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Phycoerythrin pe labeled streptavidin

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Phycoerythrin (PE)–labeled streptavidin is a fluorescent protein complex used in various biotechnological applications. Streptavidin is a tetrameric protein that binds strongly to the vitamin biotin, while PE is a brightly fluorescent protein derived from red algae. The combination of these two components creates a versatile tool for labeling and detection in techniques such as flow cytometry, immunoassays, and microscopy.

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Close spelling variants of this product

Streptavidin-PE (163 citations) Phycoerythrin (PE) (16 citations) Streptavidin-phycoerythrin (11 citations) Streptavidin-phycoerythrin (PE) (10 citations) PE)-labeled streptavidin (10 citations)

2 protocols using phycoerythrin pe labeled streptavidin

1

Biotinylated ASKP1240 Antibody Evaluation

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A biotinylated ASKP1240 antibody and anti-ASKP1240 serum were kindly supplied by Kyowa Hakko Kirin Co., Ltd. The pooled normal Cynomolgus monkey sera were kindly supplied by Shin Nippon Biomedical Laboratories, Ltd. Allophycocyanin (APC)–labeled antihuman CD20 mAb (2H7) and phycoerythrin (PE)–labeled streptavidin were purchased from BD Biosciences-Pharmingen, Canada.
Ma A., Dun H., Song L., Hu Y., Zeng L., Bai J., Zhang G., Kinugasa F., Miyao Y., Sakuma S., Okimura K., Kasai N., Daloze P, & Chen H. (2014). Pharmacokinetics and Pharmacodynamics of ASKP1240, a Fully Human Anti-CD40 Antibody, in Normal and Renal Transplanted Cynomolgus Monkeys. Transplantation, 97(4), 397-404.
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2

PD-L1 and PD-L2 Expression Analysis

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Cells were stained with either biotinylated mouse monoclonal antibodies to human PD-L1 (eBioscience, San Diego, CA) or an immunoglobulin G1 k isotype control (eBioscience), followed by phycoerythrin (PE)labeled streptavidin (BD Biosciences, San Jose, CA), or with PE-labeled mouse monoclonal antibodies to human PD-L2 (BioLegend, San Diego, CA) or a PE-labeled immunoglobulin G2a k isotype control (BioLegend), for flow cytometric analysis with a fluorescence-activated cell sorting Verse instrument (BD Biosciences). The relative median fluorescence intensity (MFI) ratio was calculated as PD-L1 or PD-L2 MFI divided by isotype control MFI.
Shibahara D., Tanaka K., Iwama E., Kubo N., Ota K., Azuma K., Harada T., Fujita J., Nakanishi Y, & Okamoto I. (2018). Intrinsic and Extrinsic Regulation of PD-L2 Expression in Oncogene-Driven Non-Small Cell Lung Cancer. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 13(7).
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