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2 protocols using hyd hcl

1

Topoisomerase Inhibitors and Oxidative Stress

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B16-F1 and B16-F10 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Topoisomerase inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA; camptothecin, merbarone, teniposide, etoposide, XK469, ICRF-193) and Cayman Chemical (Ann Arbor, MI, USA; doxorubicin, moxifloxacin). NAC, LPA and HYD HCl were from Sigma-Aldrich. Fluorescent probes were from Life Technologies (Waltham, MA, USA; DDAOG, C11-BODIPY, CellROX Green, CellTrace Violet), eBioscience (San Diego, CA, USA; CV450) and Sigma-Aldrich (DAPI). Bafilomycin A1 was from Research Products International (Mount Prospect, IL, USA). Primary antibodies were from Millipore (Billerica, MA, USA; anti-γH2AX, clone JBW301) or Abcam (Cambridge, UK; anti-4-HNE, anti-OHdG). Fluorescent secondary antibodies were obtained from Thermo Pierce (Waltham, MA, USA). Cell culture reagents were from Life Technologies (DMEM, pen-strep solution, trypsin-EDTA) and Gemini Biosciences (West Sacramento, CA, USA; fetal bovine serum, stabilized l-glutamine).
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2

Topoisomerase Inhibitors and Oxidative Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols
B16-F1 and B16-F10 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). Topoisomerase inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA; camptothecin, merbarone, teniposide, etoposide, XK469, ICRF-193) and Cayman Chemical (Ann Arbor, MI, USA; doxorubicin, moxifloxacin). NAC, LPA and HYD HCl were from Sigma-Aldrich. Fluorescent probes were from Life Technologies (Waltham, MA, USA; DDAOG, C11-BODIPY, CellROX Green, CellTrace Violet), eBioscience (San Diego, CA, USA; CV450) and Sigma-Aldrich (DAPI). Bafilomycin A1 was from Research Products International (Mount Prospect, IL, USA). Primary antibodies were from Millipore (Billerica, MA, USA; anti-γH2AX, clone JBW301) or Abcam (Cambridge, UK; anti-4-HNE, anti-OHdG). Fluorescent secondary antibodies were obtained from Thermo Pierce (Waltham, MA, USA). Cell culture reagents were from Life Technologies (DMEM, pen-strep solution, trypsin-EDTA) and Gemini Biosciences (West Sacramento, CA, USA; fetal bovine serum, stabilized l-glutamine).
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