Tdt mediated dutp nick end labeling tunel apoptosis assay kit

ConA and Fucoidan from Fucus vesiculosus were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies were provided by Cell Signaling Technology (Danvers, MA, USA) including the antibodies against iNOS, Bid, Bax, Bcl-2, Bcl-xL, TRADD, TRAF2, FADD, p-FADD, JAK2, p-JAK2, STAT1, and p-STAT1. The TNF-α and IFN-γ antibodies were purchased from Abcam (Cambridge, MA, USA) and Caspase 3, 8 and 9 were purchased from Proteintech (Chicago, IL, USA). The RNA polymerase chain reaction (PCR) kit was purchased from Takara Biotechnology (Dalian, China). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) microplate test kits were produced by Nanjing Jiancheng Bioengineering Institute (Jiancheng Biotech, China). The enzyme-linked immunosorbent assay (ELISA) kits were acquired from eBioscience (San Diego, CA, USA). The TdT-mediated dUTP nick end labeling (TUNEL) apoptosis assay kit was purchased from Roche (Roche Ltd, Basel, Switzerland).

ConA and OA were obtained from Sigma-Aldrich (St. Louis, MO, USA). The alanine aminotransferase (ALT) and the aspartate aminotransferase (AST) microplate test kits were obtained from the Nanjing Jiancheng Bioengineering Institute (Jiancheng Biotech, China). The enzyme-linked immunosorbent assay (ELISA) kits were acquired from eBioscience (San Diego, CA, USA). The RNA polymerase chain reaction (PCR) kit was purchased from Takara Biotechnology (Dalian, China). The antibodies for PPARα, JNK, p-JNK, TRAF2, Bax, Bcl-2, LC3, Beclin 1, and caspase-3 were provided by Proteintech (Chicago, IL, USA). The IL-1β, IL-6, and TNF-α antibodies were from Abcam (Cambridge, MA, USA). The TdT-mediated dUTP nick end labeling (TUNEL) apoptosis assay kit was from Roche (Roche Ltd, Basel, Switzerland).

Fenofibrate was purchased from Kingmorn Life Science (Shanghai, China) and suspended with 10% DMSO. ALT and AST regent kits were obtained from Jiancheng Bioengineering Institute (Nanjing, China). The enzyme-linked immunosorbent assay (ELISA) kits were acquired from eBioscience (San Diego, CA, USA). The RNA polymerase chain reaction (PCR) kit was from Takara Biotechnology (Dalian, China). The antibodies for PPAR-α, IL-6, Bax, Bcl-2, Beclin-1, P62, LC3, Casepase-9, Caspase-3, and Nrf-2 were provided by Proteintech (Chicago, IL, USA). The TNF-α and IL-1β antibodies were from Abcam (Cambridge, MA, USA). The AMPK and p-AMPK antibodies were from CST (Danvers, MA, USA). The TdT-mediated dUTP nick end labeling (TUNEL) apoptosis assay kit was from Roche (Roche Ltd., Basel, Switzerland).