Cd45 pe

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

CD45-PE is a fluorescently-labeled antibody that binds to the CD45 antigen, a protein tyrosine phosphatase expressed on the surface of hematopoietic cells. It is used in flow cytometry applications to identify and analyze various cell types within a sample.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CD45-PE-Cy7 (52 citations) Anti-CD45-PE (24 citations) Anti-CD45-PE-Cy7 (12 citations) CD45.2-PE (10 citations) Anti-mouse CD45-PE-Cy7 (7 citations) CD45-PE (30-F11, (5 citations) PE anti-mouse CD45 (5 citations) Anti-CD45.1-PE (4 citations) CD45 PE-cyanine 7 (3 citations) Anti-CD45.2-PE (clone 104) monoclonal antibodies (3 citations)

59 protocols using cd45 pe

Flow Cytometry Analysis of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested and the cell pellet was resuspended in PBS to 10⁶ cells/100 μl. The cell suspension was incubated with the following antibodies: HLA-DR-APC, CD34-FITC, CD45-PE, CD73-PE, CD90-FITC, and CD105-FITC (eBioscience). An isotype control antibody (eBioscience) was used for each. Approximately 5 μl of each antibody (1:10 dilution, according to the antibody datasheets) per tube was added, and this was incubated for 15 min in the dark at 2–8 °C. Cells were completely washed with PBS and resuspended in 1% (w/v) paraformaldehyde (Sangon Biotech, China). Samples were subjected to flow cytometry (BD Biosciences, USA) and the data were analyzed by FlowJo software (FlowJo LLC, Ashland, OR, USA).

Li X.C., Wang M.S., Liu W., Zhong C.F., Deng G.B., Luo S.J, & Huang C.M. (2018). Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence. Stem Cell Research & Therapy, 9, 171.

+ Open protocol

+ Expand

Isolation and Flow Cytometry of Intestinal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following crypt isolation, the crypt suspensions were pelleted (100 g, 5 min, 4°C) and supernatant was discarded. Crypts were resuspended in TrypLE (GIBCO, no phenol red, 12604039) and dissociated into individual cells by warming crypts in water bath at 32°C for 60 s. Dissociated single cells were treated with the following antibody cocktail for flow cytometry analysis: CD45-PE (eBioscience, 12-0451-83), CD31-PE (Biolegend, 102514), Ter-119PE (Biolegend, 116208), CD24-Pacific Blue (Biolegend, 101820), CD117-APC/Cy7 (Biolegend, 105826), and EpCAM-APC (eBioscience, 17-5791-82). 7AAD (Invitrogen, A1310) was used a viability dye to exclude dead cells from the analysis. ISCs were isolated as Lrg5-eGFP^hiEpCAM⁺CD24^low/−CD31⁻Ter119⁻CD45⁻7AAD⁻. eGFP^low progenitors were isolated as Lrg5-eGFP^lowEpCAM⁺CD24^low/−CD31⁻Ter119⁻CD45⁻7AAD⁻. Cells were sorted using a BD FACS II SORP cell sorter.

Mana M.D., Hussey A.M., Tzouanas C.N., Imada S., Millan Y.B., Bahceci D., Saiz D.R., Webb A.T., Lewis C.A., Carmeliet P., Mihaylova M.M., Shalek A.K, & Yilmaz Ö.H. (2021). High-fat diet-activated fatty acid oxidation mediates intestinal stemness and tumorigenicity. Cell reports, 35(10), 109212.

+ Open protocol

+ Expand

Chondrocyte Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chondrocytes (1×10⁶⁾ from growth plate (passage 1) were washed in PBS and incubated with corresponding primary antibodies for one hour at 4°C. Primary antibodies used here were conjugated antibodies specific for CD44-FITC (dilution 1:100, #550974; BD Biosciences, San Diego, CA, USA), CD29-PE (dilution 1:80, #48-0291; eBiosciences; Thermo Fisher Scientific, Inc.), CD34-PE (dilution 1:100; #sc-74499 PE; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), CD45-PE (dilution 1:80, #12-0461; eBiosciences; Thermo Fisher Scientific, Inc.) and unconjugated antibodies for CD105 (dilution 1:100, #ab11414) and CD146 (dilution 1:80, #ab75769) (both from Abcam, Cambridge, MA, USA). After washing with PBS, cells stained with CD105 and CD146 were incubated with anti-mouse FITC-conjugated secondary antibody (dilution 1:500, #A0568; Beyotime Institute of Biotechnology, Guangzhou, China) or Alexa Fluor 594 donkey anti-rabbit IgG (dilution 1:500, #A-21207; Invitrogen; Life Technologies) for 30 min at 4°C. The cells were washed twice and then re-suspended in 200 µl PBS for the flow cytometry analysis (FACSort; BD Biosciences).

Wu Y.X., Jing X.Z., Sun Y., Ye Y.P., Guo J.C., Huang J.M., Xiang W., Zhang J.M, & Guo F.J. (2017). CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro. Molecular Medicine Reports, 16(6), 8019-8028.

+ Open protocol

+ Expand

Tumor Macrophage Profiling via Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

To prepare single-cell suspensions for flow cytometry, fresh tumor tissue was dissected into approximately 1 to 3 mm³ fragments and digested with 80 U/mL collagenase (Invitrogen) in DMEM containing 10% FBS for one hour at 37°C while shaking. After red blood cell (RBC) lysis, single-cell suspensions were filtered and incubated for 20 minutes on ice with the following antibodies (1:100): CD45-PE (eBioscience, San Diego, CA), F4/80-PE-Cy7 (eBioscience, San Diego, CA), CD11b-FITC (BD Biosciences San Jose, CA), CD206-APC (Biolegend, San Diego, CA),CD68-PerCP-Cy5.5 for macrophages (Biolegend, San Diego, CA), Cells were washed with phosphate-buffered saline (PBS) before analysis on the BD LSR-II flow cytometer (Beckman Coulter).

Liang P., Henning S.M., Guan J., Grogan T., Elashoff D., Cohen P, & Aronson W.J. (2019). Effect of Dietary Omega-3 Fatty Acids on Castrate Resistant Prostate Cancer and Tumor Associated Macrophages. Prostate cancer and prostatic diseases, 23(1), 127-135.

+ Open protocol

+ Expand

Inflammasome Activation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HDM was from Greer Laboratories. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042) and anti-NLRP3 (AG-20B-0014) antibodies were from Adipogen. Anti-ASC (67824) antibody was from Cell Signaling Technology. Anti-β-actin (66009-1-Ig) was from Proteintech.
Anti-mouse antibodies used for flow cytometry were: CD3-FITC (BD, 553062, 145-2C11), CD19-FITC (eBioscience, 11-0193-82, 1D3), Ly-6G-PE (Biolegend, 127608, 1A8), CD45-PE (eBioscience, 12-0451-81, 30-F11), CD11c-PerCP-Cy5.5 (Biolegend, 117328, N418), CD11b-PerCP-Cy5.5 (BD, 550993, M1/70), CD11b-PE-Cy7 (eBioscience, 25-0112-82, M1/70), CD3e-PE-Cy7 (BD, 552774, 145-2C11), CD19-PE-Cy7 (Biolegend, 115520, 6D5), SiglecF-Alexa Fluor 647 (BD, 562680, E50-2440), MHCII-APC-eFluor 780 (eBioscience, 47-5321-82, M5/114.15.2), Ly-6G-BV421 (Biolegend, 127628, 1A8), CD45-BV510 (Biolegend, 103137, 30-F11), CD11c-BV510 (Biolegend, 117338, N418). Ultrapure LPS was obtained from Invitrogen. Nigericin was obtained from Sigma-Aldrich. RRx-001 (S8405) was from Selleck.

Ma M., Li G., Qi M., Jiang W, & Zhou R. (2021). Inhibition of the Inflammasome Activity of NLRP3 Attenuates HDM-Induced Allergic Asthma. Frontiers in Immunology, 12, 718779.

+ Open protocol

+ Expand

Isolation of Murine Lung Cell Types

Check if the same lab product or an alternative is used in the 5 most similar protocols

Murine lung tissue was digested into a single-cell suspension as previously described using dispase (Corning catalog #354235), collagenase (Roche catalog #10103578001), and DNase (Roche catalog #10104159001). When isolating AT2 cells from Sftpc^CreERT2:R26R^TdTomato mice, the animals received 200μg/gm tamoxifen (Sigma-Aldrich catalog #T55648) in corn oil (Sigma-Aldrich catalog #C8267) via gavage 3 days prior to being euthanized. Tomato+ cells were isolated using flow cytometry (MoFlow Astrios with Summit v 6.3.0.16900 software). To isolate AT2 cells from mice we stained single-cell suspensions of murine lung tissue and gated based on the following criteria: positive for EpCAM-APC (BioLegend catalog #118213) and negative for CD31-PE (eBioscience catalog #12-0311-81), CD45-PE (eBioscience catalog #12-0451-81), podoplanin-PE (eBioscience catalog #12-5381-80), Sca1-PE (eBioscience catalog #12-5981-81), CD24-PE (BioLegend catalog #119307), and DAPI (BioLegend catalog #422801) as previously described^{81 (link)}. To isolate mesenchymal cells we sorted for cells that were positive for CD140a (BioLegend catalog #135907) and negative for and DAPI (BioLegend catalog #422801). Antibody concentrations are in Supplementary Table 2.

Paris A.J., Hayer K.E., Oved J.H., Avgousti D.C., Toulmin S.A., Zepp J.A., Zacharias W.J., Katzen J.B., Basil M.C., Kremp M.M., Slamowitz A.R., Jayachandran S., Sivakumar A., Dai N., Wang P., Frank D.B., Eisenlohr L.C., Cantu E I.I.I., Beers M.F., Weitzman M.D., Morrisey E.E, & Worthen G.S. (2020). STAT3-BDNF-TrkB signaling promotes alveolar epithelial regeneration after lung injury. Nature cell biology, 22(10), 1197-1210.

+ Open protocol

+ Expand

Expanded PPCs Immunophenotyping by Flow

Check if the same lab product or an alternative is used in the 5 most similar protocols

For flow cytometry analysis of PPCs, the cells were harvested, washed with PBS, resuspended in PBS, and stained with fluorescent antibodies for 60 min at 4 °C in the dark. We used CD73-PE (eBioscience, San Diego, CA, USA), CD90-PE (eBioscience), CD105-PE (eBioscience), CD34-FITC (eBioscience), CD45-PE (eBioscience), and HLA-DR-FITC (eBioscience) to characterize the expanding PPCs. Isotype-matched antibodies served as controls.

Zou C., Lu Y., Teng X., Wang S., Sun X., Huang F., Shu G., Huang X., Guo H., Chen Z., Zhang J, & Zhang Y.A. (2017). MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys. Scientific Reports, 7, 2505.

+ Open protocol

+ Expand

Monocyte phenotyping with cancer cell interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human monocytes were cultured alone or with mf or three different CMFDA-labeled cancer cell lines (green; FITC channel) as mentioned above. After 48 hrs, cells were harvested, washed with PBS and incubated with 10 μl human IgG (10 mg/ml; Sigma-Aldrich, St. Louis, MO) for 10 min at 4°C to inhibit nonspecific binding through FcγRs and then incubated with marker-specific mAb conjugated with PDL2- APC (eBioscience, Cat No. 17-5888-42), VCAM1(Vascular cell adhesion molecule-1)-APC (Biolegend, Cat No. 305810), CD14-APC Cy7 (eBioscience, Cat No. 47-0149-42), CD45-Pacific blue (Biolegend, Cat No. 304022), CD206 (Mannose receptor)-Percp efluor 710 (eBioscience, Cat No. 46-2069-42), PDL1-PE-Cy7 or PDL1-APC (eBioscience, Cat No. 25-5983-42 and Cat No. 17-5983-41 respectively), or CD163-PE (eBioscience, Cat No. 12-1639-42), CD45-PE (eBioscience, Cat No. 12-9459-42) at saturating concentrations for 30 min at 4°C and washed twice with FACS medium. Monocyte cell populations (CD45⁺/CMFDA^-) were then identified and gated to measure the expression of cell surface markers.

Narasimhan P.B., Akabas L., Tariq S., Huda N., Bennuru S., Sabzevari H., Hofmeister R., Nutman T.B, & Tolouei Semnani R. (2018). Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Neglected Tropical Diseases, 12(4), e0006404.

+ Open protocol

+ Expand

Leukemia Development in Xenograft Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Two cell doses (1 × 10⁴ or 1 × 10⁶) of REH-scramble or REH-E5 cells were transplanted into NOD/scid IL-2Rg(null) mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ; The Jackson Laboratory, Bar Harbor, ME, USA) by tail vein injection. Each recipient group had three mice. Leukemic development and survival of mice were monitored daily. The recipients were sacrificed and dissected upon onset of physical symptoms of leukemia, including hind limb paralysis, weight loss, or decreased activity. Bone marrow and spleen cells were harvested, and single-cell suspensions were prepared. REH cells were detected with flow cytometry, using CD45-PE and CD19-APC antibodies (eBiosciences, San Diego, CA; catalog no. 12-9459-42, 17-0199-42).

Ueno S., Lu J., He J., Li A., Zhang X., Ritz J., Silberstein L.E, & Chai L. (2014). Aberrant expression of SALL4 in acute B cell lymphoblastic leukemia: Mechanism, function, and implication for a potential novel therapeutic target. Experimental hematology, 42(4), 307-316.e8.

+ Open protocol

+ Expand

Differential Immunophenotyping of NK Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used. From eBioscience (San Diego, CA): CD3-APC eFluor 780 (clone SK7); CD16-FITC (eBioCB16); CD19-APC eFluor 780 (HIB19); CD45-PE (HI30); CD94-FITC (DX22); Eomes-PE eFluor 610 (WD1928); Granzyme K-PerCP eFluor 710 (G3H69); HLA-A3-FITC (GAP.A3); IFN gamma-Alexa Fluor 488 (4S.B3); S1PR1-eFluor 660 (SW4GYPP); T-bet-PECy7 (4B10); TNF alpha-APC (Mab11). From Biolegend (London, UK): CCR5-APC (J418F1); CD49a-FITC (TS2/7); CD69-APC (FN50); CD103-FITC (Ber-ACT8); CX3CR1-FITC (2A9-1); CXCR6-PerCP Cy5.5 (K041E5); CXCR6-APC (K041E5); GM-CSF-PE (BDV-21C11); Granzyme B-FITC (GB11); HLA-A2-FITC (BB7.2); KIR2DL1/S1/S3/S5-APC (HP-MA4); KIR2DL2/L3-APC (DX27); KIR3DL1-APC (DX9); Perforin-APC (dG9). From BD (Oxford, UK): CD56-BV510 (NCAM16.2), LIF-PE (1F10). Dead cells were excluded using Fixable Viability Dye eFluor 450 (eBioscience). Intracellular staining was carried out using Human FoxP3 Buffer (BD) according to the manufacturer’s instructions. Data were acquired on a Fortessa II (BD) and analysed using FlowJo (Treestar, Ashland, OR). Cells were sorted on an Aria (BD). Eomes^lo NK cells were isolated by sorting on live cells (propidium iodide negative, Tonbo Biosciences, San Diego, CA), singlets, scatter, CD3^- CD56⁺ CXCR6^- CD16⁺. Eomes^hi NK cells were isolated by sorting on live cells, singlets, scatter, CD3^- CD56⁺ CXCR6⁺.

Cuff A.O., Robertson F.P., Stegmann K.A., Pallett L.J., Maini M.K., Davidson B.R, & Male V. (2016). Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. The Journal of Immunology Author Choice, 197(11), 4283-4291.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!