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4 protocols using potassium peroxodisulfate

1

Synthesis of Graphene Oxide from Graphite Nanofibres

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By the modified Hummers method, nGO was prepared using platelet graphite nanofibres (PGNFs) as the starting material [13 (link)]. The PGNF (diameter: 50 nm) was purchased from Strem Chemicals, Inc. Potassium peroxo-disulfate and phosphorus pentoxide were purchased from Nacalai Tesque, Inc. Sorbitan monooleate (Span 80), ethylenediamine (EDA) and 2, 2′-oxybisethylamine (OBEA) were purchased from Tokyo Chemical Industry Co., Ltd. Methyl orange was purchased from FUJIFILM Wako Pure Chemical Corp. The purchased chemicals were used without further purification.
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2

Antioxidant and Anti-Gout Potential Evaluation

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Potassium phosphate monobasic and dibasic, Folin-Ciocalteu’s phenol, xanthine oxidase, xanthine, allopurinol, dimethyl sulfoxide (DMSO), hydrochloric acid, tricholoroacetic acid (TCA), Tween 20, thiobarbituric acid (TBA), proline, sulfosalicylic acid, glacial acetic acid, ninhydrin, and toluene were obtained from Sigma-Aldrich Japan K.K. (Tokyo, Japan). Sodium acetate, acetic acid, aluminium (III) chloride hexahydrate, 1,1-diphenyl-2-picrylhydrazyl (DPPH), dibutyl hydroxytoluene (BHT), and gallic acid were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Acetone, potassium peroxodisulfate, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). Methanol and ethanol at analytical grade were obtained from Junsei Chemical Co.; Ltd. (Tokyo, Japan).
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3

Antioxidant and Antimicrobial Assays

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Folin-Ciocalteu’s phenol, potassium phosphate monobasic and dibasic, xanthine, xanthine oxidase, allopurinol, and hydrochloric acid were obtained from Sigma-Aldrich Japan K.K., Tokyo, Japan. Aluminium (III) chloride hexahydrate, 1,1-diphenyl-2-picrylhydrazyl (DPPH), sodium acetate, acetic acid, dibutyl hydroxuytoluene (BHT), gallic acid, and quercetin were purchased from Kanto Chemical Co. Inc., Tokyo, Japan. Acetone, potassium peroxodisulfate, 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were obtained from Nacalai Tesque, Inc., Kyoto, Japan. Methanol (MeOH), ethyl acetate (EtOAc), ethanol, and methanol plus were obtained from Junsei Chemical Co., Ltd., Tokyo, Japan. All of the bacteria strains, including Escherichia coli, Proteus mirabilis, Staphylococcus aureus, and Bacillus subtilis, were purchased from Sigma-Aldrich (Tokyo, Japan).
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4

Antioxidant Activity of Naphtha-lene Derivatives

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Naphthalene, 1-Nap, ABTS, DPPH, potassium peroxodisulfate, phosphoric acid were products of Nacalai Tesque Inc. (Kyoto, Japan). 1-NapS was from Research Organics Inc. (Cleveland, OH, USA). 1-Methoxynaph-thalene (1-NapM), 2-Nap, and potassium dichromate were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2-NapS was a product of Alfa Aesar (Ward Hill, MA, USA). 2-Methoxynaphtalene (2-NapM) and CPZ were products of Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) was from Sigma-Aldrich, Co. (St Louis, MO, USA). All other chemicals were of the highest grade commercially available. Figure 1 shows the chemical structures of Nap isomers and their derivatives used in this study.
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