Cell Counting Kit-8 was obtained from Beyotime (Shanghai, China). Catechin (C), epicatechin (EC), epicatechin gallate (ECG), procyanidin B1 (B1), procyanidin B2 (B2), procyanidin B3 (B3), procyanidin B4 (B4), procyanidin B1-3-O-gallate (B1-G), procyanidin B2-3-O-gallate (B2-G), and procyanidin C1 (C1) were obtained from Caoyuankang Biotechnology. 2′,7′-Dichlorofluorescin diacetate, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) diagnostic kits were obtained from Solarbio (Beijing, China). Nuclear factor-erythroid 2-related factor 2-siRNA (Nrf2-siRNA), control-siRNA, and Lipofectamine 2000 were obtained from GenePharma (Shanghai, China). The primary antibodies, Nrf2, heme oxygenase 1 (HO-1), NAD(P)H: quinone oxidoreductase 1 (NQO1), Lamin B, and GAPDH, as well as corresponding secondary antibodies, were obtained from Proteintech (Wuhan, China). RNAiso Plus, PrimeScript RT reagent Kit with gDNA Eraser, and SYBR Premix Ex Taq II were purchased from Takara (Takara, Shiga, Japan).
2 7 dichlorofluorescin diacetate
Manufactured by Solarbio
Sourced in China
2',7'-Dichlorofluorescin diacetate is a non-fluorescent compound that can be oxidized to produce the highly fluorescent 2',7'-dichlorofluorescein. This reaction is commonly used to detect the presence of reactive oxygen species in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Dspe tk peg2000, by Xi’an Ruixi (3 mentions)
Sybr premix ex taq 2, by Takara Bio (2 mentions)
Rnaiso plus, by Takara Bio (2 mentions)
Primescript rt reagent kit with gdna eraser, by Takara Bio (2 mentions)
Gapdh, by Proteintech (2 mentions)
Lipofectamine 2000, by GenePharma (2 mentions)
Cck 8, by Beyotime (2 mentions)
Superoxide dismutase sod, by Solarbio (1 mentions)
Cell counting kit 8 (cck8), by Beyotime (1 mentions)
Ethyl 3 aminobenzoate methanesulfonate, by Merck Group (1 mentions)
Cholesteryl bodipy fl c12, by Thermo Fisher Scientific (1 mentions)
Cleaved caspase 3, by Proteintech (1 mentions)
Ve cadherin, by Proteintech (1 mentions)
β actin, by Proteintech (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
Bcl 2, by Proteintech (1 mentions)
Mitosox red, by MedChemExpress (1 mentions)
Nrf2 sirna, by Thermo Fisher Scientific (1 mentions)
Control sirna, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
6 protocols using 2 7 dichlorofluorescin diacetate
1
Antioxidant Assays and Gene Regulation
2
Lipid Fluorescence Imaging Assay
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Nile red and cholesterol were purchased from Sigma-Aldrich (St. Louis, MO, United States). Fluorescent cholesteryl (CholEsteryl BODIPY™ FL C12) was purchased from Thermo Fisher Scientific (Waltham, Massachusetts, U.S.). 2′,7′-Dichlorofluorescin diacetate was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Triglyceride (TG) kit, total cholesterol (TC) kit, superoxide dismutase (SOD) kit, and malondialdehyde (MDA) kit were purchased from Nanjing Jiancheng Biological Institute (Nanjing, China). Ethyl 3-aminobenzoate methanesulfonate was purchased from Sigma-Aldrich (St. Louis, MO, United States).
3
Emodin-Loaded Nanoparticles for Cellular Responses
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Emodin was obtained from Chenguang Biotechnology Co., LTD (Xi’an, China). Geniposide was purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). L-alpha-phosphatidyl choline (SPC) and cholesterol were acquired from Shanghai Ryon Biological Technology Co. Ltd (Shanghai, China). DSPE-TK-PEG2000 was obtained from Xi’an RuiXi Biological Technology Co., LTD (Xi’an, China). Additionally, 2′,7′-Dichlorofluorescin diacetate was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China), MitoSox Red was obtained from MCE (MedChemExpress), and MitoQ was acquired from GLPBIO. Antibodies, including VE-cadherin, cleaved caspase-3, BAX, Bcl-2, and β-actin, were purchased from Proteintech (IL, USA).
4
Phytochemicals in Neuroprotection
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Catechin (C), epicatechin (EC), epicatechin gallate (ECG), procyanidin B1 (B1), procyanidin B2 (B2), procyanidin B3 (B3), procyanidin B4 (B4), procyanidin B1-3-O-gallate (B1-G), procyanidin B2-3-O-gallate (B2-G), and procyanidin C1 (C1) were obtained from Caoyuankang Biotechnology (Caoyuankang, Cheng Du, China). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) 1-methyl-4-phenylpyridinium (MPP+) agents were purchased from Sigma (Sigma, St. Louis, MO, USA), and CCK-8 was purchased from Beyotime (Beyotime, Shanghai, China). 2′,7′-Dichlorofluorescin diacetate, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) diagnostic kits were purchased from Solarbio (Solarbio, Beijing, China). The cytotoxicity detection kit was purchased from Nanjing Jiancheng Institute of Bioengineering (Nanjing Jiancheng, Nanjing, China). Nrf2-siRNA, control-siRNA, and lipofectamine 2000 were obtained from Invitrogen (Invitrogen, Carlsbad, CA, USA). The following primary antibodies and corresponding secondary antibodies were obtained from Proteintech: Nrf2, HO-1, NAD(P)H: NQO1, B-cell lymphoma-2 (Bcl-2), Bax, lamin B, and GAPDH (Proteintech, Wuhan, China).
5
Antioxidant Pathway Activation Assay
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H2O2 was purchased from HUSHI (HUSHI, Shanghai, China). CCK-8, NAC were purchased from Beyotime (Beyotime, Shanghai, China). PCs, 2′,7′-dichlorofluorescin diacetate, MDA, GSH-Px, SOD diagnostic kits, and CAT diagnostic kits were purchased from Solarbio (Solarbio, Beijing, China). RNAiso Plus, PrimeScript RT reagent Kit with gDNA Eraser, and SYBR Premix Ex Taq II were purchased from Takara (Takara, Shiga, Japan). Nrf2-siRNA, control-siRNA, and lipofectamine 2000 were obtained from GenePharma (GenePharma, Shanghai, China). The following primary antibodies and corresponding secondary antibodies were obtained from Proteintech (Proteintech, Wuhan, China): Nrf2, HO-1, GCLC, GCLM, NAD(P)H: NQO1, lamin B, and GAPDH.
6
Intracellular ROS Measurement by FACS
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Intracellular ROS was analyzed using FACS Calibur flow cytometer (BD Bioscience) in conjunction Cell-Quest Pro software. Briefly, treated cells were seeded into six-well culture dishes (1 × 10 5 cells/well) and grown overnight. After being washed three times with PBS, cells were incubated with 20 μM of 2', 7'-dichlorofluorescin diacetate (Beijing Solarbio Science & Technology Co., Ltd) for 20 min at 37°C. The fluorescence intensity was acquired with BD FACS calibur (BD Biosciences) and the amount of ROS was calculated using Cell Quest Pro software.
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