Hc pl apo cs 63 1.30 glycerine objective

Ten-day-old seedlings of the wild type, bzip19/23 mutant, and bzip19/23-OEOs48, bzip19/23-OEOs49, and bzip19/23-OEOs50 lines were grown in control or –Zn MS medium. For laser scanning confocal microscopy (LSCM) analysis, roots were transferred to microscope slides containing propidium iodide (PI) to stain root cell walls, and were visualized using a Leica TCS SP5 II laser scanning confocal inverted microscope (Leica Microsystems) with a HC PL APO CS ×63/1.30 Glycerine objective. Argon 458 nm and 514 nm laser lines were used for CFP and PI excitation, respectively. The emission settings were between 470 nm and 500 nm for CFP and 590 nm and 630 nm for PI. For each complementation line, three independently transformed T₃ homozygous lines were tested, with observations of 2–3 seedlings per line and Zn condition.

Fourteen-day-old seedlings of the bzip19/23-OEMtFbZIP1 and bzip19/23-pZIP4:GUS-OEMtFbZIP2 lines grown in control or –Zn MS medium were analyzed with confocal laser scanning microscopy (CLSM). Roots were incubated in 50 μM propidium iodide (PI) solution for 30 s to stain cell walls. Roots were visualized using a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) with a HC PL APO CS × 63/1.30 Glycerine objective. Argon 458 and 514 nm laser lines were used for CFP and PI excitation, respectively. The emission settings were between 470 and 533 nm for CFP and between 602 and 684 nm for PI. Three independently transformed lines were analyzed, with observations of 2–3 seedlings per line and Zn treatment.