BAY‐876 and BMS‐1 were purchased from MedChemExpress (MCE, China). L‐Ala‐NCA (CAS: 2224‐52‐4) and mPEG45‐NH2 (CAS: 80506‐64‐5) were purchased from Aladdin (China). LA assay kits were obtained by Jianchengbio (China). CCK‐8 assays, lysed RIPA buffer, PMSF, DAPI anti‐β‐tubulin antibody, antiIgG‐FITC antibody, anti‐IgG‐Cy3 antibody, and anti‐GLUT1 antibody, anti‐PD‐L1 antibody, HRP‐conjugated anti‐rabbit IgG and antimouse IgG were purchased from Servicebio (China). Murine IFN‐γ, TNF‐α, and TGF‐β ELISA kits, a cytokine of murine IFN‐γ were provided by Neobioscience (Shenzhen, China). anti‐PD‐L1‐APC antibody, anti‐CD80‐PE antibody, anti‐MHCII‐FITC antibody, anti‐CD45‐PE antibody, anti‐CD45‐FITC antibody, anti‐CD11b‐PerCP‐Cy5.5 antibody, anti‐CD3‐PerCP‐Cy5.5 antibody, anti‐CD206‐APC antibody, anti‐CD4‐FITC antibody, anti‐Foxp3‐APC antibody, anti‐CD11b‐PerCP‐Cy5.5 antibody, anti‐IFN‐γ‐APC antibody were purchased from Liankebio (China). anti‐CD206‐APC antibody was provided by Elabscience (China).
Anti pd l1 antibody
Manufactured by Wuhan Servicebio Technology
Sourced in China, United States
The Anti-PD-L1 antibody is a laboratory product used in research applications. It binds to the PD-L1 protein, which is involved in regulating the immune system. The antibody can be utilized in various immunological and cell-based assays.
Automatically generated - may contain errors
2 protocols using anti pd l1 antibody
1
Comprehensive Protocol for Tumor Immunotherapy
2
Immunohistochemical Analysis of POLD1, POLD3, and PD-L1 in Hepatocellular Carcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue microarrays containing 10 HCC samples and 10 adjacent normal liver samples with associated clinicopathological information were purchased from Shanghai Outdo Biotech Co. Ltd. (Cat# HLivH020PG02-M-255, China). Immunohistochemical staining (IHC) assays were performed on a tissue microarray using anti-POLD1 antibody (Cat#383045, dilution 1:200, ZEN-BIO, Chengdu, China), anti-POLD3 antibody (Cat#bs-2755R, dilution 1:200, Bioss, Boston, USA) and anti-PDL1 antibody (Cat#GB14132, dilution 1:200, Servicebio, Wuhan, China). The IHC staining score (H-score) can be divided into two parts: staining intensity and percentage of positive tumor cells. Staining intensity was classified as 0 (no staining), 1 (weak staining), 2 (moderate staining), and 3 (strong staining). The percentage of positive tumor cells was classified as 1+ (≤25%), 2+ (26–50%), 3+ (51–75%), and 4+ (>75%). The final expression scores were calculated by multiplying the two variables together.
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