P27 kip1 d69c12

Dimethyl sulfoxide (DMSO), paraformaldehyde, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), Triton X-100, propidium iodide (PI), DNAse-free RNAse A, perfluorooctanoic acid, cholera toxin (CT), insulin, 3-(4,5-dimethyl-2-yl)2,5-diphenyl-2H-tetrazolium bromide (MTT), epidermal growth factor (EGF), hydrocortisone and sulforaphane were obtained from Sigma-Aldrich (St Louis, MO, USA). Horse serum, penicillin–streptomycin (P/S), Dulbecco’s Phosphate-Buffered Saline (PBS), Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin solution (0.05%) were obtained from Gibco (Invitrogen, Paisley, UK). p53 monoclonal (DO-7), CDK6 monoclonal (75B9), CDK4 monoclonal (DCS-31) and p21 monoclonal (R.229.6) antibodies were obtained from ThermoFisher Scientific (Rockford, IL, USA). P27 Kip1 (D69C12) and cyclin D1 (92G2) antibodies were obtained from Cell Signaling (Danvers, MA, USA). The secondary antibodies Alexa-Fluor 555 goat anti-mouse IgG or 488 goat anti-rabbit IgG, and the blocking agent (normal goat serum) were obtained from Molecular Probes, Invitrogen. Matrigel Basement Membrane Matrix was obtained from Corning (New York, NY, USA). ER α (sc-8002) and ERβ (sc-8974) monoclonal antibodies were obtained from Santa Cruz Biotechnology (Bergheimer, HD, DE). ICI 182,780, GW 6471 was obtained from Tocris Bioscience (Avonmouth, Bristol, UK).

Dimethylsufoxide (DMSO), pharaformaldehyde, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), Triton X-100, Propidium iodide (PI), DNAse-free RNAse A, Perfluorooctanesulfonic acid potassium salt (PFOS), cholera toxin (CT), insulin, epidermal growth factor (EGF), and hydrocortisone were obtained from Sigma-Aldrich (St Louis, MO, USA). Horse serum, penicillin–streptomycin (P/S), Dulbecco’s Phosphate-Buffered Saline (PBS), Dulbecco’s Modified Eagle’s Medium (DMEM), and trypsin solution (0.05%) were obtained from Gibco (Invitrogen, Paisley, UK). p53 monoclonal (DO-7), CDK6 monoclonal (75B9), CDK4 monoclonal (DCS-31), and p21 monoclonal (R.229.6) antibodies were obtained from ThermoFisher Scientific (Rockford, IL, USA). P27 Kip1 (D69C12) and Cyclin D1 (92G2) antibodies were obtained from Cell Signaling (Danvers, MA, USA). The secondary antibodies alexa-fluor 555 goat anti-mouse IgG or 488 goat anti-rabbit IgG, and the blocking agent (normal goat serum) were obtained from Molecular Probes, Invitrogen. Matrigel Basement Membrane Matrix was obtained from Corning (New York, NY, USA). ERα (sc-8002) and ERβ (sc-8974) monoclonal antibodies were obtained from Santa Cruz Biotechnology (Bergheimer, HD, DE). ICI 182,780 was obtained from Tocris Bioscience (Avonmouth, Bristol, UK).