) as described (18 ). The germinal center reaction was blocked by three injections of 300 μg anti-CD40L (MR1) or control Hamster IgG (BioXcell) every second day.
Ackermann J.A., Nys J., Schweighoffer E., McCleary S., Smithers N, & Tybulewicz V.L. (2015). Syk Tyrosine Kinase Is Critical for B Cell Antibody Responses and Memory B Cell Survival. The Journal of Immunology Author Choice, 194(10), 4650-4656.
+ Open protocol
+ Expand
5

NP-KLH Immunization and GC Ablation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary immunizations were performed with 400μg 4-hydroxy-3-nitrophenylacetyl (Biosearch) conjugated to keyhole limpet hemocyanin (Pierce, NP-KLH) mixed with MPL-based adjuvant subcutaneously at the base of the tail. Secondary immunizations (boosts) were subcutaneous or i.v. with 100μg NP-KLH in the absence (PBS only, soluble) or presence of MPL-based adjuvant greater than 70 days after priming. Immunization with Alum adjuvant (Aluminum-potassium-sulfate (Sigma) precipitated with 1M potassium hydroxide (Sigma)) and Complete Freund's Adjuvant (CFA, Sigma) was used as indicated.
To ablate persisting GCs in primed animals, anti-CD40L (MR1, BioXCell) blocking antibody was injected 3 times every other day (300μg per injection, i.p.) starting on week 9 after priming. Mice were sacrificed or boosted 6 days after the last injection. Matching doses of polyclonal hamster IgG (Pierce) were used as control.
McHeyzer-Williams L.J., Milpied P.J., Okitsu S.L, & McHeyzer-Williams M.G. (2015). Switched-memory B cells remodel B cell receptors within secondary germinal centers. Nature immunology, 16(3), 296-305.
+ Open protocol
+ Expand
6

Tolerance Induction by Anti-CD40L, CTLA4-Ig, and Rapamycin

Cited 4 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Groups of age-matched C57BL/6 recipients received 20 × 106 unseparated BM cells or splenocytes [as donor-specific transfusion (DST) control] from BALB/c donors by injection into the tail vein together with CB consisting of anti-CD154 mAb (1 mg, d0, anti-CD40L, MR1, BioXcell), hCTLA4-Ig (0.5 mg, d2, abatacept, Bristol Myers Squibb), and a short-course of rapamycin (0.1 mg, d-1, d0, and d2, LC Laboratories) (6 (link)). Indicated recipients were additionally treated with an anti-IL-6 mAb (1 mg on days −1, 1, and 3 and then 0.1 mg every other day until day 13, MP5-20F3, BioXcell) (27 (link), 28 (link)). Where indicated, groups of mice received in addition (0.5 mg on days −1, 1, and 3 and 0.1 mg every other day until day 13) anti-IL-6R (15A7) or anti-IL-17A (BZN035, Novartis Pharma AG) (42 (link), 43 (link)).
Granofszky N., Farkas A.M., Muckenhuber M., Mahr B., Unger L., Maschke S., Pilat N., Holly R., Wiletel M., Regele H, & Wekerle T. (2017). Anti-Interleukin-6 Promotes Allogeneic Bone Marrow Engraftment and Prolonged Graft Survival in an Irradiation-Free Murine Transplant Model. Frontiers in Immunology, 8, 821.
+ Open protocol
+ Expand
7

NP-KLH Immunization and GC Ablation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary immunizations were performed with 400μg 4-hydroxy-3-nitrophenylacetyl (Biosearch) conjugated to keyhole limpet hemocyanin (Pierce, NP-KLH) mixed with MPL-based adjuvant subcutaneously at the base of the tail. Secondary immunizations (boosts) were subcutaneous or i.v. with 100μg NP-KLH in the absence (PBS only, soluble) or presence of MPL-based adjuvant greater than 70 days after priming. Immunization with Alum adjuvant (Aluminum-potassium-sulfate (Sigma) precipitated with 1M potassium hydroxide (Sigma)) and Complete Freund's Adjuvant (CFA, Sigma) was used as indicated.
To ablate persisting GCs in primed animals, anti-CD40L (MR1, BioXCell) blocking antibody was injected 3 times every other day (300μg per injection, i.p.) starting on week 9 after priming. Mice were sacrificed or boosted 6 days after the last injection. Matching doses of polyclonal hamster IgG (Pierce) were used as control.
McHeyzer-Williams L.J., Milpied P.J., Okitsu S.L, & McHeyzer-Williams M.G. (2015). Switched-memory B cells remodel B cell receptors within secondary germinal centers. Nature immunology, 16(3), 296-305.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!