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2 protocols using zinc nitrate hexahydrate

1

Quantification of Mercury Ions Using Thymine

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All chemicals were purchased and used without any further purification. To perform mercury ion quantification assay, mercury nitrate (99%, Guangzhou chemical reagent factory, Guangzhou, China) was dissolved in purified water to prepare aqueous solution with various concentrations. Thymine (Sigma-Aldrich Co., LLC., St. Louis, MO, USA) was dissolved in purified water to prepare solutions of 10−3 M. Cetyltrimethyl-ammonium bromide (CTAB, 99%), cadmium chloride (CdCl2, 99%) obtained from Kermel (Tianjin, China), toluene (C7H8, A.R.), hydrochloric acid (37% HCl, A.R.), Ethanol (C2H5OH, A.R.), potassium nitrate (KNO3, 99%), zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), cobaltous nitrate gexahydrate (Co(NO3)2·6H2O, 99%), copper nitrate hydrate (Cu(NO3)2·3H2O, 99%) and iron nitrate nonahydrate (Fe(NO3)3·9H2O, 99%) obtained from Guangzhou chemical reagent factory (Guangzhou, China). Hydrogen tetrachloroaurate (HAuCl4 3H2O, 99.99%), sodium borohydride (NaBH4, 99.99%), silver nitrate (AgNO3, 99.99%) and ascorbic acid (AA, 98%) were purchased from Sigma-Aldrich (Sigma-Aldrich Co., LLC., St. Louis, MO, USA). Sodium nitrate (NaNO3, 99%) was purchased from Damao chemical reagent company (Tianjin, China). Millipore water was used through all the experiments.
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2

Antimicrobial Nanomaterial Synthesis and Evaluation

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Zinc nitrate hexahydrate (ZnNO3·6H2O, 99%), ethanol (CH3CH3O, 99.7%) and methanol (CH3OH, 99.5%) were purchased from Guang Zhou Chemical Reagent Factory. 2-Methylimidazole (2-MIM, 98%) was purchased from Macklin. Curcumin (CCM, 98%) and indocyanine green (ICG, 75%) were purchased from Aladdin. Bacterial culture media was purchased from Huan Kai Microbial. Gram-positive bacteria (S. aureus, ATCC 23235) and Gram-negative bacteria (E. coli, ATCC 43894) were obtained from China Center of Industrial Culture. LIVE/DEAD Bac Light Bacterial Viability Kit was purchased from Thermo Fisher Scientific.
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