CESCs were trypsinized and washed in PBS and stained with the following conjugated antibodies: mouse anti-human CD14-FITC (eBioscience, 11-0149-41), mouse anti-human CD19-FITC (eBioscience, 11-0199-41), mouse anti-human CD34-FITC (eBioscience, 11-0349-41), mouse anti-human CD45-FITC (eBioscience, 11-9459-41), mouse anti-human CD73-FITC (eBioscience, 11-0739-41), mouse anti-human CD90-FITC (eBioscience, 11-0909-41), mouse anti-human CD105-PE (eBioscience, 12-1057-41), mouse anti-human HLA-DR-PerCP-Cyanine 5.5 (eBioscience, 45-9956-41). IgG (mouse IgG1 kappa isotype control-FITC, eBioscience, 11-4714-81; mouse IgG1 kappa isotype control-PE, eBioscience, 12-4714-41; mouse IgG2b kappa isotype control-PerCP-Cyanine 5.5, eBioscience, 45-4732-80) were used as isotype control. The final antibody concentration was at 1 mg/200 mL. After incubating for 30 min at 37°C, the cells were washed three times with PBS. Finally, labeled CESCs were subjected to flow cytometry analysis, and the percentage of positive staining was calculated relative to the isotype control staining.
Mouse igg1 kappa isotype control fitc
Manufactured by Thermo Fisher Scientific
Sourced in United States
The mouse IgG1 kappa isotype control-FITC is a laboratory reagent used to establish background staining levels in flow cytometry experiments. It serves as a negative control to differentiate specific from non-specific antibody binding.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti human cd19 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd34 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd73 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd90 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd105 pe, by Thermo Fisher Scientific (2 mentions)
Mouse anti human hla dr percp cyanine 5, by Thermo Fisher Scientific (2 mentions)
Mouse igg2b kappa isotype control percp cyanine 5, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd14 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse anti human cd45 fitc, by Thermo Fisher Scientific (2 mentions)
Mouse igg1 k isotype control pe, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse igg1 kappa isotype control fitc
1
Immunophenotyping of Cultured Endothelial Stem Cells
2
Immunophenotyping of Cultured Endometrial Stromal Cells
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We trypsinized and washed the the CESCs. Then, the cells were stained with the following antibodies: mouse anti-human CD14-FITC (11-0149-41), mouse anti-human CD19-FITC (11-0199-41), mouse anti-human CD34-FITC (11-0349-41), mouse anti-human CD45-FITC (11-9459-41), mouse anti-human CD73-FITC (11-0739-41), mouse anti-human CD90-FITC (11-0909-41), mouse anti-human CD105-PE (12-1057-41), and mouse anti-human HLA-DR-PerCP-Cyanine 5.5 (45-9956-41), which were purchased from eBioscience (eBioscience, MA, USA). IgG antibodies (mouse IgG1 kappa isotype control-FITC, 11-4714-81; mouse IgG1 kappa isotype control-PE, 12-4714-41; mouse IgG2b kappa isotype control-PerCP-Cyanine 5.5, 45-4732-80; eBioscience, MA, USA) were used as isotype controls. The cells were incubated at 37 °C for 30 min and washed three times with PBS. Finally, CESCs were subjected to flow cytometry analysis, and the percentage of positive staining was calculated.
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