Wild type

All procedures using animal subjects were conducted in accordance with the standards of the Institute of Animal Care and Use Committee (IACUC) at Western Michigan University. Wild-type (Jackson Laboratories, Bar Harbor, ME) and transgenic 129/SvJ mice (3–6 months old, both sexes) weighing between 22 and 25 g were used for these studies. Animals were housed in a normal experimental room and exposed to 12-hour light/dark cycle with free access to food and water. 129/SvJ mice carrying the Rlbp^Cre-ER (Cre⁺) and Rosa-tdTomato^floxstopflox (tdTomato⁺) transgenes (generated and used with permission by E. Levine, Vanderbilt Univ.) were used to label Müller glia (MG) (Vazquez-Chona et al., 2009 (link); Webster et al., 2019 (link)). Cre^+/WT;tdTomato^+/− mice were injected with tamoxifen, leading to tdTomato reporter expression in MG cells of the retina, as previously reported (Webster et al., 2019 (link)). Specifically, 6-week old male and female mice were injected intraperitoneally with 10 mg/mL tamoxifen in corn oil for three subsequent days for a total injection volume of 300μl tamoxifen. To generate experimental mice, Cre^+/WT;tdTomato^+/− males were crossed with Cre^WT/WT;tdTomato^+/+ females. Pups were tagged, tail-clipped, and genotyped by PCR.

Wild-type and Nur77^−/− mice (male, 4–6 weeks old, C57BL/6 background) were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA). The protocols for animal studies were approved by the Animal Care and Use Committee of Xiamen University, and all mice were handled in accordance with the “Guide for the Care and Use of Laboratory Animals” and the “Principles for the Utilization and Care of Vertebrate Animals.” Animals were used for isolating cultures of primary cells from mice. All cell lines are described in the key Resources Table.