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Angiotensin 2

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Angiotensin II is a peptide hormone that plays a crucial role in the regulation of blood pressure and fluid balance. It is a product offered by Bio-Techne, a leading provider of life science research tools and reagents.

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Lab products found in correlation

Unlabeled s1, by AMS Biotechnology (2 mentions) Human ace2, by R&D Systems (2 mentions) Endothelin 1, by Bio-Techne (1 mentions) Phenylephrine, by Merck Group (1 mentions) Sb242084, by Bio-Techne (1 mentions) Exendin 9 39, by Bio-Techne (1 mentions) Para chlorophenylalanine, by Bio-Techne (1 mentions) Exendin 4, by Bio-Techne (1 mentions) R 96544, by Bio-Techne (1 mentions) Liraglutide, by Bachem (1 mentions) Mt 2, by Phoenix Pharmaceuticals (1 mentions) Neutral buffered formalin solution, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Neurobasal a medium, by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Collagenase 2, by Thermo Fisher Scientific (1 mentions) Dispase 2, by Thermo Fisher Scientific (1 mentions) Disbac2 3, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) F12 medium, by Thermo Fisher Scientific (1 mentions)

12 protocols using angiotensin 2

1

Cardiovascular Effects of Angiotensin II Modulators

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A-779 (5-L-isoleucine-7-D-alanine-1-7-angiotensin II, trifluoroacetate salt); AM251 [(N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide)]; angiotensin 1–7; angiotensin II (Tocris Bioscience, Bristol, UK); betadine (Egis Pharmaceuticals PLC, Budapest, Hungary); buprenorphine (Richter Pharma AG, Wels, Austria); CP55940 [(−)-cis-3-[2-hydroxy4-(1,1-dimethylheptyl)phenyl]-trans-4-(3-hydroxypropyl)cyclohexanol] (Sigma Aldrich, St. Louis, MO, USA); losartan potassium (Tocris Bioscience, Bristol, UK); paracetamol (Sequoia, Warsaw, Poland); PD123319 ((6S)-1-[[4-(dimethylamino)-3-methylphenyl]methyl]5-(2,2-diphenylacetyl)-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridine-6-carboxylic acid, di(2,2,2-trifluoroacetate)) (Sigma Aldrich, St. Louis, MO, USA); pentobarbitone sodium (Biowet, Puławy, Poland).
Drugs were dissolved in saline with the following exceptions. AM251 was dissolved in a mixture of ethanol, Cremophor El, DMSO and saline (1:1:1:9.5) for i.v. injections and in DMSO and saline (1:9) for PVN injections. CP55940 was dissolved in a 19% solution of cyclodextrin. None of the used vehicles significantly altered any of the cardiovascular parameters by itself (data not shown).
Mińczuk K., Schlicker E, & Malinowska B. (2022). Cross-Talk between CB1, AT1, AT2 and Mas Receptors Responsible for Blood Pressure Control in the Paraventricular Nucleus of Hypothalamus in Conscious Spontaneously Hypertensive Rats and Their Normotensive Controls. Cells, 11(9), 1542.
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2

Vasoreactive Drug Screening Protocol

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When appropriate, gastrodin (100 μmol/L, dissolved in PBS), RO2959 (Glixx Laboratories, Southborough, MA, USA, GLXC-01511) (5 μmol/L, dissolved in DMSO) were added 12 h before phenylephrine (Sigma-Aldrich, St. Louis, MO, USA, P6126) (50 μmol/L, dissolved in PBS), angiotensin II (Tocris, Bristol, UK, 1158) (10 nmol/L, dissolved in water) and endothelin-1 (Tocris, Bristol, UK,1160) (100 nmol/L, dissolved in water) application. The culture media containing different drugs were renewed every 24 h.
Zheng C., Lo C.Y., Meng Z., Li Z., Zhong M., Zhang P., Lu J., Yang Z., Yan F., Zhang Y., Huang Y, & Yao X. (2017). Gastrodin Inhibits Store-Operated Ca2+ Entry and Alleviates Cardiac Hypertrophy. Frontiers in Pharmacology, 8, 222.
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3

Investigating GLP-1 Receptor Signaling Pathways

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GLP-1 (7-36), Exendin-4 (EX4; GLP-1R agonist), Exendin 9-39 (Ex9-39; GLP-1R antagonist), para-chlorophenylalanine (PCPA), R-96544 (selective 5HT2A antagonist (21 (link))), SB242084 (5HT2C antagonist (22 (link))), and angiotensin II were purchased from Tocris (Bristol, UK). All substances, except for SB242084, Liraglutide, and PCPA, were dissolved in artificial cerebrospinal fluid (aCSF), vehicle for central injections. Liraglutide (Bachem) was dissolved in 0.9% saline. PCPA was dissolved in 0.9% saline by gentle warming and sonication to a concentration of 100 mg/ml (23 (link), 24 (link)). SB242084 was dissolved in 16% DMSO. 5HT2C receptor antagonist SB242084 displays 158- and 100-fold selectivity over 5HT2A and 5HT2B receptors respectively and it also displays selectivity over a range of other 5-HT, dopamine and adrenergic receptors. R-96544 is a potent, selective 5HT2A receptor antagonist; R-96544 shows 100-fold higher affinity for the human 5HT2A receptors than 5HT1A, 5HT1B, 5HT1D, 5HT5A, 5HT6, 5HT7 receptors and 5-HT transporter, although R-96544 has relatively high affinity for 5HT2C receptors (fourfold less compared to 5-HT2A) (21 (link)).
Anderberg R.H., Richard J.E., Eerola K., Lopez-Ferreras L., Banke E., Hansson C., Nissbrandt H., Berqquist F., Gribble F.M., Reimann F., Wernstedt Asterholm I., Lamy C.M, & Skibicka K.P. (2017). Glucagon-like peptide-1 and its analogues act in the dorsal raphe and modulate central serotonin to reduce appetite and body weight. Diabetes, 66(4), 1062-1073.
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4

I-S1 and T-Alb Biodistribution in Mice

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In anesthetized mice, the left jugular vein was exposed for an iv injection of 0.1 mL BSA-LR containing 3×105 cpm of I-S1 (RayBiotech) and 6×105 cpm of T-Alb. For some mice, the injection contained 1 μg/mouse of unlabeled S1 (AMSBIO), mouse acyl ghrelin (CBio, Menlo Park, CA), angiotensin II (Tocris, Bristol, UK), or human ACE2 (R&D, Minneapolis, MN). Ten min after iv injection, whole blood was obtained from the carotid artery and centrifuged after clotting. The whole brain, olfactory bulb, kidney, spleen, and portions of the liver and lung removed. The levels of radioactivity in the arterial serum and the tissues were determined and the results expressed as the percent of the injected I-S1 per mL for serum and delta tissue/serum ratios (μL/g) for the tissues.
Rhea E.M., Logsdon A.F., Hansen K.M., Williams L.M., Reed M.J., Baumann K.K., Holden S.J., Raber J., Banks W.A, & Erickson M.A. (2020). The S1 protein of SARS-CoV-2 crosses the blood–brain barrier in mice. Nature neuroscience, 24(3), 368-378.
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5

Intracerebroventricular Cannulation and MTII Administration in Mice

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Eight-to 10-week-old male mice were stereotaxically implanted with a guide cannula (Plastics1, Roanoake, VA) into the right lateral ventricle (0.4 mm posterior, 1 mm lateral from bregma) under 1–3% (v/v) isoflurane in 1 L/min oxygen. Animals were allowed 6–7 days to recover before the ICV cannula placement was verified through administration of angiotensin II (Tocris, 20 ng in 2 uL) and observation of the dipsogenic response. For all in vivo and gene expression experiments, MTII (Phoenix Pharmaceuticals) was reconstituted in aCSF to a concentration of 500 μM (500 pM/μL), which allowed for delivery of 1 nmol of MTII in 2 μL, aCSF was used as the vehicle control.
Michael N.J., Caron A., Lee C.E., Castorena C.M., Lee S., Zigman J.M., Williams K.W, & Elmquist J.K. (2020). Melanocortin regulation of histaminergic neurons via perifornical lateral hypothalamic melanocortin 4 receptors. Molecular Metabolism, 35, 100956.
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6

Neuronal Cell Culture and Analysis Protocol

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DMEM medium, Neurobasal A medium, F-12 medium, Fetal Bovine Serum (FBS), HBSS, D-PBS, Penicillin/Streptomycin, B-27, Collagenase II, Dispase II, Trypsin, Alexa Fluor® 647 conjugated anti-rabbit antibody, and DiSBAC2(3) were purchased from Life Technologies (Carlsbad, CA, USA). DNAse 1, 30% BSA, Cytosine β-d-arabinofuranoside hydrochloride (ARA-c), 5-Fluoro-2′-deoxyuridine 5′-monophosphate sodium salt (FdU) solution (FdU), poly-d-Lysine, DAPI, and 10% neutral buffered formalin solution were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Angiotensin II was purchased from Tocris (Bristol, UK). C21 (MW = 475.2) and EMA401 (MW = 507.2) were synthesized by AGV discovery (Clapiers, France), purified by HPLC (>95%), and characterized by 1H NMR (Figures S3 and S4) and mass spectrometry. Anti-rabbit β3-Tubulin (D71G9) XP® (anti-Tuj1) was purchased from Cell Signaling Technologies® (Danvers, MA, USA). Micro-titer plate (384-well), µClear®, was purchased from Greiner Bio-One SAS (Les Ulis, France). Purified mycolactone was obtained and quantified as described in a previous study [9 (link)], and re-suspended in DMSO at 5 mg/mL and stored at −20 °C as aliquots in amber glass tubes.
Song O.R., Kim H.B., Jouny S., Ricard I., Vandeputte A., Deboosere N., Marion E., Queval C.J., Lesport P., Bourinet E., Henrion D., Oh S.B., Lebon G., Sandoz G., Yeramian E., Marsollier L, & Brodin P. (2017). A Bacterial Toxin with Analgesic Properties: Hyperpolarization of DRG Neurons by Mycolactone. Toxins, 9(7), 227.
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7

Angiotensin-II and Forskolin Response

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H295R cells were transferred to 12 wells dishes in groups of 600,000 cells per well, and maintained at the conditions described. After 24h passage, DMEM/Eagle’s F12 medium supplemented with 0.1% CCS and, after 48h, DMEM/Eagle’s F12 media containing angiotensin-II (Tocris, Bristol, United Kingdom) (10nM), and forskolin (Tocris) (10 μM) were added to different groups of cells, each group comprising 3 wells. A basal group, to which no drug was added, was used a control. RNA was extracted at 3, 6, 12, and 24h time points (RNeasy Mini Kit, QIAGEN, Hilden, Germany). All the cell experiments were independently conducted in triplicate, with cells raised at different times.
Felizola S.J., Nakamura Y., Ono Y., Kitamura K., Kikuchi K., Onodera Y., Ise K., Takase K., Sugawara A., Hattangady N., Rainey W.E., Satoh F, & Sasano H. (2014). PCP4: a regulator of aldosterone synthesis in human adrenocortical tissues. Journal of molecular endocrinology, 52(2), 159-167.
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8

Olanzapine and Angiotensin II Protocol

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Olanzapine (PubChem CID: 4585) was a generous gift of Neuland Laboratories Ltd (Hyderabad, India). Angiotensin II (PubChem CID: 172198) and rimonabant (RIM; PubChem CID: 104850) were purchased from Tocris Bioscience (Minneapolis, Minnesota). Angiotensin II was used at the end of the experiment to verify that the intracerebroventricular (ICV) cannulas were placed correctly by performing a water drinking test.
Klingerman C.M., Stipanovic M.E., Hajnal A, & Lynch C.J. (2015). Acute Metabolic Effects of Olanzapine Depend on Dose and Injection Site. Dose-Response, 13(4), 1559325815618915.
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9

I-S1 and T-Alb Biodistribution in Mice

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In anesthetized mice, the left jugular vein was exposed for an iv injection of 0.1 mL BSA-LR containing 3×105 cpm of I-S1 (RayBiotech) and 6×105 cpm of T-Alb. For some mice, the injection contained 1 μg/mouse of unlabeled S1 (AMSBIO), mouse acyl ghrelin (CBio, Menlo Park, CA), angiotensin II (Tocris, Bristol, UK), or human ACE2 (R&D, Minneapolis, MN). Ten min after iv injection, whole blood was obtained from the carotid artery and centrifuged after clotting. The whole brain, olfactory bulb, kidney, spleen, and portions of the liver and lung removed. The levels of radioactivity in the arterial serum and the tissues were determined and the results expressed as the percent of the injected I-S1 per mL for serum and delta tissue/serum ratios (μL/g) for the tissues.
Rhea E.M., Logsdon A.F., Hansen K.M., Williams L.M., Reed M.J., Baumann K.K., Holden S.J., Raber J., Banks W.A, & Erickson M.A. (2020). The S1 protein of SARS-CoV-2 crosses the blood–brain barrier in mice. Nature neuroscience, 24(3), 368-378.
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10

Exendin-4, GLP-1, and Angiotensin II Protocol

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Exendin-4 (Ex4), GLP-1 (7–36) and angiotensin II, were purchased from Tocris (Bristol, UK) dissolved in aCSF (vehicle for all central injections) and stored as aliquots in -20°C.
Richard J.E., Anderberg R.H., Göteson A., Gribble F.M., Reimann F, & Skibicka K.P. (2015). Activation of the GLP-1 Receptors in the Nucleus of the Solitary Tract Reduces Food Reward Behavior and Targets the Mesolimbic System. PLoS ONE, 10(3), e0119034.
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