Cy3 labeled anti rat igg antibody

Manufactured by Merck Group

Sourced in United Kingdom

The CY3-labeled anti-rat IgG antibody is a laboratory reagent used for detecting and quantifying rat immunoglobulin G (IgG) in various biological samples. The antibody is conjugated with the fluorescent dye CY3, which allows for the visualization and localization of rat IgG in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Confocal laser scanning microscope, by Leica camera (2 mentions) Neun antibody, by Merck Group (2 mentions) Fluorescein labeled anti mouse antibody, by Merck Group (2 mentions) Fitc labeled anti mouse antibody, by Merck Group (2 mentions) Rat monoclonal anti brdu antibody, by Abcam (1 mentions) Mouse monoclonal anti gfap antibody, by Merck Group (1 mentions) Rat anti brdu antibody, by Abcam (1 mentions) Mouse anti gfap antibody, by Merck Group (1 mentions)

2 protocols using cy3 labeled anti rat igg antibody

Multifaceted Cellular Immunolabeling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sections were incubated with rat monoclonal anti-BrdU antibody (1:200; Abcam, Cambridge, UK), which was revealed using CY3-labeled anti-rat IgG antibody (1:200) and mouse monoclonal anti-neuronal nuclei (NeuN) antibody (1:500, Millipore), which was revealed using fluorescein-labeled anti-mouse antibody (1:50) or mouse monoclonal anti-GFAP antibody (1:200, Millipore), which was revealed using FITC-labeled anti-mouse antibody (1:50). The immunopositive cells/fibers were observed by a confocal laser-scanning microscope (Leica, Heidelberg, Germany).

Li G., Zhou L., Zhu Y., Wang C., Sha S., Xian X., Ji Y., Liu G, & Chen L. (2015). Seipin knockout in mice impairs stem cell proliferation and progenitor cell differentiation in the adult hippocampal dentate gyrus via reduced levels of PPARγ. Disease Models & Mechanisms, 8(12), 1615-1624.

+ Open protocol

+ Expand

Quantifying Neurogenesis and Gliogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sections were incubated with rat anti-BrdU antibody (1:200, Abcam, Cambridge, UK), which was detected using CY3-labeled anti-rat IgG antibody (1:200, Millipore) and mouse anti-neuronal nuclei (NeuN) antibody (1:500, Millipore), which was detected using fluorescein-labeled anti-mouse antibody (1:50, Millipore) or mouse anti-GFAP antibody (1:200, Millipore), which was detected using a FITC-labeled anti-mouse antibody (1:50, Millipore). BrdU⁺/NeuN⁺ or GFAP⁺ cells were observed using a confocal laser-scanning microscope (Leica, Heidelberg, Germany). The number of BrdU⁺/NeuN⁺ or GFAP⁺ cells per section (200 μm apart) was multiplied by 5 to obtain the total number.

Zhang T., Hong J., Di T, & Chen L. (2016). MPTP Impairs Dopamine D1 Receptor-Mediated Survival of Newborn Neurons in Ventral Hippocampus to Cause Depressive-Like Behaviors in Adult Mice. Frontiers in Molecular Neuroscience, 9, 101.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!