Triton wr 1339

Mice were bled to measure baseline plasma triglyceride concentration under fasting conditions. Then, anesthetized mice were injected intravenously with Triton WR-1339 (Sigma-Aldrich, St. Louis, MO) at a dose of 500 mg/kg dissolved in a 15% solution of 0.9% NaCl, which inhibited lipolysis completely [23 (link)]. Blood was collected after the Triton injection and plasma triglycerides were measured. Triglyceride accumulation in the serums 60 and 120 min after injection was compared between mice of each genotype.

TG production was measured in 3-h fasted mice after intravenous administration of Triton WR-1339 (500 mg/kg, Sigma, Saint Louis, MO, USA), an inhibitor of lipolysis. Postprandial TG clearance was measured after 10 weeks of ad libitum access to 20% fructose supplementation to drinking water or with transgenic expression of Cholesteryl Ester Transfer Protein (CETP), a genetic model of impaired TG clearance [44 (link)]. Transgenic CETP mice (C57BL/6-Tg(CETP)UCTP20Pnu/J, Strain: 001929, Jackson Laboratories), were bred with Δhep mice to generate fl/fl (CETP⁺/SHP^fl/fl) and Δhep (CETP⁺/SHP^Δhep) mice. TG clearance was measured in 12-h fasted mice after oral gavage of olive oil (200 μL/mouse) with serial tail blood samples over 7–8 h.

Ultra-pure water (<5 µg/L TOC, (total organic carbon)) was obtained from a water purification system Arium 126 61316-RO, plus an Arium 611 UV unit (Sartorius, Goettingen, Germany). Methanol (HPLC grade) and formic acid (puriss. p.a. for mass spectrometry) from J. T. Baker (Phillipsburg, NJ, USA) were obtained. Dichloromethane (HPLC grade) were from Merck (Santiago, Chile). Commercial Folin–Ciocalteu (FC) reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric chloride hexahydrate, 2,4,6-tris(2-pyridyl)-s-triazine, trolox, quercetin, gallic acid, atorvastatin, Triton WR-1339 and DMSO were purchased from Sigma-Aldrich Chem. Co. (St Louis, MO, USA).

Triton WR 1339 with CAS no. 25301-02-4 and molecular formula and weight C17H28O3 and 280.40200, respectively, was purchased from Sigma-Aldrich (Sigma-Aldrich Chemie GmbH, Export Department, Eschenstrasse5, 82024 Taufkirchen, Germany).

Mice were fasted for 4 h and injected with vehicle or Triton WR 1339 (Sigma) at a dose of 500 mg/kg BW intravenously (62 (link)). Blood samples were collected at 0, 1.5, 3, and 4 h after injection. Plasma TG levels were measured by the Triglyceride assay kit (Pointe Scientific).

The following reagents were purchased from Sigma Chemical Co. (Taufkirchen, Germany): Folin-Ciocalteu, Gallic Acid, Ascorbic Acid, Quercetin, DPPH (1,1-diphenyl-2- picrylhydrazyl), Sodium Hydroxide (NaOH), Sodium Nitrate (NaNO₃), Aluminum Chloride (AlCl₃), β-carotene, Butylated Hydroxyanisole (BHA), Linoleic Acid, Tween-80, Potassium Ferricyanide [K₃Fe(CN)₆], Trichloroacetic Acid (TCA), n-Butanol, Methanol, Ethanol, Chloroform, Cholesterol, Deoxycholic Acid, Sodium Phosphate (Na₃PO4), Sodium Phosphate dibasic (Na₂HPO₄), Sodium Phosphate monobasic (NaH₂PO₄), Sodium Carbonate (Na₂CO₃), Ferric Chloride (FeCl₃), Triton WR-1339, Thiobarbituric Acid (TBA), and Copper Sulfate (CuSO₄).