Algal cells were centrifuged at 3800 g for 5 min and washed with distilled water three times. Acetone was used to extract pigments until cells were colorless. Pigments in extracts were filtered through a 0.22 μm Millipore organic membrane and then analyzed with an Agilent Ultra-Performance liquid chromatography (UPLC) 1290 Infinity. An agilent Eclipse plus C18 RRHD 1.8 μm column (2.1 × 50 mm) was used for the mobile phase, including elution A (20% water, 60% acetonitrile, 5% isopropanol and 15% methanol) and elution B (80% acetonitrile, 5% isopropanol and 15% methanol). The flow rate was 0.5 mL/min as the process blew: 100% A for 1 min followed by 100% A to 100% B with liner gradient in 1 min, and then 100% B for 6 min. The concentrations of zeaxanthin and lutein were measured according to the method reported by Huang et al. (2018) (link). Individual carotenoids were identified and quantitated through comparison to standard carotenoid retention times, absorption spectra, standard curves and peak areas.
Eclipse plus c18 rrhd 1.8 μm column
Manufactured by Agilent Technologies
The Eclipse plus C18 RRHD 1.8 μm column is a high-performance liquid chromatography (HPLC) column designed for rapid separation and analysis of a wide range of compounds. It features a 1.8 μm particle size and a C18 stationary phase for enhanced resolution and speed.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using eclipse plus c18 rrhd 1.8 μm column
1
Algal Carotenoid Extraction and Analysis
2
Carotenoids Analysis in Microalgae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carotenoids analysis was performed as described previously [27 (link)]. Briefly, 1 mL cells were harvested by centrifugation and the cell pellets were used for the extraction of pigments. The cells were extracted with acetone until the color of the sample faded.
The extracts were filtered through a 0.22 μm Millipore organic membrane, and then analyzed by an Agilent Ultra High Performance Liquid Chromatography (UHPLC) 1290 Infinity, which is equipped with an Agilent Eclipse plus C18 RRHD 1.8 μm column (2.1 × 50 mm). The mobile phase consisted of elution A (20% water, 60% acetonitrile, 5% isopropanol and 15% methanol) and elution B (80% acetonitrile, 5% isopropanol and 15% methanol). The extracts were eluted at a flow rate of 0.5 mL/min with following process: 100% A for 1 min, and then a liner gradient from 100% A to 100% B within 1 min, followed by 100% B for 8 min. Individual pigment was identified by comparing the retention times and absorption spectra to standard carotenoids, and the concentrations of pigments were determined by standard curve and peak areas (Fig. S2).
The extracts were filtered through a 0.22 μm Millipore organic membrane, and then analyzed by an Agilent Ultra High Performance Liquid Chromatography (UHPLC) 1290 Infinity, which is equipped with an Agilent Eclipse plus C18 RRHD 1.8 μm column (2.1 × 50 mm). The mobile phase consisted of elution A (20% water, 60% acetonitrile, 5% isopropanol and 15% methanol) and elution B (80% acetonitrile, 5% isopropanol and 15% methanol). The extracts were eluted at a flow rate of 0.5 mL/min with following process: 100% A for 1 min, and then a liner gradient from 100% A to 100% B within 1 min, followed by 100% B for 8 min. Individual pigment was identified by comparing the retention times and absorption spectra to standard carotenoids, and the concentrations of pigments were determined by standard curve and peak areas (Fig. S2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!