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Anti vα7.2 3c10

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Anti-Vα7.2 (3C10) is a monoclonal antibody that recognizes the Vα7.2 chain of the T cell receptor (TCR). It can be used for the identification and characterization of Vα7.2+ T cells in flow cytometry applications.

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Lab products found in correlation

Anti cd69 fn50, by Miltenyi Biotec (2 mentions) Anti vα24 c15, by Beckman Coulter (2 mentions) Anti vβ11 c21, by Beckman Coulter (2 mentions) Anti cd16 3g8, by BD (2 mentions) Anti cd161 hp 3g10, by BioLegend (2 mentions) Facscanto 2 cytometer, by BD (2 mentions)

2 protocols using anti vα7.2 3c10

1

Flow Cytometry Immunophenotyping of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell staining and the flow-cytometry-based phenotypic analyses of PBMCs and cells were performed according to standard flow cytometry methods. The following mAbs were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycoerythrin-Cyanine7 (PE/Cy7), phycoerythrin-Cyanine5, phycoerythrin-Cyanine5.5, allophycocyanin (APC), allophycocyanin-Vio7, View blue or View green: anti-CD25 (M-A251), anti-CD27 (M-T271), anti-CD31 (WM59), anti-CD45RA (HI100), anti-CD45RO (UCHL1), anti-CD197/CCR7 (3D12), anti-TCRαβ (T10B9), anti-TCRγδ(B1), anti-CD95 (DX2), anti-CD19 (HIB19), anti-CD21 (B-ly4), anti-IgM (G20-127), anti-IgD (IA6-2), anti-CD56 (B159) and anti-CD16 (3G8), all purchased from BD Biosciences and anti-CD3 (BW264/56), anti-CD4 (VIT4), anti-CD8 (BW135/80) and anti-CD69 (FN50) from Miltenyi Biotec. iNKT cells were detected by staining with anti-Vα24 (C15) and anti-Vβ11 (C21) (Beckman Coulter) and MAIT cells by staining with anti-Vα7.2 (3C10) and anti-CD161 (HP-3G10 (Biolegend). All data were collected on a FACS-Canto II cytometer (BD Biosciences) and analysed using FlowJo Version 9.3.2 software (TreeStar).
Martin E., Palmic N., Sanquer S., Lenoir C., Hauck F., Mongellaz C., Fabrega S., Nitschké P., Esposti M.D., Schwartzentruber J., Taylor N., Majewski J., Jabado N., Wynn R., Picard C., Fischer A., Arkwright P, & Latour S. (2014). CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferation. Nature, 510(7504), 288-292.
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2

Flow Cytometry Immunophenotyping of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell staining and the flow-cytometry-based phenotypic analyses of PBMCs and cells were performed according to standard flow cytometry methods. The following mAbs were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), phycoerythrin-Cyanine7 (PE/Cy7), phycoerythrin-Cyanine5, phycoerythrin-Cyanine5.5, allophycocyanin (APC), allophycocyanin-Vio7, View blue or View green: anti-CD25 (M-A251), anti-CD27 (M-T271), anti-CD31 (WM59), anti-CD45RA (HI100), anti-CD45RO (UCHL1), anti-CD197/CCR7 (3D12), anti-TCRαβ (T10B9), anti-TCRγδ(B1), anti-CD95 (DX2), anti-CD19 (HIB19), anti-CD21 (B-ly4), anti-IgM (G20-127), anti-IgD (IA6-2), anti-CD56 (B159) and anti-CD16 (3G8), all purchased from BD Biosciences and anti-CD3 (BW264/56), anti-CD4 (VIT4), anti-CD8 (BW135/80) and anti-CD69 (FN50) from Miltenyi Biotec. iNKT cells were detected by staining with anti-Vα24 (C15) and anti-Vβ11 (C21) (Beckman Coulter) and MAIT cells by staining with anti-Vα7.2 (3C10) and anti-CD161 (HP-3G10 (Biolegend). All data were collected on a FACS-Canto II cytometer (BD Biosciences) and analysed using FlowJo Version 9.3.2 software (TreeStar).
Martin E., Palmic N., Sanquer S., Lenoir C., Hauck F., Mongellaz C., Fabrega S., Nitschké P., Esposti M.D., Schwartzentruber J., Taylor N., Majewski J., Jabado N., Wynn R., Picard C., Fischer A., Arkwright P, & Latour S. (2014). CTP synthase 1 deficiency in humans reveals its central role in lymphocyte proliferation. Nature, 510(7504), 288-292.
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