Sodium orthovanadate

Manufactured by MP Biomedicals

Sodium orthovanadate is a chemical compound with the formula Na3VO4. It is a white crystalline solid that is soluble in water. Sodium orthovanadate is commonly used as a protein tyrosine phosphatase inhibitor in biochemical and cell biology research applications.

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Lab products found in correlation

Sodium pyrophosphate decahydrate, by Thermo Fisher Scientific (2 mentions) Sodium fluoride, by Chem-Impex (2 mentions) Bovine serum albumin (bsa), by Avantor (1 mentions) Complete protease inhibitor tablet, by Roche (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Puromycin dihydrochloride, by Merck Group (1 mentions) Securin, by Abcam (1 mentions) Gfp trap magnetic agarose, by Proteintech (1 mentions) Pierce high capacity streptavidin agarose, by Thermo Fisher Scientific (1 mentions) Thymidine, by Chem-Impex (1 mentions) Nocodazole, by Adipogen (1 mentions) Ube2c, by R&D Systems (1 mentions) Hygromycin b, by Thermo Fisher Scientific (1 mentions) S trityl l cysteine, by Thermo Fisher Scientific (1 mentions) Protein g sepharose, by Abcam (1 mentions) Polybrene infection transfection reagent, by Merck Group (1 mentions) Ube2s, by R&D Systems (1 mentions) Streptavidin alexafluor 568, by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)

2 protocols using sodium orthovanadate

Protein Expression and Quantification

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DC Protein Assay kit was purchased from BioRad. SuperSignal West Pico Chemiluminescent Substrate was purchased from Thermo Fisher Scientific. Bovine serum albumin (BSA) was purchased from VWR, and non-fat dry milk was purchased from Laboratory Product Sales. Protease inhibitor (cOmplete) tablets were obtained from Roche. Sodium β-glycerophosphate was obtained from Alfa Aesar, and sodium orthovanadate was purchased from MP Biomedicals. Sodium fluoride was purchased from Chem-Impex International, Inc., and sodium pyrophosphate decahydrate was purchased from Fisher Scientific. Anti-DRD2 (clone B-10, sc-5303), anti-DRD3 (clone 9F4, sc-136170), anti-DRD4 (clone 2B9, sc-136169), anti-PKC-θ (clone E-7, sc-1680), anti-WIP (clone A-7, sc-271113), and anti-N-WASP (clone C-1, sc-271484) were purchased from Santa Cruz Biotechnology. Alexa Fluor 647 mouse anti-WIP (pSer488, clone K32–824, 558674) was purchased from BD Biosciences. Anti-N-WASP (pTyr256, WP2601) was purchased from ECM Biosciences. Anti-mouse horse radish peroxidase-conjugate (170–5947) and anti-GAPDH (clone 6C5, MCA4739) were purchased from BioRad. Anti-β-actin (clone C-4, ICN691002) was purchased from Thermo Fisher Scientific. Anti-α-tubulin (clone B512, T5168) antibody was purchased from EMD Millipore Sigma.

Scott S.A., Fu J, & Chang P.V. (2023). Dopamine receptor D2 confers colonization resistance via gut microbial metabolites. bioRxiv.

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Comprehensive Cell Signaling Assays

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Lipofectamine 2000, Invitrogen (11668019); Lipofectamine RNAiMAX, Invitrogen (13778150); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); Protein G Sepharose, BioVision (6511); Thymidine, Chem-Impex (00306); Propidium iodide, Sigma-Aldrich (P4170); RNase A, Research Products International (R21750); Nocodazole, AdipoGen (AG-CR1-0019); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384); Puromycin dihydrochloride, Sigma-Aldrich (P8833); Blasticidin S hydrochloride, 10 mg/ml in HEPES buffer, Alfa Aesar (J67216); Hygromycin B, 50 mg/mL in PBS, Invitrogen (10687010); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium β-glycerophosphate pentahydrate, Alfa Aesar (L03425); Sodium fluoride, Chem-Impex (01523); Sodium pyrophosphate decahydrate, Fisher (S390); Sodium orthovanadate, MP Bio (0215966410); UBE1, R&D Systems (E-304); UBE2C, R&D Systems (E2-654); UBE2S, R&D Systems (E2-690); securin, Abcam (ab87664); D-biotin, Chem-Impex (00033); Streptavidin Alexa Fluor 568, Invitrogen (S11226); Pierce™ High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); Clarity™ Western ECL Substrate, Bio-Rad (1705061).

Cao X., Shah A.S., Sanford E.J., Smolka M.B., & Baskin J.M. (2022). Proximity labeling reveals spatial regulation of the anaphase-promoting complex/cyclosome by a microtubule adaptor.

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