Fluorescent probe 2,7-dichlorofluorescein diace-tate (DCF-DA), 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT), Triton X-100, FBS and Rhodamine-123 were purchased from Sigma(St Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM-F12) was purchased from Gibco (Gibco, Grand Island, NY, USA). Caspase-3 Detection Kit was provided from Sigma. Super Oxide Dismutase Assay Kits were purchased from Cayman (Ann Arbor, MI, USA). LiChroprep® RP-18 (15-25 μm) were purchased from Merck (Darmstadt, Germany) and all the solvents used for extraction from Scharlau and Caledon (Sentmenate, Spain).
Super oxide dismutase assay kits
Manufactured by Cayman Chemical
Sourced in United States
The Super Oxide Dismutase Assay Kits are laboratory equipment designed to measure the activity of the enzyme superoxide dismutase (SOD) in biological samples. The kits provide a quantitative colorimetric method for determining SOD levels, which is useful for assessing oxidative stress and antioxidant capacity in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Glutathione peroxidase assay kit, by Cayman Chemical (2 mentions)
Triton x 100, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Caspase 3 detection kit, by Merck Group (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
Malondialdehyde, by Cayman Chemical (1 mentions)
Kc4 version 3, by Agilent Technologies (1 mentions)
Glutathione assay kit, by Cayman Chemical (1 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Superoxide dismutase assay kit, by Cayman Chemical (1 mentions)
4 protocols using super oxide dismutase assay kits
1
Oxidative Stress and Apoptosis Assay
2
Cellular Oxidative Stress Assays
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After treatment, cells were collected, and cellular malondialdehyde and superoxide dismutase contents were determined using malondialdehyde as well as superoxide dismutase assay kits (Cayman Chemical Co), respectively, according to the manufacturer’s instruction. Loss of mitochondrial membrane potential was assessed by 5,5’,6,6’‐tetrachloro‐1,1’,3,3’‐tetraethylbenzimidazolylcarbocyanine iodide (JC‐1) (Beyotime) fluorescence probe.14 In brief, after treatment, cells were washed with PBS and stained with JC‐1 for 20 minutes at 37 ℃. Cells were then rinsed twice with warm DMEM, incubated in 1 ml of culture medium, and visualized under a fluorescence microscope (Olympus). Fluorescence intensity was quantified using ImageJ software (National Institutes of Health).
3
Antioxidant Enzyme Activity Assays
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Antioxidant activities of Superoxide dismutase (SOD ), Glutathione peroxidase (GPx ), and Glutathione reductase (GR ) were assessed using the Superoxide dismutase Assay Kits (Item No. 706002), Glutathione Peroxidase Assay Kit (Item No. 703102), Glutathione Reductase Assay Kits (Item No. 703202), respectively, following the manufacturers’ instructions (Cayman Chemical Company, Ann Arbor, MI). Absorbance for serum SOD was measured at 450 nm while absorbance for plasma GPx and GR were read once every minute at 340 nm using a plate reader (Bio-Tek Instrument Inc., Winooski, VT) and a software program (KC4, version #3.3, Bio Tek Instruments) to obtain at least 5-time points. The concentration of GPx and GR were expressed as nmol/min/mL while SOD was expressed as U/mL equivalent.
4
Antioxidant Enzyme Assays in Avian Liver
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On d 20 and 26 (6 and 12 dpi, respectively), liver samples were collected from the selected bird and kept at −80°C, and the activities of glutathione peroxidase (GPx ) and superoxide dismutase (SOD ) and concentration of reduced and oxidized glutathione (GSH and GSSG , respectively) were measured. The Glutathione Peroxidase Assay Kits (Cayman Chemical, Glutathione Peroxidase Assay Kits, item No. 703102, Ann Arbor, MI), Superoxide Dismutase Assay Kits (Cayman Chemical, Superoxide Dismutase Assay Kit, item No. 706002, Ann Arbor, MI), and Glutathione Assay Kits (Cayman Chemical, Glutathione Assay Kit, item No. 703002, Ann Arbor, MI) were used. Sample preparation and assay were performed according to the manufacturer's instructions. Additionally, a protein quantification assay kit (Pierce BCA Protein Assay Kit, Ref. 23227, Thermo Scientific, Rockford, IL) was used to standardize the samples. The procedure followed the manufacturer's instructions, and bovine serum albumin (2 mg/mL) was used as the protein standard.
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