Transsonic 460 h ultrasonic cleaner

The aerial part of F. virosa was gathered from the southern region of Saudi Arabia and identified by field taxonomist “Dr. Mohammed Yusuf of Medicinal Plant Collection and Survey Unit, Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh.” The voucher specimen was deposited in College of Pharmacy, King Saud University. The collected aerial parts were dried, crushed and powdered then sieved through 0.75 mm sieve. Ultrasonication extracted around 50 g of the powdered material in Transsonic-460/H ultrasonic cleaner (ELMA, Germany, having 20 kHz frequency, 100 W power) using methanol as a solvent for 30 min. Since both the markers were found highly soluble in methanol, therefore, methanol has been used as a solvent for extraction. The obtained methanolic extract (ME) was centrifuged for 20 min at 5,000 rpm and filtered through Whatman filter paper. Finally, collected ME of F. virosa (FV) was concentrated through solvent evaporation technique and dried out using rotary evaporator (R-210, BUCHI) at reduced pressure. The calculated yield of FVME was found approximately 9.62%, w/w.

The aerial parts of A. salicina (AS), A. laeta (AL), A. hamulosa (AH), and A. tortilis (AT) were dried in air, powdered and passed through a 0.75 mm sieve. The extraction process took place in a Transsonic-460/H ultrasonic cleaner (ELMA, Germany) according to the Xia et al., 2011 (link). The powdered materials of AS, AL, AH, and AT (50.0 g, each) were extracted by ultrasonication (frequency 20 kHz, power 100 W) using ethanol (95%) for 30 min. The obtained ethanol extracts (ASEE, ALEE, AHEE, and ATEE, respectively) were centrifuged at 5000 rpm for 20 min, filtered through Whatman filter paper No. 1. Therefore, the obtained extracts (ASEE, ALEE, AHEE, and ATEE, respectively) of the four Acacia species (AS, AL, AH, and AT) were concentrated and dried under reduced pressure using rotary evaporator (R-210, BUCHI). The estimated yields (w/w) of ASEE, ALEE, AHEE, and ATEE were 4.81, 5.77, 5.92, and 6.52%, respectively.