For tissue distribution of mRNA and protein expressions, data on the target genes were obtained from “The Tissue Atlas” category of “The Human Protein Atlas/HPA” (http://www.proteinatlas.org/ ) [51 (link)]. The mRNA expression from the “The Genotype-Tissue Expression/GTEx Dataset” was chosen for demonstration with reference to the normalized consensus dataset Immunohistochemistry staining was performed on normal human tissue samples. AR expression was detected with a rabbit antibody (1:250, HPA004733, Sigma-Aldrich) and validated with another rabbit antibody (1:500, GTX62599, GeneTex). ACE2 expression was primarily detected with a rabbit antibody (1:250, HPA000288, Sigma-Aldrich) and confirmed with a mouse antibody (1:5000, CAB026174, R&D Systems); TMPRSS2 expression was detected with a rabbit antibody (1:300, HPA035787, Sigma-Aldrich). The information on expression intensity and specific cell types that express respective genes were extracted from the staining reports for each staining type in the database.
Gtx62599
Manufactured by GeneTex
GTX62599 is a qPCR instrument designed for real-time quantitative PCR analysis. It features high-performance optics, precise temperature control, and advanced data analysis software to deliver accurate and reliable results for gene expression studies and other molecular biology applications.
Automatically generated - may contain errors
2 protocols using gtx62599
1
Tissue Expression Profiling of AR, ACE2, and TMPRSS2
2
Immunohistochemical Analysis of Androgen Receptor
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Rat penile specimens were formalin fixed, paraffin embedded and serially sectioned at 7 µm. Immunohistochemistry was carried out as previously described (Rodriguez et al., 2012b) utilizing anti-androgen receptor (rabbit monoclonal, diluted 1:200, GTX 62599, Genetex, Irving, CA). Signal detection was achieved using the Vector ABC System (Vector Laboratories, Foster City, CA, USA) followed by exposure to diaminobenzidine (Sigma®). Sections exposed to all steps except the application of the primary antibodies were used as negative controls.
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