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Alexafluor conjugated secondary antibodies 488 555 633 647 conjugated anti mouse rabbit goat chicken and rat antibodies

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AlexaFluor-conjugated secondary antibodies are fluorescently-labeled antibodies that bind to the primary antibodies used in various immunodetection techniques. They are available in different fluorescent dye conjugates (488, 555, 633, 647) and can target mouse, rabbit, goat, chicken, and rat primary antibodies.

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Lab products found in correlation

Laminin l2020, by Merck Group (2 mentions) Bovine plasma fibronectin 341631, by Merck Group (2 mentions) Collagen fromcalf skin c8919, by Merck Group (2 mentions) Poly l ornithine solution p4957, by Merck Group (2 mentions) G5169 ggti 298 trifluoroacetate salt hydrate, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (2 mentions) Phalloidin atto 647n, by Merck Group (2 mentions) Prolong diamond antifade mountant with dapi, by Thermo Fisher Scientific (2 mentions) Laminin 521, by BioLamina (2 mentions)

Close spelling variants of this product

AlexaFluor 488 and 647-conjugated secondary antibodies (8 citations) 633‐conjugated anti‐mouse and anti‐rabbit secondary antibodies (2 citations) AlexaFluor 488 goat anti-mouse/AlexaFluor 555 goat anti-rabbit (2 citations) AlexaFluor 647 goat anti-mouse secondary antibodies (2 citations) Alexafluor 488 goat anti-mouse secondary antibodies (2 citations) Alexafluor 488-conjugated goat-anti rabbit antibodies (3 citations) Secondary AlexaFluor goat anti-mouse and anti-rabbit (2 citations) AlexaFluor 488/555/633 (3 citations) Alexafluor goat anti-chicken 488 (2 citations) Chicken anti-rabbit AlexaFluor 488 (2 citations)

2 protocols using alexafluor conjugated secondary antibodies 488 555 633 647 conjugated anti mouse rabbit goat chicken and rat antibodies

1

Antibody-based Immunofluorescence Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in the study are described in Supplementary Table 2.
Phalloidin–Atto 647N (65906, Sigma-Aldrich, 1:400) and Alexa
Fluor® 488 Phalloidin (A12379, Life Technologies, 1:100) were used to
stain filamentous actin. AlexaFluor-conjugated secondary antibodies (488, 555,
633, 647-conjugated anti-mouse, rabbit, goat, chicken and rat antibodies, Life
Technologies) were used in immunofluorescence (1:300 or 1:1000 (anti-chicken))
and FACS (1:200). The 4′6-diamidino-2-phenylindole (DAPI, Life
Technologies 1:10000) or DAPI-containing mounting medium (ProLong Diamond
Antifade Mountant with DAPI, Thermo Fisher) were used for nuclear staining. The
bovine plasma fibronectin (341631) was obtained from Merck. The collagen from
calf skin (C8919), poly-L-Ornithine solution (P4957) and laminin (L2020) were obtained
from Sigma-Aldrich. The laminin-521 was obtained from BioLamina. Rap1 inhibitor,
G5169-GGTI 298 trifluoroacetate salt hydrate (Sigma-Aldrich), was dissolved in
sterile DMSO.
Lilja J., Zacharchenko T., Georgiadou M., Jacquemet G., De Franceschi N., Peuhu E., Hamidi H., Pouwels J., Martens V., Nia F.H., Beifuss M., Boeckers T., Kreienkamp H.J., Barsukov I.L, & Ivaska J. (2017). SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras. Nature cell biology, 19(4), 292-305.
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2

Antibody-based Immunofluorescence Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in the study are described in Supplementary Table 2.
Phalloidin–Atto 647N (65906, Sigma-Aldrich, 1:400) and Alexa
Fluor® 488 Phalloidin (A12379, Life Technologies, 1:100) were used to
stain filamentous actin. AlexaFluor-conjugated secondary antibodies (488, 555,
633, 647-conjugated anti-mouse, rabbit, goat, chicken and rat antibodies, Life
Technologies) were used in immunofluorescence (1:300 or 1:1000 (anti-chicken))
and FACS (1:200). The 4′6-diamidino-2-phenylindole (DAPI, Life
Technologies 1:10000) or DAPI-containing mounting medium (ProLong Diamond
Antifade Mountant with DAPI, Thermo Fisher) were used for nuclear staining. The
bovine plasma fibronectin (341631) was obtained from Merck. The collagen from
calf skin (C8919), poly-L-Ornithine solution (P4957) and laminin (L2020) were obtained
from Sigma-Aldrich. The laminin-521 was obtained from BioLamina. Rap1 inhibitor,
G5169-GGTI 298 trifluoroacetate salt hydrate (Sigma-Aldrich), was dissolved in
sterile DMSO.
Lilja J., Zacharchenko T., Georgiadou M., Jacquemet G., De Franceschi N., Peuhu E., Hamidi H., Pouwels J., Martens V., Nia F.H., Beifuss M., Boeckers T., Kreienkamp H.J., Barsukov I.L, & Ivaska J. (2017). SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras. Nature cell biology, 19(4), 292-305.
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