Sk mes 1

NCI-H520 and SK-MES-1 were purchased from Cellcook Co., Ltd. (Guangzhou, China) with cell authentication via the STR multi-amplification method. NCI-H520 was cultured in RPMI1640 (Gibco BRL). SK-MES-1 was cultured in Eagle’s Minimum Essential Medium. All medium contained 10% FBS and 1% penicillin/streptomycin. The cells were cultured at 37°, under 5% CO₂. BSO (yuanye, Shanghai, China) was dissolved in H₂O. BSO was diluted to 249.7uM, followed by replacing the cell medium 12h after cells seeded. All experiments were completed less than 2 months after establishing stable cell lines or thawing early passage cells.

NCI-H520 and SK-MES-1 were purchased from Cellcook Co., Ltd. with cell authentication via STR multi-amplification method. KLN205 was obtained from Chinese Academy of Medical Sciences tumor cell libraries. NCI-H520 was cultured in RPMI1640 (Gibco BRL). SK-MES-1 was cultured in MEM. KLN205 was cultured in H-DMEM. All medium contained 10% FBS and 1% penicillin/streptomycin. For 12/15-LOX inhibitor treatment experiments, ML355 and PD146176 was diluted in medium, followed by replacing the cell medium 5 h after cells seeded respectively. Cell lines were routinely evaluated for Mycoplasma contamination. All experiments were completed less than 2 months after establishing stable cell lines or thawing early-passage cells.