Atomic force microscopy (AFM) studies were performed to determine the size and surface morphology of synthesized AgNPs on a "Solver Pro" microscope (NT-MDT Spectrum Instruments, Moscow, Russia), equipped with an optical microscope. Measurements were carried out in tapping mode using AFM probes RTESPA (Bruker, Mannheim, Germany, 300 kHz, 40 N/m). A freshly cleaved atomically smooth surface of mica (V1 grade, SPI Supplies) was used as a substrate. A droplet of suspension (0.2 µL) containing nanoparticles (naked AgNPs, AgNP-TBA, and AgNP-MUA) was applied onto a limited area of the mica and left until complete evaporation of the water. The optimal measurement concentrations of AgNPs were achieved by a dilution of stock solutions with distilled water.
Rtespa
Manufactured by Bruker
Sourced in United States
The RTESPA is a laboratory equipment product from Bruker. It is designed for conducting various analytical and research tasks. The core function of the RTESPA is to provide a platform for sample analysis and data collection, without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
8 protocols using rtespa
1
Atomic Force Microscopy of Silver Nanoparticles
2
Atomic Force Microscopy of Metastatic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eighteen fixed histological tissues (eleven metastatic and seven non-metastatic) were imaged with Innova AFM (Bruker/Veeco, Inc., Santa Barbara, CA, USA) operating in tapping mode with a phosphorus (n)-doped silicon cantilever (RTESPA, Bruker, Madison, WI, USA) with a nominal tip diameter of 8–10 nm and a nominal spring constant of 40 N/m at a 300 kHz resonance frequency.
Surface image quality was optimised by lowering the scan rate to 0.2 Hz. All images were acquired with 50 μm × 50 μm scan sizes, 512 × 512 data point resolution, and a pixel size of 97.7 nm. In addition to height, amplitude and phase images were also recorded. The AFM was installed on a vibration isolation table (minus k technology BM-10, Inglewood, CA, USA) to compensate for regular environmental vibrations and placed inside an acoustic enclosure (Ambios technologies Isochamber, Santa Cruz, CA, USA) for thermal and building vibration isolation. The AFM imaging was performed in air at a constant ambient temperature.
Surface image quality was optimised by lowering the scan rate to 0.2 Hz. All images were acquired with 50 μm × 50 μm scan sizes, 512 × 512 data point resolution, and a pixel size of 97.7 nm. In addition to height, amplitude and phase images were also recorded. The AFM was installed on a vibration isolation table (minus k technology BM-10, Inglewood, CA, USA) to compensate for regular environmental vibrations and placed inside an acoustic enclosure (Ambios technologies Isochamber, Santa Cruz, CA, USA) for thermal and building vibration isolation. The AFM imaging was performed in air at a constant ambient temperature.
3
Nanowizard AFM Characterization of Violin Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Nanowizard 1 AFM (JPK, Bruker, Ettlingen, Germany) was used to measure the Young’s Modulus of the model violin samples using a standard force-distance curve approach. The cantilever used for the measurements had a spring stiffness of k = 38 N/m (RTESPA, Bruker, Camarillo, CA, USA) with a setpoint of 500 nN. Samples were adhered to a glass slide using double-sided tape, with the varnish side exposed. Force-distance curves (300) were obtained across three separate areas of 50 µm2 using a Nanowizard (JPK, Berlin, Germany) in contact mode in the air. All FD curves were processed with the Hertzian Model on the proprietary JPK analysis RampDesigner™ software (Budapest, Hungary).
4
Atomic Force Microscopy of Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fixed histological tissues were imaged by Innova AFM (www.bruker.com ) operating in tapping mode with phosphorus (n)-doped silicon cantilever (RTESPA, Bruker, Madison, 120 WI, USA) with a nominal tip diameter of 8–10 nm, and nominal spring constant of 40 N/m at 300 kHz resonance frequency. Surface image quality was optimized by lowering the scan rate at 0.2 Hz. All images were acquired with 50 × 50 μm2 scan sizes, 512 × 512 data point resolution, and with a pixel size of 97.65 nm. Each scanned sample contains a few CRCs since 11 μm is their median size [47 (link)]. The AFM was installed on a vibration isolation table (minus k technology BM-10) to compensate for regular environmental vibrations and placed inside an acoustic enclosure (Ambios technologies Isochamber) for isolation from thermal and building vibrations and 30 dB acoustic drift. The AFM imaging was performed in air at ambient temperature. In addition to height, the amplitude and phase images were also recorded.
5
Nanoscale Imaging of Symptomatic and Asymptomatic Carotids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Images of symptomatic and asymptomatic carotids, as well as symptomatic and asymptomatic carotids with the addition of the G0 and G0/ZnPc conjugates were acquired under ambient conditions by using an AFM (d’Innova, Bruker, Madison, WI, USA). AFM provides high-resolution imaging and measurements of surface topography and properties at the nanoscale. The AFM images of symptomatic/asymptomatic carotids were acquired in tapping-mode using a phosphorus-(n)-doped silicon cantilever (RTESPA, Bruker, Madison, WI, USA) with a nominal spring constant of 40 N/m at approximately 300 kHz resonance frequency and a nominal radius of 8 nm (Figure 3 ). The AFM images were obtained at different scanning areas at a maximum scanning rate of 0.5 Hz with an image resolution of 512 × 512 pixels. Imaging was carried out at different scales from 1 to 5 μm to verify the consistency and robustness of the evaluated structures. The AFM data were processed with WSXM™ free software [53 (link)].![]()
3D-AFM image of atheromatous plaque.
6
Hydrophobicity and Mechanical Characterization of Electrospun Fibers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hydrophobic/hydrophilic nature of the electrospun fibers was measured by contact angle water droplet measurements using a CAM-PLUS contact angle meter (ChemInstruments, Fairfield, OH, USA). These measurements were repeated three times on each scaffold.
The mechanical properties of polymer fiber mats were measured with atomic force microscopy (AFM) (BioScope Catalyst; Bruker, Bilerca, MA, USA). All measurements were taken in Peak Force QNM imaging mode. Polymer fiber mats were characterized using spherical borosilicate cantilevers with spring constants of 5.6 N/m, radius of 12 μm, and resonant frequency of 300 kHz (RTESPA, Bruker). Spring constant and tip radius were calibrated on a polydimethylsiloxane (PDMS) reference sample having a known Young’s Modulus of 3.5 MPa. Average Young’s modulus measurements of the fiber mats were taken from 3 separate samples from 4 different sections each. All measurements were performed on dry samples.
The mechanical properties of polymer fiber mats were measured with atomic force microscopy (AFM) (BioScope Catalyst; Bruker, Bilerca, MA, USA). All measurements were taken in Peak Force QNM imaging mode. Polymer fiber mats were characterized using spherical borosilicate cantilevers with spring constants of 5.6 N/m, radius of 12 μm, and resonant frequency of 300 kHz (RTESPA, Bruker). Spring constant and tip radius were calibrated on a polydimethylsiloxane (PDMS) reference sample having a known Young’s Modulus of 3.5 MPa. Average Young’s modulus measurements of the fiber mats were taken from 3 separate samples from 4 different sections each. All measurements were performed on dry samples.
7
Evaluating Collagen Fibril Structure
8
Characterization of Nanomaterial Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following materials and equipment were used in this study: deionized water purified with a Milli-Q A10 system, silicon dioxide (Lijing, LLC, China), polystyrene beads (average MW ≈350,000; Aldrich, USA), AFM cantilevers (RTESPA, DNP, SNL; Bruker, USA), AFM (Innova; Bruker, USA), digital microscope (KH1300; Hirox, Japan), spin coater (KW-4A; SETCAS Electronics Co. Ltd., Beijing, China), and drop meter (MAIST, Vision, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!