Anti tβri

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TβRI is a primary antibody that recognizes the TGF-beta receptor type I (TβRI) protein. TβRI is a transmembrane serine/threonine kinase receptor that is a key component of the TGF-beta signaling pathway. This antibody can be used to detect and study the expression and distribution of TβRI in various biological samples.

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Lab products found in correlation

2 protocols using anti tβri

Immunoblot Analysis of EMT Markers

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The antibodies used in the present study included anti-human E-cadherin (1:500, Santa Cruz Biotechnology Inc.), anti-human N-cadherin (1:1,000, BD Bioscience), anti-α-SMA (1:300, Abcam, Cambridge, UK), anti–β-actin (1:5,000, Sigma-Aldrich), anti-Smad3, anti-pSmad3 (1:1,000, Cell Signaling Technology, Danvers, MA, USA), anti-ERK1/2, anti-pERK1/2 (1:1,000, Cell Signaling Technology), anti-p38 mitogen-activated protein kinase (MAPK), anti-pp38 MAPK (1:1,000, Cell Signaling Technology), anti-TβRI (1:1,000, Santa Cruz Biotechnology Inc.), and anti-TβRII (1:500, Abcam).

Baek A.R., Lee J.M., Seo H.J., Park J.S., Lee J.H., Park S.W., Jang A.S., Kim D.J., Koh E.S., Uh S.T., Kim Y.H, & Park C.S. (2016). Apolipoprotein A1 Inhibits TGF-β1–Induced Epithelial-to-Mesenchymal Transition of Alveolar Epithelial Cells. Tuberculosis and Respiratory Diseases, 79(3), 143-152.

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Antibody Characterization for Cellular Pathways

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The following antibodies were used in this study: anti-SARA [mouse, sc-133071; 1:200 for immunoblotting and 1:100 for immunofluorescence (IF)], anti-PP1c (mouse, sc-7482; 1:50 for IF), anti-GADD34 (mouse, sc-373815; 1:50 for IF), anti-TβRI (mouse, sc-101574; 1:100 for IF) and anti-Smad2/3 (mouse, sc-398844; 1:100 for IF); all these antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, United States). The antibody anti-MAP2 (rabbit, 1:500) and anti-Tau-1 (mouse, 1:500) were from Merck Millipore (Darmstadt, Germany). Antibody anti-pSamd2/3 (rabbit, s465/s467, E8F3R, 1:50 for IF) was from Cell Signaling (Danvers, MA, United States) and anti-phospho TβRI (rabbit, ser165, Lot: DY1241; 1:50 for IF) was acquired from Elabscience (Houston, Texas, United Sates).

Rozés-Salvador V., Wilson C., Olmos C., Gonzalez-Billault C, & Conde C. (2020). Fine-Tuning the TGFβ Signaling Pathway by SARA During Neuronal Development. Frontiers in Cell and Developmental Biology, 8, 550267.

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