Cells were cultivated in a 6-well cell culture plate (106 cells/well) and were then transfected with 1ng lncRNA-SNHG7 plasmid (GenePharma Co., Ltd., Shanghai, China), 1ng control plasmid, 100 nM miR-449a mimics (Guangzhou Ribobio Co., Ltd, Guangzhou, China), 100 nM mimics control (Guangzhou Ribobio Co., Ltd), 50 nM miR-449a inhibitor (Guangzhou Ribobio Co., Ltd), 50 nM control inhibitor (Guangzhou Ribobio Co., Ltd), 0.2 µM control-small interfering RNA (control-siRNA; Santa Cruz Biotechnology, Dallas, TX, USA), or 0.2 µM si-TNIP2 (Santa Cruz Biotechnology, Dallas, TX, USA) using Lipofectamine® 2000 (Life Technologies Corporation, Savant, MA, USA), according to the manufacturer’s instructions.
Mir 449a inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-449a inhibitor is a laboratory reagent designed to inhibit the expression of the MiR-449a microRNA. It functions by binding to and blocking the activity of the MiR-449a microRNA, which is involved in various cellular processes. The MiR-449a inhibitor is used in research applications to study the role of MiR-449a in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Mir 449a mimic, by RiboBio (3 mentions)
Inhibitor control, by RiboBio (1 mentions)
Mimics control, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax kit, by Thermo Fisher Scientific (1 mentions)
Hec 1a, by Procell (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Si nc, by RiboBio (1 mentions)
Oe nc, by Genechem (1 mentions)
3 protocols using mir 449a inhibitor
1
Transfection of lncRNA-SNHG7 and miR-449a in Cells
2
Endometrial-stromal and EC cell line culture
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We procured the human endometrial-stromal cell line (hESC) from Otwo Biotech, Ltd (Shenzhen, China) and the human EC cell lines (KLE, Ishikawa and HEC-1A) from Procell Life Science & Technology, Ltd (Wuhan, China). The cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) at 37°C with 5% CO2. The shRNA-negative control (sh-NC) and shRNA-LINC01106-1/-2 (sh-LINC01106-1/-2) were procured from Sangon Biotech, Inc (Shanghai, China). Overexpression-MET (pcDNA-MET) and its negative control (pcDNA-NC), miR-449a mimics, miR-449a inhibitor and their negative control (miR-NC) were all obtained from Ribo Biotech, Ltd (Guangzhou, China). The above agents were transfected into the cells using a Lipofectamine RNAiMAX kit (Invitrogen, Carlsbad, CA, USA). After treatment for 48 h, the cells were harvested to perform the subsequent trails.
3
siRNA and miR-449a Regulation of Circular RNAs
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Circ-100290 specialized small interfering RNAs (siRNAs), miR-449a inhibitor, and miR-449a mimic, along with their respective si-NC, miR-449a inhibitor-NC, and miR-449a mimic-NC, were purchased from RiboBio (Guangzhou, China). Plasmids for overexpressing circ-100290 (oe-circ) and its respective negative control (oe-NC) were purchased from Genechem (Shanghai, China). Transfections were performed with Lipofectamine 2000 transfection reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's protocol. After 48-h transfection, circ-ABCB10, eNOS, and VEGFA levels were assessed by qPCR. Sequences of qPCR primers are shown in Table S1 .
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