Bis acrylamide

Manufactured by Avantor

Sourced in United States

Bis-acrylamide is a chemical compound commonly used in the production of polyacrylamide gels for electrophoresis applications in molecular biology and biochemistry. It serves as a cross-linking agent, helping to create a stable and uniform gel matrix for the separation and analysis of proteins, nucleic acids, and other macromolecules.

Automatically generated - may contain errors

Lab products found in correlation

Acrylamide, by Avantor (4 mentions) Ammonium persulfate, by Merck Group (3 mentions) Glycine, by Avantor (2 mentions) Methanol, by Avantor (2 mentions) Sodium dodecyl sulfate (sds), by Avantor (2 mentions) Tween 20, by Avantor (2 mentions) Tris base, by Avantor (2 mentions) Ethylene diamine tetraacetic acid (edta), by Avantor (2 mentions) Ponceau s, by Merck Group (2 mentions) Temed, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Folic acid, by Merck Group (1 mentions) Bromophenol blue, by Bio-Rad (1 mentions) Phenylmethylsulfonyl fluoride, by Avantor (1 mentions) Tris hcl, by Avantor (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Nessler s reagent, by Merck Group (1 mentions) Deae sepharose fast flow media, by GE Healthcare (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions)

6 protocols using bis acrylamide

Western Blot Reagent Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ponceau S, Ammonium Persulfate and TEMED were products of Millipore-Sigma Canada. Tween20, Tris-base, Glycine, EDTA, Methanol, SDS, Acrylamide and Bis-Acrylamide were obtained from VWR, Canada. All chemicals were of ACS grade or higher.

Feng D., Gao P., Henley N., Dubuissez M., Chen N., Laurin L.P., Royal V., Pichette V, & Gerarduzzi C. (2022). SMOC2 promotes an epithelial-mesenchymal transition and a pro-metastatic phenotype in epithelial cells of renal cell carcinoma origin. Cell Death & Disease, 13(7), 639.

+ Open protocol

+ Expand

Gel Electrophoresis Protocol for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Folic Acid (FA), Ponceau S, Ammonium Persulfate and TEMED were products of Sigma-Aldrich CAN. Tween20, Tris-base, Glycine, EDTA, Methanol, SDS, Acrylamide and Bis-Acrylamide were obtained from VWR. All chemicals were of ACS grade or higher. Trimidox and Torbugesic were obtained from CDMV, Saint-Hyacinthe, CAN.

Feng D., Ngov C., Henley N., Boufaied N, & Gerarduzzi C. (2019). Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury. Scientific Reports, 9, 16736.

+ Open protocol

+ Expand

Purification and Characterization of L-Asparaginase

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture media components such as tryptone extract and Bacto yeast extract were purchased from Difco Laboratories, India. Phenylmethylsulfonyl fluoride (PMSF), isopropyl β-D-1-thiogalactopyranoside (IPTG), acrylamide, bis-acrylamide, Tris–HCl, and L-asparagine were from Amresco, United States. Sodium dodecyl sulfate (SDS), ammonium persulfate (APS), dithiothreitol (DTT), urea, Nessler’s reagent, and trichloroacetic acid (TCA) were from Sigma-Aldrich, United States. 2,2,2-Trifluoroethanol was from SRL, India. Tetramethyl ethylenediamine (TEMED), ethylenediaminetetraacetic acid (EDTA), and bromophenol blue were from Bio-Rad, United States. Coomassie Brilliant Blue R-250 and ampicillin were from USB Corporation, United States. Glacial acetic acid and methanol were from Merck’s EMPARTA^®. Ethanol, n-propanol, and glycerol were of analytical grade and were from Spectrochem, India. DEAE-Sepharose Fast Flow media was purchased from GE Healthcare, United Kingdom. BCA assay kit, SDS-PAGE prestained molecular weight marker, and commercial r-hGH were from Thermo Fisher Scientific, United States. Commercial L-asparaginase 5,000 IU/ml, Bionase^® 5K, was purchased from Zydus Cadila, India. RPMI, horse serum (HS), and fetal bovine serum (FBS) were from Gibco BRL, United States. All the other chemicals were of analytical grade.

Singhvi P., Verma J., Panwar N., Wani T.Q., Singh A., Qudratullah M., Chakraborty A., Saneja A., Sarkar D.P, & Panda A.K. (2021). Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method. Frontiers in Microbiology, 12, 618559.

+ Open protocol

+ Expand

Protein Modification and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acrylamide (AppliChem GmbH, Darmstadt, Germany); bis-Acrylamide (Amresco, Solon, OH, USA); HSA (fraction V; Renal, Budapest, Hungary); Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one (C₂₀H₁₈N₂O₂)) (Merck, Darmstadt, Germany); 1 M MgCl₂ (M1028-1ML) (Sigma, St. Louis, MO, USA); NaCl, (NH₄)₂S₂O₈ (Acros Organics, Waltham, MA, USA); N,N,N′,N′-tetramethyl ethylenediamine (TEMED) (Bio-Rad Laboratories, Berkeley, CA, USA); Tris (Fisher Scientific, Pittsburgh, PA, USA); AcOH (ice-cold) and Stains-All (Acros Organics, Waltham, MA, USA); xylene cyanol (Sigma, St. Louis, MO, USA); Ficoll 400 (Pharmacia, Stockholm, Sweden); DMEM; DMEM F12; Fetal Bovine Serum (FBS); GlutaMAX Supplement; Antibiotic-Antimycotic; and TrypLE (Gibco, Waltham, MA, USA) were used in this work. The isolation and purification of the HSA monomeric fraction were performed according to [68 (link)]. HSA modified by sulfo-Cy5 dye (HSA-Cy5) was obtained according to [69 (link)]. For the solutions, Milli-Q (18.2 M × Om/cm) water (purified by Simplicity 185 water system (Millipore, St. Louis, MO, USA)) was used.

Bauer I., Ilina E., Zharkov T., Grigorieva E., Chinak O., Kupryushkin M., Golyshev V., Mitin D., Chubarov A., Khodyreva S, & Dmitrienko E. (2023). Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 15(12), 2779.

+ Open protocol

+ Expand

Polyacrylamide Gel Electrophoresis Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acrylamide (40% w/v) and Bis(Acrylamide) (2% w/v) solutions were bought from Amresco and used without further purification. Tetramethylethylenediamine (TEMED), ammonium persulfate (APS), and sodium acrylate were purchased from Sigma-Aldrich and used as received. Sodium polystyrenesulfonate (NaPSS) of five molecular weights (126, 234, 587, 1188, 2270 kDa) was purchased from ScientiÞc Polymers (catalog numbers 624, 625, 626, 628, and 923). The solutions were prepared using deionized water and sodium chloride. The Hydrophilic Polyvinylidene Fluoride (PVDF) filters with two pore sizes 220 and 450 nm were purchased from Millex Company.

Jia D, & Muthukumar M. (2018). Topologically frustrated dynamics of crowded charged macromolecules in charged hydrogels. Nature Communications, 9, 2248.

+ Open protocol

+ Expand

Synthesis of Tunable Polyacrylamide Hydrogels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acrylamide (Amresco, OH, USA) was dissolved in DI water to achieve a 40% w/v solution. Bis-Acrylamide (Amresco, OH, USA) was dissolved in DI water to achieve a 2% w/v solution. The two solutions were combined with DI water at different ratios to achieve PAM that would crosslink to different stiffnesses as described by Tse and Engler.^{53 (link)} To crosslink 1 mL of PAM, 10 μL of 0.438 M aqueous APS and 1 μL of TEMED were added, and the mixture was either pipetted up and down multiple times or sonicated for a few seconds. The PAM solution was left to crosslink for at least half an hour. PAM gels of 3 kPa, 4 kPa, 7 kPa, 11 kPa, 20 kPa and 40 kPa Young’s moduli were synthesized.

Bandara S.R., Molley T.G., Kim H., Bharath P.A., Kilian K.A, & Leal C. (2019). The structural fate of lipid nanoparticles in the extracellular matrix. Materials horizons, 7(1), 125-134.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!