Rabbit anti atg12

Manufactured by Cell Signaling Technology

Rabbit anti-Atg12 is a primary antibody that recognizes the Atg12 protein. Atg12 is an essential component of the autophagy pathway and plays a role in the formation of autophagosomes. This antibody can be used to detect and study the Atg12 protein in various experimental applications.

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Atg12 (64 citations) Anti-ATG12 (23 citations)

3 protocols using rabbit anti atg12

Autophagy Marker Immunoblotting Protocol

Cited 1 time

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The specific method is as mentioned before [28 (link)]. In this study, primary antibodies included rabbit anti-LC3B (1:1000; Sigma-Aldrich L7543), rabbit anti-P62 (1:1000; Abcam ab205719), mouse anti-β-Actin (1:1000; Sigma-Aldrich A5316), rabbit anti-Sidt2 (1:1000; Invitrogen PA5-69064), rabbit anti-Atg5 (1:1000; Cell Signaling Technology 9980S), rabbit anti-Atg7 (1:1000; Cell Signaling Technology 8558S), rabbit anti-Atg12 (1:1000; Cell Signaling Technology 2011S), mouse anti-LAMP1 (1:1000; Santa Cruz Biotechnology H4A3), rabbit anti-cathepsin B (1:1000; Cell Signaling Technology 31718S).

Geng M.Y., Wang L., Song Y.Y., Gu J., Hu X., Yuan C., Yang M., Pei W.J., Zhang Y, & Gao J.L. (2021). Sidt2 is a key protein in the autophagy-lysosomal degradation pathway and is essential for the maintenance of kidney structure and filtration function. Cell Death & Disease, 13(1), 7.

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Comprehensive Immunodetection Protocol for Autophagy

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Antibodies include: rabbit anti-actin (Sigma; ½,000), rabbit
anti-ATG12 (Cell
Signalling; 2010; 1/100), goat anti-CALM (Santa Cruz Biotechnology; sc-6433; 1/500), rabbit anti
PICALM (Sigma; 1/1,000),
rabbit anti-LC3 for western blotting (Novus Biologicals; NB100-2220;
¼,000), rabbit anti LC3 (Cell Signaling; 2775; 1/1,000), mouse
monoclonal anti-LC3 for immunofluorescence (Nanotools; clone 5F10; 1/200), mouse
anti-GFP (Clontech; 632569; 1/1,000), mouse anti-p62 (BD Transduction Lab; 610833;
1/1,000), rabbit anti-p62
(Novus Biologicals; NBP1-49954; 1/1,000), goat polyclonal DyLight 680
anti-rabbit IgG (Licor; 1/4,000) mouse anti-HA (Covance; clone 16B12; 1/1,000),
mouse anti-VAMP2 (SySy, clone
11C3; 1/500), rabbit anti-VAMP8 (SySy; 104302; 1/1,000), mouse anti-GAPDH (Abcam;
ab8245; ½,000), rabbit anti-ATG9A (Abcam; 2975-1; 1/200) and mouse anti-phosphorylated
tau (PHF1, Thermo;
MN1020; 1/200).
Reagents include: Baf A1
(Sigma), cholera toxin subunit
B conjugated to Alexa555 (Invitrogen), transferrin conjugated to Alexa647
(Invitrogen) and EGF
conjugated to Alexa555 (Invitrogen).

Moreau K., Fleming A., Imarisio S., Lopez Ramirez A., Mercer J.L., Jimenez-Sanchez M., Bento C.F., Puri C., Zavodszky E., Siddiqi F., Lavau C.P., Betton M., O’Kane C.J., Wechsler D.S, & Rubinsztein D.C. (2014). PICALM modulates autophagy activity and tau accumulation. Nature Communications, 5, 4998.

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Assessing Apoptosis and Autophagy Markers

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All the chemicals used in this study were dissolved in dimethyl sulfoxide (DMSO). Bafilomycin A1 was from Millipore; MG132; staurosporine and embelin were from Sigma. Primary antibodies used were: rabbit anti-XIAP, rabbit anti-Beclin 1, rabbit anti-Atg12 and anti-P-IκB Ser32/Ser36 (all diluted at 1:1000, Cell signaling), rabbit anti-actin and mouse anti-tubulin (both diluted at 1:4000, Sigma), rabbit anti-LC3 (diluted at 1:1000, Novus Biological), mouse anti-p62 (diluted at 1:1000, BD Bioscience), mouse anti-p53 and rabbit anti-NFκB p65 (diluted at 1:1000, Santa Cruz Biotechnology), mouse anti-HA (diluted at 1:2000, Covance). Anti-mouse and anti-rabbit HRP-conjugated secondary antibodies were from GE Healthcare. Propidium iodide, Alexa-Fluor-594- and Alexa-Fluor-488-conjugated antibodies were from Molecular Probes (Invitrogen).

Lin F., Ghislat G., Luo S., Renna M., Siddiqi F, & Rubinsztein D.C. (2015). XIAP and cIAP1 amplifications induce Beclin 1-dependent autophagy through NFκB activation. Human Molecular Genetics, 24(10), 2899-2913.

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